PROTEOMICS SHARED RESOURCE The Proteomics SR (PROT) is a state-of-the-art facility that brings cutting edge proteomics research to LCCC faculty. It is a well-established shared resource (SR) that provides consultation for the design of experiments, sample preparation, mass spectrometry (MS) and extensive data analysis for LCCC members. Directed by an expert staff, the SR is full service allowing users with projects but little experience to understand the range of data acquisition opportunities. The SR also has the instrumentation that allows more experienced users access to expensive, well maintained mass spectrometers. Core staff and expert users have extended the capabilities with innovative techniques such as the MIB/MS analysis of cell wide kinase activity. Contributions include validating and characterizing cancer-relevant drug targets, quantifying proteome and signaling network changes in response to emerging therapeutics, studying the dynamic regulation of the kinome, describing how specific pathways globally remodel cancer proteomes independent of changes in gene expression, and determining how protein interaction networks are dysregulated in cancer. Instrumentation includes; state-of-the-art mass spectrometers include: Thermo Lumos, Thermo QExactive HF- X, Thermo QExactive Biopharma, Thermo QExactive HF, Thermo Orbitrap Velos and Sciex 5800 MALDI/TOF/TOF. All were partially purchased or leased by LCCC including four within the last four years. The purchase and maintenance of the current PROT SR instruments are beyond the means of any individual lab. LCCC investments have made it possible for multiple labs to access high end technology. The cost of PROT SR services are generally lower by 25% than comparable private company or other academic institutions and LCCC members receive an additional discount making the facility quite cost effective. The LCCC is requesting $135,301 from the CCSG, which represents 21% of the total operating budget; 48% of the SR usage is by LCCC faculty.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA016086-45
Application #
10089829
Study Section
Subcommittee I - Transistion to Independence (NCI)
Project Start
1997-06-01
Project End
2025-11-30
Budget Start
2020-12-01
Budget End
2021-11-30
Support Year
45
Fiscal Year
2021
Total Cost
Indirect Cost
Name
University of North Carolina Chapel Hill
Department
Type
DUNS #
608195277
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599
Mayer, Deborah K; Landucci, Gina; Awoyinka, Lola et al. (2018) SurvivorCHESS to increase physical activity in colon cancer survivors: can we get them moving? J Cancer Surviv 12:82-94
Huo, Dezheng; Perou, Charles M; Olopade, Olufunmilayo I (2018) Reported Biologic Differences in Breast Cancer by Race Due to Disparities in Screening-Reply. JAMA Oncol 4:883-884
Howe, Chanelle J; Robinson, Whitney R (2018) Survival-related Selection Bias in Studies of Racial Health Disparities: The Importance of the Target Population and Study Design. Epidemiology 29:521-524
Byrne, James D; Yeh, Jen Jen; DeSimone, Joseph M (2018) Use of iontophoresis for the treatment of cancer. J Control Release 284:144-151
Wilkin, Timothy J; Chen, Huichao; Cespedes, Michelle S et al. (2018) A Randomized, Placebo-Controlled Trial of the Quadrivalent Human Papillomavirus Vaccine in Human Immunodeficiency Virus-Infected Adults Aged 27 Years or Older: AIDS Clinical Trials Group Protocol A5298. Clin Infect Dis 67:1339-1346
Siegel, Marni B; He, Xiaping; Hoadley, Katherine A et al. (2018) Integrated RNA and DNA sequencing reveals early drivers of metastatic breast cancer. J Clin Invest 128:1371-1383
Ubil, Eric; Caskey, Laura; Holtzhausen, Alisha et al. (2018) Tumor-secreted Pros1 inhibits macrophage M1 polarization to reduce antitumor immune response. J Clin Invest 128:2356-2369
Hamad, Ahmad; Iweala, Onyinye I; Henderson, Cory et al. (2018) Recurrent anaphylaxis during cardiac catheterization due to ethylene oxide. J Allergy Clin Immunol Pract 6:2148-2150
Ho, G-T; Aird, R E; Liu, B et al. (2018) MDR1 deficiency impairs mitochondrial homeostasis and promotes intestinal inflammation. Mucosal Immunol 11:120-130
Pearce, Oliver M T; Delaine-Smith, Robin M; Maniati, Eleni et al. (2018) Deconstruction of a Metastatic Tumor Microenvironment Reveals a Common Matrix Response in Human Cancers. Cancer Discov 8:304-319

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