The Hybridoma Library/Monoclonal Antibody Production Shared Resource (Hybridoma Shared Resource) provides NCCC investigators with quality-controlled monoclonal antibodies (mAbs) as purified proteins an d/or supernatants from hybridoma cell lines that have been obtained from the ATCC or from individual investigators. This facility maintains characterized and quality-controlled stocks of supematants and monoclonal antibodies from more than one hundred cell lines. It is especially cost- effective for studies that require only a small sample of a given antibody and would otherwise result in a charge to individual grants of $250 to $500 per antibody. This Resource also advises investigators on both the creation of new hybridomas and the production and purification of large quantities of antibodies as needed. During the past year this Resource was used exclusively by NCCC investigators; there is no charge-back system for supply of these antibodies, which often come from patented cell lines. Over the past year, this Shared Resource is estimated to have achieved a net savings of between $41,000 and $100,000 over the commercial cost of comparable antibodies.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA023108-21
Application #
6101991
Study Section
Project Start
1999-04-01
Project End
1999-11-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
21
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Dartmouth College
Department
Type
DUNS #
041027822
City
Hanover
State
NH
Country
United States
Zip Code
03755
Jackson, Brian P (2018) Low level determination of gallium isotopes by ICP-QQQ. J Anal At Spectrom 33:897-900
Rahme, Gilbert J; Luikart, Bryan W; Cheng, Chao et al. (2018) A recombinant lentiviral PDGF-driven mouse model of proneural glioblastoma. Neuro Oncol 20:332-342
Barr, Fiona D; Ochsenbauer, Christina; Wira, Charles R et al. (2018) Neutrophil extracellular traps prevent HIV infection in the female genital tract. Mucosal Immunol 11:1420-1428
Sergent, P A; Plummer, S F; Pettus, J et al. (2018) Blocking the VISTA pathway enhances disease progression in (NZB?×?NZW) F1 female mice. Lupus 27:210-216
Kachuri, Linda; Saarela, Olli; Bojesen, Stig Egil et al. (2018) Mendelian Randomization and mediation analysis of leukocyte telomere length and risk of lung and head and neck cancers. Int J Epidemiol :
Wang, Zhaoxi; Wei, Yongyue; Zhang, Ruyang et al. (2018) Multi-Omics Analysis Reveals a HIF Network and Hub Gene EPAS1 Associated with Lung Adenocarcinoma. EBioMedicine 32:93-101
Shee, Kevin; Jiang, Amanda; Varn, Frederick S et al. (2018) Cytokine sensitivity screening highlights BMP4 pathway signaling as a therapeutic opportunity in ER+ breast cancer. FASEB J :fj201801241R
Rodriguez-Garcia, Marta; Fortier, Jared M; Barr, Fiona D et al. (2018) Aging impacts CD103+ CD8+ T cell presence and induction by dendritic cells in the genital tract. Aging Cell 17:e12733
Shajani-Yi, Zahra; de Abreu, Francine B; Peterson, Jason D et al. (2018) Frequency of Somatic TP53 Mutations in Combination with Known Pathogenic Mutations in Colon Adenocarcinoma, Non-Small Cell Lung Carcinoma, and Gliomas as Identified by Next-Generation Sequencing. Neoplasia 20:256-262
Szczepiorkowski, Zbigniew M; Burnett, Christine A; Dumont, Larry J et al. (2018) Apheresis buffy coat collection without photoactivation has no effect on apoptosis, cell proliferation, and total viability of mononuclear cells collected using photopheresis systems. Transfusion 58:943-950

Showing the most recent 10 out of 1911 publications