The Flow and Image Cytometry Shared Resource (FICR) provides advanced flow cytometric and morphology service utilizing state-of-the-art technology and an extensive computer network that has been assembled into a user-friendly and cost-effective environment. The Resource is supervised by Paul K. Wallace, PhD, and Hans Minderman, PhD, and is staffed by an additional 10 technical personnel with responsibilities for operating and maintaining the equipment and assisting users with their flow cytometric and imaging experiments. The goals are to provide (i) multiparameter flow cytometry, encompassing analytical, sorting and Luminex services, (ii) Confocal, Live Cell and ImageStream imaging services, and (iii) instruction and technical support as required by investigators. As a focal point for many interdisciplinary activities throughout the cancer center, the Resource supports both basic research and clinical protocol services. The Resource's strategic plan emphasizes the provision of state-of-the-art technology and expertise in all facets of flow and image cytometry, and supporting the needs of CCSG Program members to enhance peer-reviewed funding and publications. The flow cytometry component of the Resource offers both clinical and basic research support services, which universally maintain a quality assurance program using guidelines from NY State and national accrediting agencies. The imaging component enables qualitative and quantitative image analysis of cells at the cellular and subcellular levels, time-kinetic multicolor image analysis and quantitative multispectral image analysis. The educational program component provides didactic lectures and hands-on experience with (i) isolation, preparation, and staining of all types of human and animal cells, (ii) instrument setup and acquisition, and (iii) data analysis. First priority for use is given to peer-review-funded CCSG members;second priority to non-peer-review-funded CCSG members;third priority to non-members and academic collaborators;and last priority to external users. During the reporting period, the Flow and Image Cytometry Shared Resource has served 75 members from 6 research programs, with 40% utilization by CCSG members with peer reviewed funding. The CCSG support provides 6% of the overall proposed budget.

Public Health Relevance

The ability to analyze the expression of molecules on the surface and within individual cells, and to sort those cells based on expression of those molecules, is an essential tool for many cancer investigators. The cancer research supported by this shared resource provides state-of-the-art facilities and outstanding expertise in flow cytometry and cell sorting.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA016056-37
Application #
8738369
Study Section
Subcommittee G - Education (NCI)
Project Start
1997-06-16
Project End
2019-04-30
Budget Start
2014-06-26
Budget End
2015-04-30
Support Year
37
Fiscal Year
2014
Total Cost
$149,257
Indirect Cost
$59,037
Name
Roswell Park Cancer Institute Corp
Department
Type
DUNS #
824771034
City
Buffalo
State
NY
Country
United States
Zip Code
14263
Mayor, Paul C; Eng, Kevin H; Singel, Kelly L et al. (2018) Cancer in primary immunodeficiency diseases: Cancer incidence in the United States Immune Deficiency Network Registry. J Allergy Clin Immunol 141:1028-1035
Dasgupta, Subhamoy; Rajapakshe, Kimal; Zhu, Bokai et al. (2018) Metabolic enzyme PFKFB4 activates transcriptional coactivator SRC-3 to drive breast cancer. Nature 556:249-254
La Shu, Shin; Yang, Yunchen; Allen, Cheryl L et al. (2018) Metabolic reprogramming of stromal fibroblasts by melanoma exosome microRNA favours a pre-metastatic microenvironment. Sci Rep 8:12905
Zhang, Dingxiao; Tang, Dean G; Rycaj, Kiera (2018) Cancer stem cells: Regulation programs, immunological properties and immunotherapy. Semin Cancer Biol 52:94-106
Gabriel, Emmanuel; Attwood, Kristopher; Al-Sukhni, Eisar et al. (2018) Age-related rates of colorectal cancer and the factors associated with overall survival. J Gastrointest Oncol 9:96-110
Ma, Wen Wee; Xie, Hao; Fetterly, Gerald et al. (2018) A Phase Ib Study of the FGFR/VEGFR Inhibitor Dovitinib With Gemcitabine and Capecitabine in Advanced Solid Tumor and Pancreatic Cancer Patients. Am J Clin Oncol :
Chen, George L; Carpenter, Paul A; Broady, Raewyn et al. (2018) Anti-Platelet-Derived Growth Factor Receptor Alpha Chain Antibodies Predict for Response to Nilotinib in Steroid-Refractory or -Dependent Chronic Graft-Versus-Host Disease. Biol Blood Marrow Transplant 24:373-380
Eng, Kevin H; Szender, J Brian; Etter, John Lewis et al. (2018) Paternal lineage early onset hereditary ovarian cancers: A Familial Ovarian Cancer Registry study. PLoS Genet 14:e1007194
Barger, Carter J; Zhang, Wa; Sharma, Ashok et al. (2018) Expression of the POTE gene family in human ovarian cancer. Sci Rep 8:17136
Bucsek, Mark J; Giridharan, Thejaswini; MacDonald, Cameron R et al. (2018) An overview of the role of sympathetic regulation of immune responses in infectious disease and autoimmunity. Int J Hyperthermia 34:135-143

Showing the most recent 10 out of 1555 publications