The USC Norris Comprehensive Cancer Center (NCCC) will continue in its mission to support basic research on the molecular genetic mechanisms underlying cancer, and translational research aimed at using discoveries in basic science to improve patient care. Now in its 17'*^ year of operation, the Transgenic/Knockout Mouse Core continues to offer a full range of mouse genome manipulation services. These include the targeting of genes in ES cells, the generation of gene targeted mice by injection of ES cells into blastocysts, and the production of transgenic mice by pronuclear injection. In addition to these major services, the Core also offers a number of ancillary services, including cryopreservation of embryos, rederivation of mouse strains, and in vitro fertilization. Finally, in the long term, the Core proposes to offer ES cell injection and gene knockouts in rats. In addition to funding from the Cancer Center Core Grant, the Core receives a large contribution from the Keck School of Medicine ($150,000 in FY 09-10, projected to continue yearly).The Core also receives support from the Zilkha Neurogenetics Institute ($25,000) and the College of Letters, Arts, and Sciences ($10,000). This broad support from several USC schools/Institutes leverages the CCCG contribution, enabling the Core to continue to offer a wide range of services at competitive prices to Cancer Center investigators. The Core is directed by Robert Maxson, Ph.D. Professor of Biochemistry and Molecular Biology (5% FTE). It is staffed by Dr. Nancy Wu (90%), an expert mouse embryologist and injectionist with 17 years of experience;and Dr. Mandy Ting (50%), an expert in ES cell manipulation. Assisting with all of Core functions is Youzhen Yan (90%) and John Johnson (10%). Core policies, including pricing, are set by a User's Committee in consultation with the Cancer Center Executive Committee. Outside prices are established by cost analysis. Prices for in house investigators are reduced from outside prices, depending on the academic affiliation of the investigator and the extent to which the investigator's unit supports the Core. Prices are listed online on the Core's website in the NCCC Shared Resources webpage. Also on the Core's website are order forms for Core services. The Core at its current staffing of 2.45 FTEs has the ability to perform four to six pronuclear injections, one gene targeting in ES cells, and one to two injections of ES cells into blastocysts to produce targeted mice.

Public Health Relevance

Access for NCCC investigators to state-of-the-art transgenic and knockout mouse technology is fundamental to the dual goals of supporting basic research on the molecular genetic mechanisms underlying cancer and translational research aimed at using discoveries in basic science to improve patient care. This technology is a virtual requirement for understanding the roles of specific genes in oncogenic processes, and is also increasingly important in translational studies on potential new therapies.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA014089-39
Application #
8589366
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2013-12-01
Budget End
2014-11-30
Support Year
39
Fiscal Year
2014
Total Cost
$117,937
Indirect Cost
$42,915
Name
University of Southern California
Department
Type
DUNS #
072933393
City
Los Angeles
State
CA
Country
United States
Zip Code
90089
Battaglin, Francesca; Naseem, Madiha; Puccini, Alberto et al. (2018) Molecular biomarkers in gastro-esophageal cancer: recent developments, current trends and future directions. Cancer Cell Int 18:99
Zhang, Junjie; Zhao, Jun; Xu, Simin et al. (2018) Species-Specific Deamidation of cGAS by Herpes Simplex Virus UL37 Protein Facilitates Viral Replication. Cell Host Microbe 24:234-248.e5
Eriguchi, Yoshihiro; Nakamura, Kiminori; Yokoi, Yuki et al. (2018) Essential role of IFN-? in T cell-associated intestinal inflammation. JCI Insight 3:
Cobo, Eduardo R; Holani, Ravi; Moreau, France et al. (2018) Entamoeba histolytica Alters ileal Paneth Cell Functions in Intact and Muc2 Mucin Deficiency. Infect Immun :
Suenaga, Mitsukuni; Schirripa, Marta; Cao, Shu et al. (2018) Gene Polymorphisms in the CCL5/CCR5 Pathway as a Genetic Biomarker for Outcome and Hand-Foot Skin Reaction in Metastatic Colorectal Cancer Patients Treated With Regorafenib. Clin Colorectal Cancer 17:e395-e414
Thomas, Nancy E; Edmiston, Sharon N; Orlow, Irene et al. (2018) Inherited Genetic Variants Associated with Melanoma BRAF/NRAS Subtypes. J Invest Dermatol 138:2398-2404
Rhie, Suhn Kyong; Yao, Lijun; Luo, Zhifei et al. (2018) ZFX acts as a transcriptional activator in multiple types of human tumors by binding downstream of transcription start sites at the majority of CpG island promoters. Genome Res :
Pinto, Navin; DuBois, Steven G; Marachelian, Araz et al. (2018) Phase I study of vorinostat in combination with isotretinoin in patients with refractory/recurrent neuroblastoma: A new approaches to Neuroblastoma Therapy (NANT) trial. Pediatr Blood Cancer 65:e27023
Hanna, Diana L; Loupakis, Fotios; Yang, Dongyun et al. (2018) Prognostic Value of ACVRL1 Expression in Metastatic Colorectal Cancer Patients Receiving First-line Chemotherapy With Bevacizumab: Results From the Triplet Plus Bevacizumab (TRIBE) Study. Clin Colorectal Cancer 17:e471-e488
Müller, Fabian; Cunningham, Tyler; Stookey, Stephanie et al. (2018) 5-Azacytidine prevents relapse and produces long-term complete remissions in leukemia xenografts treated with Moxetumomab pasudotox. Proc Natl Acad Sci U S A 115:E1867-E1875

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