The Flow Cytometry Shared Resource (FCSR) provides state-of-the-art analytical and sorting instrumentation as well as cutting-edge expertise, to UCLA investigators needing flow cytometry for their research. The Jonsson Comprehensive Cancer Center (JCCC) established this shared resource in 1988. Beth Jamieson (Tl), has been the director of the FCSR since 2000. Ms. Ingrid Schmid, the manager of the FCSR since 1988, is responsible for the daily operation of the FCSR and assists Jamieson (Tl) with long-term planning. Both Jamieson (Tl) and Schmid are available to users for consultation. To achieve its goals, the FCSR houses five analytic flow cytometers, three high-speed FACSAria cell sorters, a RoboSep magnetic cell separator and a freestanding analysis station. The FCSR is easily accessible and is responsible for procurement, daily calibration, maintenance and repair of all instrumentation. FCSR personnel have a combined 98 years of experience in flow cytometry;their expertise is available to investigators through one-on-one consultation, frequent classes, a website, poster presentations and scheduled open houses. Schmid and Jamieson (Tl) also work with researchers to adapt or develop assays to enable investigators to pursue research questions in novel ways. FCSR services help researchers with a widely varied range of experience in flow cytometry to perform high quality assays with minimal time expenditure. Workshops, seminars and implementation of new assays ensure that researchers remain on the cutting-edge of flow cytometry without the necessity of investing their own funds or staff time to keep current in the latest advances. Cytometric instrumentation is expensive and beyond the budget of most individual researchers. Therefore, the FCSR provides instrumentation, as well as additional cost savings to researchers, by obtaining grant and institutional support to help offset the cost of purchasing, maintaining and operating those instruments. From 07/01/2011 to 06/30/2012, the FCSR had 170 users, 110 of whom were JCCC members, representing eight Cancer Center Program Areas. Of the 110 member users, 95 had peer-reviewed funding and 15 did not have peer-reviewed funding.

Public Health Relevance

): Preliminary data analysis by the CDC revealed that in 2010, neoplasms remained the second leading cause of death within the United States. The FCSR supports the work of JCCC investigators who strive to understand all aspects of cancer, ranging from its causes to its prevention and treatment. By supplying expertise and instrumentation that allow JCCC investigators to address novel research and clinical questions, the FCSR serves as a critical resource in the fight against cancer.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA016042-39
Application #
8635439
Study Section
Subcommittee G - Education (NCI)
Project Start
1996-12-01
Project End
2018-11-30
Budget Start
2014-03-07
Budget End
2014-11-30
Support Year
39
Fiscal Year
2014
Total Cost
$163,740
Indirect Cost
Name
University of California Los Angeles
Department
Type
DUNS #
092530369
City
Los Angeles
State
CA
Country
United States
Zip Code
90095
Lisova, Ksenia; Sergeev, Maxim; Evans-Axelsson, Susan et al. (2018) Microscale radiosynthesis, preclinical imaging and dosimetry study of [18F]AMBF3-TATE: A potential PET tracer for clinical imaging of somatostatin receptors. Nucl Med Biol 61:36-44
Chang, Yu-Ling; Rossetti, Maura; Vlamakis, Hera et al. (2018) A screen of Crohn's disease-associated microbial metabolites identifies ascorbate as a novel metabolic inhibitor of activated human T cells. Mucosal Immunol :
Kiertscher, Sylvia M; Gangalum, Pallavi R; Ibrahim, Grace et al. (2018) A Prospective Study of Humoral and Cellular Immune Responses to Hepatitis B Vaccination in Habitual Marijuana Smokers. J Neuroimmune Pharmacol 13:219-229
Jia, Qingmei; Bowen, Richard; Dillon, Barbara Jane et al. (2018) Single vector platform vaccine protects against lethal respiratory challenge with Tier 1 select agents of anthrax, plague, and tularemia. Sci Rep 8:7009
Van, Christina; Condro, Michael C; Lov, Kenny et al. (2018) PACAP/PAC1 Regulation of Inflammation via Catecholaminergic Neurons in a Model of Multiple Sclerosis. J Mol Neurosci :
Leoh, Lai Sum; Kim, Yoon Kyung; Candelaria, Pierre V et al. (2018) Efficacy and Mechanism of Antitumor Activity of an Antibody Targeting Transferrin Receptor 1 in Mouse Models of Human Multiple Myeloma. J Immunol 200:3485-3494
Hicks, Michael R; Hiserodt, Julia; Paras, Katrina et al. (2018) ERBB3 and NGFR mark a distinct skeletal muscle progenitor cell in human development and hPSCs. Nat Cell Biol 20:46-57
Tsang, Eric J; Wu, Benjamin; Zuk, Patricia (2018) MAPK signaling has stage-dependent osteogenic effects on human adipose-derived stem cells in vitro. Connect Tissue Res 59:129-146
Waters, Lynnea R; Ahsan, Fasih M; Wolf, Dane M et al. (2018) Initial B Cell Activation Induces Metabolic Reprogramming and Mitochondrial Remodeling. iScience 5:99-109
Van Dyk, Kathleen; Bower, Julienne E; Crespi, Catherine M et al. (2018) Cognitive function following breast cancer treatment and associations with concurrent symptoms. NPJ Breast Cancer 4:25

Showing the most recent 10 out of 767 publications