LUCIFERASE MOLECULAR IN VIVO IMAGING FACILITY Mark W. Dewhirst, DVM, PhD, Director Molecular imaging of gene expression in vivo in real time at the cellular level is a rapidly growing area of cancer research that will provide insight into the origin and progression of cancer at the genetic level. The ability to obtain spatial and functional information on gene expression at the cellular level, and to follow gene expression in real time in the same group of animals, will provide insights not previously possible with classical histopathology techniques. The Xenogen Corporation MS in vivo bioluminescence imaging system is recognized as being at the forefront of this emerging field. The long-term goal of this plan is to establish a comprehensive shared imaging facility at Duke University Medical Center to provide cancer researchers access to this cutting-edge technology. In the past year, cancer researchers at Duke have begun to explore the potential of bioluminescence imaging in projects such as non-invasive monitoring of metastatic tumor growth, expression patterns of adenoviral gene therapy vectors following intratumoral administration, long term expression patterns of genes following AAV administration, and sm'vival of tumor cells after irradiation. In this grant, we seek the resources to enable the expansion of the current facility to include imaging of immunodeficient animals, and to upgrade the technical capabilities of the existing system in order to provide Duke researchers with the best available tools for their studies.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA014236-33
Application #
7726626
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2007-01-01
Budget End
2007-12-31
Support Year
33
Fiscal Year
2007
Total Cost
$24,516
Indirect Cost
Name
Duke University
Department
Type
DUNS #
044387793
City
Durham
State
NC
Country
United States
Zip Code
27705
Duan, Bensong; Hu, Jiangfeng; Liu, Hongliang et al. (2018) Genetic variants in the platelet-derived growth factor subunit B gene associated with pancreatic cancer risk. Int J Cancer 142:1322-1331
Wu, Mengxi; Huang, Po-Hsun; Zhang, Rui et al. (2018) Circulating Tumor Cell Phenotyping via High-Throughput Acoustic Separation. Small 14:e1801131
Vlahovic, Gordana; Meadows, Kellen L; Hatch, Ace J et al. (2018) A Phase I Trial of the IGF-1R Antibody Ganitumab (AMG 479) in Combination with Everolimus (RAD001) and Panitumumab in Patients with Advanced Cancer. Oncologist 23:782-790
Xu, Yinghui; Liu, Hongliang; Liu, Shun et al. (2018) Genetic variant of IRAK2 in the toll-like receptor signaling pathway and survival of non-small cell lung cancer. Int J Cancer 143:2400-2408
Feng, Yun; Wang, Yanru; Liu, Hongliang et al. (2018) Novel genetic variants in the P38MAPK pathway gene ZAK and susceptibility to lung cancer. Mol Carcinog 57:216-224
Naqvi, Ibtehaj; Gunaratne, Ruwan; McDade, Jessica E et al. (2018) Polymer-Mediated Inhibition of Pro-invasive Nucleic Acid DAMPs and Microvesicles Limits Pancreatic Cancer Metastasis. Mol Ther 26:1020-1031
Wen, Juyi; Liu, Hongliang; Wang, Lili et al. (2018) Potentially Functional Variants of ATG16L2 Predict Radiation Pneumonitis and Outcomes in Patients with Non-Small Cell Lung Cancer after Definitive Radiotherapy. J Thorac Oncol 13:660-675
Li, Bo; Wang, Yanru; Xu, Yinghui et al. (2018) Genetic variants in RORA and DNMT1 associated with cutaneous melanoma survival. Int J Cancer 142:2303-2312
Gearhart-Serna, Larisa M; Jayasundara, Nishad; Tacam Jr, Moises et al. (2018) Assessing Cancer Risk Associated with Aquatic Polycyclic Aromatic Hydrocarbon Pollution Reveals Dietary Routes of Exposure and Vulnerable Populations. J Environ Public Health 2018:5610462
Bakthavatsalam, Subha; Sleeper, Mark L; Dharani, Azim et al. (2018) Leveraging ?-Glutamyl Transferase To Direct Cytotoxicity of Copper Dithiocarbamates against Prostate Cancer Cells. Angew Chem Int Ed Engl 57:12780-12784

Showing the most recent 10 out of 513 publications