The objective of the Oligopeptide Facility is to provide primary structural data on various proteins and peptides under study by members of the Cancer Center and other faculty of the University of Chicago and outside. Services that are provided by the facility include amino acid analysis or composition of proteins, amino-terminal amino acid sequence analysis of proteins/peptides, and the production of synthetic peptides. In addition, the facility provides individualized consultation to Cancer Center researchers to enable them to design, synthesize, sequence and analyze peptides, and workshops for all interested staff and faculty. The peptide microsequencing capability of this Core provides CRC investigators with amino acid sequence data from extremely small quantities of protein for the purposes of identification of the protein product where the sequence is known, comparison with protein sequence information for determination of similarity and generating synthetic peptides for further biological and molecular characterization of these proteins. The peptide synthetic capability of the core provides CRC investigators with the ability to investigate peptides of interest to them through generation of antibodies, isotope mapping or assessment of biological properties. This facility was opened in March, 1988. Thirty-four Cancer Center investigators are users of the facility. The apparent need is mostly for peptides to produce antibodies or epitope mapping. The Core attempts to remain responsive to the needs of the Cancer Center members, determines priority of services, and keeps Center members informed of new developments and technical advances.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA014599-27
Application #
6397831
Study Section
Project Start
2000-08-01
Project End
2002-03-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
27
Fiscal Year
2000
Total Cost
$289,716
Indirect Cost
Name
University of Chicago
Department
Type
DUNS #
225410919
City
Chicago
State
IL
Country
United States
Zip Code
60637
Trujillo, Jonathan A; Sweis, Randy F; Bao, Riyue et al. (2018) T Cell-Inflamed versus Non-T Cell-Inflamed Tumors: A Conceptual Framework for Cancer Immunotherapy Drug Development and Combination Therapy Selection. Cancer Immunol Res 6:990-1000
Zeng, Zongyue; Huang, Bo; Huang, Shifeng et al. (2018) The development of a sensitive fluorescent protein-based transcript reporter for high throughput screening of negative modulators of lncRNAs. Genes Dis 5:62-74
Lee, Ji-Hye; Park, Beom Seok; Han, Kang R et al. (2018) Insight Into the Interaction Between RNA Polymerase and VPg for Murine Norovirus Replication. Front Microbiol 9:1466
Cheng, Jason X; Chen, Li; Li, Yuan et al. (2018) RNA cytosine methylation and methyltransferases mediate chromatin organization and 5-azacytidine response and resistance in leukaemia. Nat Commun 9:1163
Johnson, Marianna B; Hoffmann, Joscelyn N; You, Hannah M et al. (2018) Psychosocial Stress Exposure Disrupts Mammary Gland Development. J Mammary Gland Biol Neoplasia 23:59-73
Sweis, Randy F; Zha, Yuanyuan; Pass, Lomax et al. (2018) Pseudoprogression manifesting as recurrent ascites with anti-PD-1 immunotherapy in urothelial bladder cancer. J Immunother Cancer 6:24
Kathayat, Rahul S; Cao, Yang; Elvira, Pablo D et al. (2018) Active and dynamic mitochondrial S-depalmitoylation revealed by targeted fluorescent probes. Nat Commun 9:334
Liu, Jun; Eckert, Mark A; Harada, Bryan T et al. (2018) m6A mRNA methylation regulates AKT activity to promote the proliferation and tumorigenicity of endometrial cancer. Nat Cell Biol 20:1074-1083
Bhanvadia, Raj R; VanOpstall, Calvin; Brechka, Hannah et al. (2018) MEIS1 and MEIS2 Expression and Prostate Cancer Progression: A Role For HOXB13 Binding Partners in Metastatic Disease. Clin Cancer Res 24:3668-3680
Wood, Kevin; Byron, Elizabeth; Janisch, Linda et al. (2018) Capecitabine and Celecoxib as a Promising Therapy for Thymic Neoplasms. Am J Clin Oncol 41:963-966

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