Mass Spectrometry Core: Proteomics and Metabolomics Shared Resource - Project Summary/Abstract Since the last renewal, the Mass Spectrometry Core: Proteomics and Metabolomics has been significantly upgraded, including a new Director and staff, the purchase of state-of-the art instrumentation, expansion of Core proteomics services, and the introduction of metabolomics services. By improving every aspect of service, the Mass Spectrometry Core provides Cancer Center members with the highest level of support for their proteomic, metabolomic, and custom peptide synthesis needs. The Director and Associate Director provide expert consultation concerning the design, implementation, and analysis of a wide range of experiments including: protein identification, protein quantification, identification of post-translational modifications, secreted protein/peptide analysis, untargeted lipidomics profiling, targeted lipidomics and metabolomics, bile acids analysis, amino acid quantitation, fatty acid analysis, and custom peptide synthesis. In recent years, five new mass spectrometers have been acquired and a highly experienced Core staff, with strong working knowledge of a wide range of proteomics and metabolomics applications, has been recruited to oversee this facility. The staff provides the knowledge and technical skills to get the most out of this sophisticated new equipment. The Core staff are constantly working to implement new analytical techniques in these rapidly evolving fields while supporting researchers with data analysis, as well as manuscript and grant preparation. Peptide synthesis services have recently been consolidated into this Core and offer custom services for synthesis, HPLC purification, and mass spectrometry-based analysis of peptides, including long peptide syntheses and custom modifications. This Core is broadly used by the vast majority of Cancer Center members, and the staff remain dedicated to meeting and advancing their research. The Core strives to facilitate cancer research by: 1) providing sophisticated instrumentation for discovery: the Core has five high- resolution mass spectrometers (three for proteomics and two for metabolomics) and one peptide synthesizer, 2) providing expertise: decades of combined experience from the Core staff are made readily accessible to Salk investigators for consultations concerning experimental design, data acquisition and analysis, and manuscript and grant preparation, 3) providing critical services: The Core staff provides reproducible, high- quality sample preparation services prior to metabolic and proteomic analysis, as well as providing custom peptide synthesis and purification, 4) providing education, training, and support: Salk researchers at all levels of expertise can receive training in the successful applications of mass spectrometry for proteomic and metabolomics analysis, particularly with respect to experimental design and data analysis, and 5) identifying and evaluating new technologies: the Core seeks to ensure that it continuously offers state-of-the-art instrumentation and validated technological advances to enhance discovery as a result of the Core users' experiments.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA014195-48
Application #
10114242
Study Section
Subcommittee I - Transistion to Independence (NCI)
Project Start
1996-12-31
Project End
2024-01-31
Budget Start
2021-02-01
Budget End
2022-01-31
Support Year
48
Fiscal Year
2021
Total Cost
Indirect Cost
Name
Salk Institute for Biological Studies
Department
Type
DUNS #
078731668
City
La Jolla
State
CA
Country
United States
Zip Code
92037
Zarrinpar, Amir; Chaix, Amandine; Xu, Zhenjiang Z et al. (2018) Antibiotic-induced microbiome depletion alters metabolic homeostasis by affecting gut signaling and colonic metabolism. Nat Commun 9:2872
Ramaswamy, Suvasini; Tonnu, Nina; Menon, Tushar et al. (2018) Autologous and Heterologous Cell Therapy for Hemophilia B toward Functional Restoration of Factor IX. Cell Rep 23:1565-1580
Hsu, Cynthia L; Lee, Elian X; Gordon, Kara L et al. (2018) MAP4K3 mediates amino acid-dependent regulation of autophagy via phosphorylation of TFEB. Nat Commun 9:942
Sonntag, Tim; Vaughan, Joan M; Montminy, Marc (2018) 14-3-3 proteins mediate inhibitory effects of cAMP on salt-inducible kinases (SIKs). FEBS J 285:467-480
Herzig, Sébastien; Shaw, Reuben J (2018) AMPK: guardian of metabolism and mitochondrial homeostasis. Nat Rev Mol Cell Biol 19:121-135
Sweeney, Lora B; Bikoff, Jay B; Gabitto, Mariano I et al. (2018) Origin and Segmental Diversity of Spinal Inhibitory Interneurons. Neuron 97:341-355.e3
Hartmann, Phillipp; Hochrath, Katrin; Horvath, Angela et al. (2018) Modulation of the intestinal bile acid/farnesoid X receptor/fibroblast growth factor 15 axis improves alcoholic liver disease in mice. Hepatology 67:2150-2166
Glustrom, Leslie W; Lyon, Kenneth R; Paschini, Margherita et al. (2018) Single-stranded telomere-binding protein employs a dual rheostat for binding affinity and specificity that drives function. Proc Natl Acad Sci U S A 115:10315-10320
Giraddi, Rajshekhar R; Chung, Chi-Yeh; Heinz, Richard E et al. (2018) Single-Cell Transcriptomes Distinguish Stem Cell State Changes and Lineage Specification Programs in Early Mammary Gland Development. Cell Rep 24:1653-1666.e7
Ma, Jiao; Saghatelian, Alan; Shokhirev, Maxim Nikolaievich (2018) The influence of transcript assembly on the proteogenomics discovery of microproteins. PLoS One 13:e0194518

Showing the most recent 10 out of 457 publications