The Proteomics Core enhances the research productivity of KCI members by providing the equipment and trained personnel necessary for analysis of cellular proteome composition, protein modification, protein quantitation and protein interaction. The Proteomics Core is grouped in the Basic Research Core Cluster which, in addition to the Proteomics Core, includes the Microscopy, Imaging and Cytometry Resources Core and the Animal Model and Therapeutics Evaluation Core. The services provided by the Proteomics Core have contributed to 16 peer-reviewed publications during the current review period. Proteome profiling and protein identification services utilize state-of-the-art mass spectrometer-based methods. The platforms for analyses are the Thermo Fisher Orbitrap Fusion equipped with Electron Transfer Dissociation and the Thermo Fisher Q Exactive Orbitrap. Isolated protein, gel plug and full proteome analysis are supported. Sample preparation is achieved by robotic or manual depletion of high abundance proteins, chemical labeling, digestion and solid phase extraction (SPE). A full range of sorbents including specialized sorbents such as TiO2 for isolation of phosphopeptides are available for SPE. Nanoflow HPLC from Easy-nLC 1000 and Michrom H4 platforms is utilized for analyses with a Triversa Nanomate robot available as needed. Data analysis is achieved using Mascot, Sequest-HT, X!Tandem, MaxQuant and PEAKS algorithms with secondary data analysis by Scaffold Q+ and Scaffold PTM. Results are distributed electronically using our ftp server. The Core enhances research productivity by providing a clear and easily accessible process for protein identification and for relative quantitation of proteins based on isotopic labels. Quantitation technologies supported include Spectral Counting, cICAT, iTraq, TMT, SILAC and Multiple Reaction Monitoring (MRM). Analysis of isotopically labeled samples is achieved using Proteome Discoverer, MaxQuant and the Mascot Quantitation package as appropriate. MRM analysis is achieved using the TSQ Vantage with Skyline software for experimental design and data analysis. The protein identification and quantitation component of the Proteomics Core provides KCI members access to technology for protein identification, proteomic profiling and biomarker identification. The protein interactions component of the Core provides instrumentation and services for detection of protein binding by Fluorescence Polarization (FP). The instruments in the Core produce sensitive, accurate and real time measurements of protein binding events. Thus, the protein interactions component of the Core supports investigators in interrogating protein-protein interactions and the effects of those interactions on signaling pathways and cellular function.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA022453-34
Application #
8997274
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2016-02-01
Budget End
2016-11-30
Support Year
34
Fiscal Year
2016
Total Cost
$26,393
Indirect Cost
$9,180
Name
Wayne State University
Department
Type
DUNS #
001962224
City
Detroit
State
MI
Country
United States
Zip Code
48202
Su, Yongwei; Li, Xinyu; Ma, Jun et al. (2018) Targeting PI3K, mTOR, ERK, and Bcl-2 signaling network shows superior antileukemic activity against AML ex vivo. Biochem Pharmacol 148:13-26
Bonomi, Robin; Popov, Vadim; Laws, Maxwell T et al. (2018) Molecular Imaging of Sirtuin1 Expression-Activity in Rat Brain Using Positron-Emission Tomography-Magnetic-Resonance Imaging with [18F]-2-Fluorobenzoylaminohexanoicanilide. J Med Chem 61:7116-7130
Paximadis, Peter; Beebe-Dimmer, Jennifer L; George, Julie et al. (2018) Comparing Treatment Strategies for Stage I Small-cell lung Cancer. Clin Lung Cancer 19:e559-e565
Modi, Dipenkumar; Al-Kadhimi, Zaid; Chen, Wei et al. (2018) A phase II study of tacrolimus and thymoglobulin as graft-versus-host-disease prophylaxis in related donor allogeneic hematopoietic cell transplantation. Am J Hematol 93:E96-E98
Patki, Mugdha; McFall, Thomas; Rosati, Rayna et al. (2018) Chronic p27Kip1 Induction by Dexamethasone Causes Senescence Phenotype and Permanent Cell Cycle Blockade in Lung Adenocarcinoma Cells Over-expressing Glucocorticoid Receptor. Sci Rep 8:16006
Teslow, Emily A; Bao, Bin; Dyson, Greg et al. (2018) Exogenous IL-6 induces mRNA splice variant MBD2_v2 to promote stemness in TP53 wild-type, African American PCa cells. Mol Oncol 12:1138-1152
Rathinam, Rajamani; Rosati, Rita; Jamesdaniel, Samson (2018) CRISPR/Cas9-mediated knockout of Lim-domain only four retards organ of Corti cell growth. J Cell Biochem 119:3545-3553
Munkanatta Godage, Dhanushka N P; VanHecke, Garrett C; Samarasinghe, Kusal T G et al. (2018) SMYD2 glutathionylation contributes to degradation of sarcomeric proteins. Nat Commun 9:4341
Han, Jing; Li, Yue; Liu, Xiuli et al. (2018) Metformin suppresses retinal angiogenesis and inflammation in vitro and in vivo. PLoS One 13:e0193031
McKnight, Brooke N; Kuda-Wedagedara, Akhila N W; Sevak, Kuntal K et al. (2018) Imaging EGFR and HER3 through 89Zr-labeled MEHD7945A (Duligotuzumab). Sci Rep 8:9043

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