- LIGHT MICROSCOPY SHARED RESOURCE The Light Microscopy Shared Resource (LM) provides state-of-the-art light microscopy technology for fixed and live cells and tissues, along with scientific and technical expertise to assist in experimental design and optimal image collection. The LM shared resource offers affordable and efficient access to over $8 million in microscopic imaging resources, including confocal and conventional fluorescence instruments, multi-photon, lightsheet and super-resolution to all members of the Duke Cancer Institute (DCI). Three dedicated, full-time PhD-level staff members provide training and full technical support for all the microscopy systems. DCI members receive special priority access to equipment, training sessions and staff assistance. Hands-on individualized training and assistance with analysis is provided upon request to DCI members in advance of other users. LM is supported annually with charge backs from user fees and by the DCI CCSG, the Duke University School of Medicine and the Duke University Office of the Provost. All standard optical sectioning and fluorescence microscopy technologies are offered including confocal, resonant scanning confocal, spinning disk, live-cell imaging with several modalities, long-term incubated time-lapse, multi-photon, photoactivation/photoconversion and photokinetic (FRAP, FLIP) capabilities, and in addition, new technologies ? lightsheet and super-resolution. Reservations are executed online through the university-wide software system on a first-come, first-served basis with some limits to promote efficient use. The large range of equipment with some replication of capabilities provides good capacity for users? needs. LM?s continued priority is to serve the light microscopy needs of DCI members and all Duke researchers with support and assistance on equipment and image analysis in order to generate productivity. At the same time we keep current by demo-ing and testing new and different technologies and strive to implement new technologies that prove valuable. In 2018, LM provided services to 233 investigators, 40% of whom were DCI members, accounting for 55% of total usage, from all 8 DCI Research Programs. Use of this shared resource by DCI Members contributed to 261 publications over the project period, 71 of which were in high impact journals (IF>9), demonstrating the value of services offered by the resource.

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National Cancer Institute (NCI)
Center Core Grants (P30)
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Subcommittee I - Transistion to Independence (NCI)
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Duke University
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Xu, Yinghui; Wang, Yanru; Liu, Hongliang et al. (2018) Genetic variants in the metzincin metallopeptidase family genes predict melanoma survival. Mol Carcinog 57:22-31
Abdi, Khadar; Kuo, Chay T (2018) Laminating the mammalian cortex during development: cell polarity protein function and Hippo signaling. Genes Dev 32:740-741
Lu, Min; Sanderson, Sydney M; Zessin, Amelia et al. (2018) Exercise inhibits tumor growth and central carbon metabolism in patient-derived xenograft models of colorectal cancer. Cancer Metab 6:14
Qian, Danwen; Liu, Hongliang; Wang, Xiaomeng et al. (2018) Potentially functional genetic variants in the complement-related immunity gene-set are associated with non-small cell lung cancer survival. Int J Cancer :
Ashcraft, Kathleen A; Choudhury, Kingshuk Roy; Birer, Sam R et al. (2018) Application of a Novel Murine Ear Vein Model to Evaluate the Effects of a Vascular Radioprotectant on Radiation-Induced Vascular Permeability and Leukocyte Adhesion. Radiat Res 190:12-21
Ong, Cecilia T; Campbell, Brittany M; Thomas, Samantha M et al. (2018) Metaplastic Breast Cancer Treatment and Outcomes in 2500 Patients: A Retrospective Analysis of a National Oncology Database. Ann Surg Oncol 25:2249-2260
Duan, Bensong; Hu, Jiangfeng; Liu, Hongliang et al. (2018) Genetic variants in the platelet-derived growth factor subunit B gene associated with pancreatic cancer risk. Int J Cancer 142:1322-1331
Wu, Mengxi; Huang, Po-Hsun; Zhang, Rui et al. (2018) Circulating Tumor Cell Phenotyping via High-Throughput Acoustic Separation. Small 14:e1801131
Vlahovic, Gordana; Meadows, Kellen L; Hatch, Ace J et al. (2018) A Phase I Trial of the IGF-1R Antibody Ganitumab (AMG 479) in Combination with Everolimus (RAD001) and Panitumumab in Patients with Advanced Cancer. Oncologist 23:782-790
Xu, Yinghui; Liu, Hongliang; Liu, Shun et al. (2018) Genetic variant of IRAK2 in the toll-like receptor signaling pathway and survival of non-small cell lung cancer. Int J Cancer 143:2400-2408

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