The Flow Cytometry shared resource of the Virginia Commonwealth (VCU) Massey Cancer Center (MCC) was founded in 1976. Its instrumentation has been regularly upgraded through eight generations of flow cytometers to the five flow instruments currently in use, two multi-laser BD Aria II sorters, one of which is located in a Baker BioProtect Hood, three analyzers, a BD Canto II, a Coulter FC500 and a Coulter XL-MCL. Capabilities include up to nine color simultaneous detection and up to four simultaneous sorts per Aria II. The Flow Cytometry shared resource also has a Biacore T100 instrument for surface plasmon resonance (SPR) studies. The Director, Dr Daniel H. Conrad, has over 25 years of experience in flow cytometry and over 12 years of experience with SPR technology. Dr Conrad, whose office and laboratory are in close proximity to the instruments in the Kontos Medical Science Building (KMSB), meets regularly with core personnel and oversees planning for the new facility. The Resource Manager, Ms Julie Farnsworth, is highly trained in flow cytometry and has been with the Flow Cytometry shared resource for nine years. Previous to this, she worked for the Flow Cytometry core at the University of North Carolina (UNC) for two years. The resource provides a wide range of services, from routine fluorescence analysis to interactive custom design of innovative analysis and sorting protocols, addressing the specific needs of individual investigators. Dr Conrad closely follows the analyses performed by the core facility, insuring quality control, and advising facility users on alternative, often novel, approaches to experimental design and data analysis. Since the last review, the resource has upgraded facilities and instrumentation, moved to a new central location and added the capabilities for multiple high-speed and biohazardous sorting. This shared resource maintains a stable base of instrumentation, expertise and trained personnel and currently provides services and training for multiple users in 69 research groups from 21 departments at VCU. It has had a major impact on cancer research at the VCU MCC for 30 years.
Flow cytometry has important applications in basic, translational, and clinical cancer research. Flow cytometry is used as a tool to measure molecules both on and in individual cells. This capacity gives cancer researchers the ability to identify the differences between normal and cancerous cells, to quantify the immune response to cancer cells, and to measure the response of cancer cells to treatment.
|Singh, Dhirendra P; Kaur, Gagandeep; Bagam, Prathyusha et al. (2018) Membrane microdomains regulate NLRP10- and NLRP12-dependent signalling in A549 cells challenged with cigarette smoke extract. Arch Toxicol 92:1767-1783|
|Zhang, Yong; Liu, Hong; Li, Wei et al. (2018) Intraflagellar transporter protein 140 (IFT140), a component of IFT-A complex, is essential for male fertility and spermiogenesis in mice. Cytoskeleton (Hoboken) 75:70-84|
|Zhou, Liang; Zhang, Yu; Sampath, Deepak et al. (2018) Flavopiridol enhances ABT-199 sensitivity in unfavourable-risk multiple myeloma cells in vitro and in vivo. Br J Cancer 118:388-397|
|Lochmann, Timothy L; Floros, Konstantinos V; Naseri, Mitra et al. (2018) Venetoclax Is Effective in Small-Cell Lung Cancers with High BCL-2 Expression. Clin Cancer Res 24:360-369|
|Salman, Ali; Koparde, Vishal; Hall, Charles E et al. (2018) Determining the Quantitative Principles of T Cell Response to Antigenic Disparity in Stem Cell Transplantation. Front Immunol 9:2284|
|Huang, Dian; Leslie, Kevin A; Guest, Daniel et al. (2018) High-Speed Live-Cell Interferometry: A New Method for Quantifying Tumor Drug Resistance and Heterogeneity. Anal Chem 90:3299-3306|
|Maczis, Melissa A; Maceyka, Michael; Waters, Michael R et al. (2018) Sphingosine kinase 1 activation by estrogen receptor ?36 contributes to tamoxifen resistance in breast cancer. J Lipid Res 59:2297-2307|
|Deng, Yongqiang; Pakdel, Mehrshad; Blank, Birgit et al. (2018) Activity of the SPCA1 Calcium Pump Couples Sphingomyelin Synthesis to Sorting of Secretory Proteins in the Trans-Golgi Network. Dev Cell 47:464-478.e8|
|Nulton, Tara J; Kim, Nak-Kyeong; DiNardo, Laurence J et al. (2018) Patients with integrated HPV16 in head and neck cancer show poor survival. Oral Oncol 80:52-55|
|Porter-Stransky, Kirsten A; Centanni, Samuel W; Karne, Saumya L et al. (2018) Noradrenergic Transmission at Alpha1-Adrenergic Receptors in the Ventral Periaqueductal Gray Modulates Arousal. Biol Psychiatry :|
Showing the most recent 10 out of 586 publications