? FLOW CYTOMETRY SHARED RESOURCE (FCSR) Flow cytometry services are critical for cancer research. Since flow cytometry instrumentation is expensive, and typically requires significant technical expertise, a centralized resource is essential. The mission of the FCSR is to support OSUCCC members and high-impact cancer research projects with high quality cell analysis and sorting capabilities. The FCSR also provides training and regularly host technology-based seminars or workshops to introduce new technology to OSUCCC members. The FCSR continues to strive to be a state-of- the-art cell analysis laboratory that has the following Specific Aims to: 1) provide state-of-the-art equipment and support for high-quality cancer research for OSUCCC members on a fee-for-service basis; 2) continuously work with OSUCCC members to provide substantial technical expertise and training for state-of the-art cytometry instruments to address fundamental questions in cancer research so that researchers can have 24-hour access to flow cytometry instrumentation; and 3) introduce OSUCCC members to new instrumentation, technology and methodologies being developed at the FCSR through a variety of educational outreach activities. The FCSR co- Directors are Jeffrey Chalmers, (Department of Chemical and Biomolecular Engineering), and recently appointed co-Director, Kevin Weller, Associate Director of the Pelotonia Institute of Immuno-Oncology (PIIO) and specifically to develop the Immune Monitoring and Discovery Platform, a cross-cutting shared-resource initiative that integrates shared resources for complex immuno-oncology projects. Further, Dr. Gregory Behbehani (LR) is a Faculty Advisor for the Helios mass cytometer (CyTOF) system. Over the current grant cycle, major changes to the FCSR were to align with the establishment of the PIIO, which will result in a substantial increase in the need for flow cytometry services. To address this need, new instrumentation was purchased including four new instruments including a state-of-the-art Cytek Aurora flow cytometer and a Helios mass cytometer. During this time period, the FCSR contributed to 231 publications (39 >10 impact factor), had 296 users with 34,118 hours of service (83.7% to OSUCCC members), and supported 80 NCI grants (1 K12, 1 K22, 1 K24, 6 P01s, 1 P50, 52 R01s, 1 R03, 7 R21s, 3 R35s, 1 R37, 1 T32, and 5 U01s). In the next funding cycle, the FCSR will support the increasing needs of all OSUCCC strategic priorities, including immuno-oncology, translational genomics, cancer engineering, and cancer prevention and survivorship. The user base will substantially increased requiring new staff and technologies, for example by purchasing an additional Aurora instrument, and increasing cell sorting, cell isolation, and exosome/microvesicle research. In addition, new services under development include development of further exosome characterization and isolation technology. The annual budget of the FCSR is $518,075, yet the CCSG request is $108,261. As such, the FCSR leverages extensive institutional support and seeks only 20.9% support from CCSG funds.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
Project #
Application #
Study Section
Subcommittee H - Clinical Groups (NCI)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Ohio State University
United States
Zip Code
Fu, Xinping; Tao, Lihua; Wang, Pin-Yi et al. (2018) Comparison of infectivity and spread between HSV-1 and HSV-2 based oncolytic viruses on tumor cells with different receptor expression profiles. Oncotarget 9:21348-21358
Brewington, Beatrice Y; Shao, Yusra F; Davidorf, Fredrick H et al. (2018) Brachytherapy for patients with uveal melanoma: historical perspectives and future treatment directions. Clin Ophthalmol 12:925-934
Doogan, Nathan J; Cooper, Sarah; Quisenberry, Amanda J et al. (2018) The role of travel distance and price promotions in tobacco product purchase quantity. Health Place 51:151-157
Byrd, John C; Ruppert, Amy S; Heerema, Nyla A et al. (2018) Lenalidomide consolidation benefits patients with CLL receiving chemoimmunotherapy: results for CALGB 10404 (Alliance). Blood Adv 2:1705-1718
Oblinger, Janet L; Burns, Sarah S; Huang, Jie et al. (2018) Overexpression of eIF4F components in meningiomas and suppression of meningioma cell growth by inhibiting translation initiation. Exp Neurol 299:299-307
Shu, Yi; Yin, Hongran; Rajabi, Mehdi et al. (2018) RNA-based micelles: A novel platform for paclitaxel loading and delivery. J Control Release 276:17-29
Scoville, Steven D; Nalin, Ansel P; Chen, Luxi et al. (2018) Human AML activates the aryl hydrocarbon receptor pathway to impair NK cell development and function. Blood 132:1792-1804
McMillan, Elizabeth A; Ryu, Myung-Jeom; Diep, Caroline H et al. (2018) Chemistry-First Approach for Nomination of Personalized Treatment in Lung Cancer. Cell 173:864-878.e29
Schimizzi, Gregory V; Jin, Linda X; Davidson 4th, Jesse T et al. (2018) Outcomes after vascular resection during curative-intent resection for hilar cholangiocarcinoma: a multi-institution study from the US extrahepatic biliary malignancy consortium. HPB (Oxford) 20:332-339
Wang, Yufeng; Dong, Wenjuan; Zhang, Yibo et al. (2018) Dependence of innate lymphoid cell 1 development on NKp46. PLoS Biol 16:e2004867

Showing the most recent 10 out of 2602 publications