? BIOREPOSITORY & PRECISION PATHOLOGY CENTER SHARED RESOURCE The BioRepository & Precision Pathology Center (BRPC) provides patient tissue and blood-based research support for the Duke Cancer Institute (DCI). Major service areas are Biobanking (BRPC broad consent, tissue, blood and fluid sample procurement, processing, storage and distribution including for the BRPC?s patient- derived xenograft (PDX) and primary cell culture (PCC) service lines, tumor enrichment through macro/microdissection, DNA/RNA extraction), Regulatory (protocol review, scope of work creation, budgeting, integration with broad consent if possible), and ImmunoHistology (traditional histology, single and multiplexed immunohistochemistry, in situ hybridization, tissue microarray creation, whole slide imaging and image analysis). Regarding the Biobanking service, the BRPC administers Duke?s largest broad consent protocol for specimen collection for research, which is becoming the backbone consent for new microbiome initiatives and has consented >4,700 patients since 2012. Of these patients, 17% are African American and 4% other non- Caucasian, supporting research into cancers of underserved minorities. The BRPC also operates a College of American Pathologists? (CAP)-accredited biorepository of normal and diseased samples representing a range of solid and hematolymphoid pathologies. Banking activities occur in conjunction with blood draws, standard-of- care biopsy and surgical procedures and clinical trial-supported tissue acquisitions. BRPC prioritizes activities based on instructions of DCI?s disease-site group leaders. Often a single biobanking event can simultaneously supply multiple ongoing research efforts, prepare a PDX model, prepare PCCs, and stock the biobank for future needs. BRPC biospecimens also are being used in Phase III of the NCI?s CPTAC project. The BRPC also serves as the administrative and regulatory backbone for the DCI?s Personalized Cancer Medicine Initiative (PCMI), which links genomic information, clinical annotation, and biospecimens. The gateway to the paraffin tissue archives in the Department of Pathology, BRPC provides regulatory support, cohort identification, and pathologist review for retrospective studies using those archives. BRPC rapidly retrieves and prepares patients? paraffin archival tissue samples for submission to clinical trial sponsors. Finally, the BRPC provides specialized tissue processing and experimental services including basic histology, veterinary pathology support for animal models, immunohistochemistry, in situ hybridization assays, tissue microarray creation, tumor enrichment by microdissection and laser capture microdissection, whole slide imaging and image analysis algorithms. BRPC biospecimens contributed to the Cancer Genome Atlas Project have supported numerous high-impact publications in Cell, Nature, Cancer Cell, and Cell Reports. In 2018, the BRPC provided services to 177 investigators, 58% of whom were DCI members who accounted for 92% of total usage and represented all 8 DCI Research Programs. Use of BRPC by DCI Members, contributed to 170 publications over the current project period, of which 54 were in high impact journals.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA014236-46
Application #
9853589
Study Section
Subcommittee I - Transistion to Independence (NCI)
Project Start
Project End
Budget Start
2020-01-01
Budget End
2020-12-31
Support Year
46
Fiscal Year
2020
Total Cost
Indirect Cost
Name
Duke University
Department
Type
DUNS #
044387793
City
Durham
State
NC
Country
United States
Zip Code
27705
Dai, Ziwei; Mentch, Samantha J; Gao, Xia et al. (2018) Methionine metabolism influences genomic architecture and gene expression through H3K4me3 peak width. Nat Commun 9:1955
Powell Gray, Bethany; Kelly, Linsley; Ahrens, Douglas P et al. (2018) Tunable cytotoxic aptamer-drug conjugates for the treatment of prostate cancer. Proc Natl Acad Sci U S A 115:4761-4766
Abdi, Khadar; Lai, Chun-Hsiang; Paez-Gonzalez, Patricia et al. (2018) Uncovering inherent cellular plasticity of multiciliated ependyma leading to ventricular wall transformation and hydrocephalus. Nat Commun 9:1655
Hudson, Kathryn E; Rizzieri, David; Thomas, Samantha M et al. (2018) Dose-intense chemoimmunotherapy plus radioimmunotherapy in high-risk diffuse large B-cell lymphoma and mantle cell lymphoma: a phase II study. Br J Haematol :
Fayanju, Oluwadamilola M; Park, Ko Un; Lucci, Anthony (2018) Molecular Genomic Testing for Breast Cancer: Utility for Surgeons. Ann Surg Oncol 25:512-519
Porter, Laura S; Fish, Laura; Steinhauser, Karen (2018) Themes Addressed by Couples With Advanced Cancer During a Communication Skills Training Intervention. J Pain Symptom Manage 56:252-258
Káradóttir, Ragnhildur T; Kuo, Chay T (2018) Neuronal Activity-Dependent Control of Postnatal Neurogenesis and Gliogenesis. Annu Rev Neurosci 41:139-161
Han, Peng; Liu, Hongliang; Shi, Qiong et al. (2018) Associations between expression levels of nucleotide excision repair proteins in lymphoblastoid cells and risk of squamous cell carcinoma of the head and neck. Mol Carcinog 57:784-793
Xu, Yinghui; Wang, Yanru; Liu, Hongliang et al. (2018) Genetic variants in the metzincin metallopeptidase family genes predict melanoma survival. Mol Carcinog 57:22-31
Abdi, Khadar; Kuo, Chay T (2018) Laminating the mammalian cortex during development: cell polarity protein function and Hippo signaling. Genes Dev 32:740-741

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