The Flow and Image Cytometry Resource provides advanced flow cytometric and morphologyservice at the light and electron microscope (EM) levels of resolution utilizing state-of-the-art technology andan extensive computer network that has been assembled into a user-friendly environment. The Resource issupervised by Paul K. Wallace, PhD, and Hans Minderman, PhD, and is staffed by five technical personnel.The goal is to provide (i) multiparameter flow cytometry, encompassing analytical, sorting and Luminexservices, (ii) morphological services at the light and EM levels of resolution, and (iii) instruction and technicalsupport as required by investigators. As a focal point for many interdisciplinary activities throughout RPCI,the Resource supports both basic research and clinical protocol services. The Resource has an extensiveimmunophenotyping service and is the core flow cytometric laboratory for many NIH-initiated national clinicaltrials.The Flow Cytometry Component of the Resource offers both clinical and basic research supportservices, which universally maintain a quality assurance program using guidelines from state and nationalaccrediting agencies. The Imaging Component enables qualitative and quantitative image analysis on thetissue, cellular and subcellular levels, time-kinetic multicolor image analysis and quantitative multispectralimage analysis. The Educational Program Component provides didactic lectures and hands-on experiencewith (i) isolation, preparation, and staining of all types of human and animal cells, (ii) instrument setup andacquisition, and (iii) data analysis,The Resource has complete general biology, tissue culture, optics and electronics, and computerlaboratories, as well as a fabrication shop. The Resource's strategic plan emphasizes the provision of stateof-the-art technology and expertise in all facets of flow and image cytometry, and supporting the needs ofCCSG Program members to enhance peer-reviewed funding and publications.During the reporting period (4/1/06 to 3/31/07), the Resource contributed to the research of 67investigators from all six CCSG Programs, with 85% of Resource utilization by CCSG Program memberswith peer-reviewed funding. $110,245 in CCSG support is requested, representing 8% of the total operatingbudget.
Showing the most recent 10 out of 1555 publications
