The Flow Cytometry Laboratory (Flow Lab) was established by the University of Wisconsin Carbone Cancer Center (UWCCC) in 1988 to support the laboratory, translational and clinical research activities of our members. The mission of the Flow Lab is to provide state-of-the-art instrumentation, technical expertise, cutting edge and cost-effective services and high quality education programs to advance laboratory, translational and clinical cancer research through the use of flow cytometry and high content cell imaging. The Flow Lab currently provides services at two sites. The main laboratory is located at the Wisconsin Institutes for Medical Research (WIMR), on the west side of the UW-Madison campus and a satellite laboratory is operating at the McArdle Laboratory for Cancer Research (McArdle), on the central UW Madison campus. The Flow Lab maintains four benchtop flow cytometer analysis instruments, two high-speed sorting cytometers, a high-throughput bioimaging microscope, as well as a fluorescent microscope with camera and supporting instrumentation such as biosafety cabinets, centrifuges and incubators. The Flow Lab is staffed from 8;00am to 6;00pm Monday through Friday and instrumentation is available to trained users at all times. Through support from the Cancer Center Support Grant (CCSG), successful instrumentation grants, institutional funds and charges to users, the Flow Lab is able to provide the instrumentation and expertise needed to serve the diverse research needs of UWCCC members in areas relating to use of analytical flow cytometry, cell sorting and high content cell imaging.

Public Health Relevance

The mission of the Flow Cytometry Laboratory is to provide state-of-the-art instruments, technical expertise, cutting edge services and high quality education programs to advance laboratory, translational and clinical cancer research through the use of flow cytometry and high content cell imaging.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA014520-41
Application #
8835039
Study Section
Subcommittee I - Transistion to Independence (NCI)
Project Start
Project End
2016-03-31
Budget Start
2015-04-01
Budget End
2016-03-31
Support Year
41
Fiscal Year
2015
Total Cost
Indirect Cost
Name
University of Wisconsin Madison
Department
Type
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715
Esbona, Karla; Yi, Yanyao; Saha, Sandeep et al. (2018) The Presence of Cyclooxygenase 2, Tumor-Associated Macrophages, and Collagen Alignment as Prognostic Markers for Invasive Breast Carcinoma Patients. Am J Pathol 188:559-573
Hernández-Santos, Nydiaris; Wiesner, Darin L; Fites, J Scott et al. (2018) Lung Epithelial Cells Coordinate Innate Lymphocytes and Immunity against Pulmonary Fungal Infection. Cell Host Microbe 23:511-522.e5
van Ravesteyn, Nicolien T; van den Broek, Jeroen J; Li, Xiaoxue et al. (2018) Modeling Ductal Carcinoma In Situ (DCIS): An Overview of CISNET Model Approaches. Med Decis Making 38:126S-139S
Robins, H Ian; Nguyen, HuyTram N; Field, Aaron et al. (2018) Molecular Evolution of a Glioblastoma Controlled With Tumor Treating Fields and Concomitant Temozolomide. Front Oncol 8:451
Pezzi, Hannah M; Niles, David J; Schehr, Jennifer L et al. (2018) Integration of Magnetic Bead-Based Cell Selection into Complex Isolations. ACS Omega 3:3908-3917
Alagoz, Oguzhan; Ergun, Mehmet Ali; Cevik, Mucahit et al. (2018) The University of Wisconsin Breast Cancer Epidemiology Simulation Model: An Update. Med Decis Making 38:99S-111S
Lyu, Zhigang; Kang, Lei; Buuh, Zakey Yusuf et al. (2018) A Switchable Site-Specific Antibody Conjugate. ACS Chem Biol 13:958-964
Hardin, Heather; Helein, Holly; Meyer, Kristy et al. (2018) Thyroid cancer stem-like cell exosomes: regulation of EMT via transfer of lncRNAs. Lab Invest 98:1133-1142
Xu, Cheng; Chen, Feng; Valdovinos, Hector F et al. (2018) Bacteria-like mesoporous silica-coated gold nanorods for positron emission tomography and photoacoustic imaging-guided chemo-photothermal combined therapy. Biomaterials 165:56-65
Wargowski, Ellen; Johnson, Laura E; Eickhoff, Jens C et al. (2018) Prime-boost vaccination targeting prostatic acid phosphatase (PAP) in patients with metastatic castration-resistant prostate cancer (mCRPC) using Sipuleucel-T and a DNA vaccine. J Immunother Cancer 6:21

Showing the most recent 10 out of 1528 publications