The DNA Microarray Facility provides access to various forms of technology for gene expression analysis, pairticularly DNA microarray technologies, to members ofthe Duke Comprehensive Caricer Institute. This includes Affymetrix microarrays as well as printed DNA microarrays produced in the facility. Importantly, the facility also provides service for microarray analysis, either with Affymetrix arrays or printed oligonucleotide arrays. As a result of the volume of use, the facility has negotiated an agreement with Affymetrix that now provides a very substantial discount in cost of microarrays and services, to the benefit of Cancer Institute members. Beyond the microarray services, the facility also provides Cancer Institute members access to an ABI 7900 RT-PCR instrument for validafion of gene expression results as well as alternative methods of expression assay, and access to an Axon Genepix Scanner for those wanting to scan their own arrays. In addifion, the facility provides substantial support for data analysis and management of microarray datasets. These services include inifial consultafion in experimental design, assessment of RNA quality, generafion of probes, hybridization to arrays, scanning of arrays, data analysis, and data management and storage. The Microarray Core Facility developed and uses a custom LIMS that integrates every point ofthe data production process, starting with sample submissions from researchers to laboratory operations, and then to final billing and posfings. With the LIMS tools and the repository features ofthe Express data repository, data of different types can be grouped and managed by projects, and because ofthe simplicity ofthe web-based interfaces that have been developed, researchers can execute their own analysis and data management in a simplified fashion. The impact of the facility on research in the Cancer Institute has been substantial, as evidenced by the large number of publications as well as the translation of these services into clinical practice in the form of multiple investigator-initiated clinical trials making use of DNA microarray analysis to guide clinical decisions.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA014236-38
Application #
8379553
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2012-01-01
Budget End
2012-12-31
Support Year
38
Fiscal Year
2012
Total Cost
$184,291
Indirect Cost
Name
Duke University
Department
Type
DUNS #
044387793
City
Durham
State
NC
Country
United States
Zip Code
27705
Xu, Yinghui; Wang, Yanru; Liu, Hongliang et al. (2018) Genetic variants in the metzincin metallopeptidase family genes predict melanoma survival. Mol Carcinog 57:22-31
Abdi, Khadar; Kuo, Chay T (2018) Laminating the mammalian cortex during development: cell polarity protein function and Hippo signaling. Genes Dev 32:740-741
Lu, Min; Sanderson, Sydney M; Zessin, Amelia et al. (2018) Exercise inhibits tumor growth and central carbon metabolism in patient-derived xenograft models of colorectal cancer. Cancer Metab 6:14
Qian, Danwen; Liu, Hongliang; Wang, Xiaomeng et al. (2018) Potentially functional genetic variants in the complement-related immunity gene-set are associated with non-small cell lung cancer survival. Int J Cancer :
Ashcraft, Kathleen A; Choudhury, Kingshuk Roy; Birer, Sam R et al. (2018) Application of a Novel Murine Ear Vein Model to Evaluate the Effects of a Vascular Radioprotectant on Radiation-Induced Vascular Permeability and Leukocyte Adhesion. Radiat Res 190:12-21
Ong, Cecilia T; Campbell, Brittany M; Thomas, Samantha M et al. (2018) Metaplastic Breast Cancer Treatment and Outcomes in 2500 Patients: A Retrospective Analysis of a National Oncology Database. Ann Surg Oncol 25:2249-2260
Duan, Bensong; Hu, Jiangfeng; Liu, Hongliang et al. (2018) Genetic variants in the platelet-derived growth factor subunit B gene associated with pancreatic cancer risk. Int J Cancer 142:1322-1331
Wu, Mengxi; Huang, Po-Hsun; Zhang, Rui et al. (2018) Circulating Tumor Cell Phenotyping via High-Throughput Acoustic Separation. Small 14:e1801131
Vlahovic, Gordana; Meadows, Kellen L; Hatch, Ace J et al. (2018) A Phase I Trial of the IGF-1R Antibody Ganitumab (AMG 479) in Combination with Everolimus (RAD001) and Panitumumab in Patients with Advanced Cancer. Oncologist 23:782-790
Xu, Yinghui; Liu, Hongliang; Liu, Shun et al. (2018) Genetic variant of IRAK2 in the toll-like receptor signaling pathway and survival of non-small cell lung cancer. Int J Cancer 143:2400-2408

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