Genomics and Molecular Biology The Genomics and Molecular Biology (GMB) Shared Resource provides advanced genomics technologies, services, and expertise in support of biomedical research conducted at the Norris Cotton Cancer Center (NCCC) and Dartmouth College. GMB operates as a fee-for-service facility offering sequencing of DNA and RNA, microarray technologies, single-cell DNA/RNA sequencing, NanoString assays, and next-day Sanger sequencing services. GMB also provides expertise for assay design and primary data analysis as complementary services. Improvements to GMB in the past several years include a new and improved sample tracking and billing system (RaDar), as well as up-grades in instrumentation and protocols used. Additional improvements include added expertise of new personnel and services for single-cell sequencing, new Nanostring assays, and mouse SNP genotyping. GMB and its committed staff have a combined 130+ years of clinical- and research-related laboratory experience. This expertise and experience facilitate GMB?s ability to operate state-of-the-art instrumentation and implement cutting-edge workflows, providing NCCC researchers with access to essential genomics technologies in this fast-moving field. It is also essential for GMB to stay competitive with market rates while providing additional value to its users over external service providers. Strong institutional support and GMB leadership ensure its stability. GMB leadership works diligently to recruit new projects to the shared resource through participation in both internal and external seminars and conferences. Personnel within GMB stay abreast of new technology developments through conference attendance as well as membership in the Association of Biomolecular Research Facilities (ABRF). GMB serves Cancer Center members, the broader Dartmouth research community, and users from outside institutions, operating at ~80% capacity. Sixty-one (61) NCCC Members from all 4 NCCC Programs utilized the GMB Shared Resource, 60 of whom are current Members in 4 Programs (CPS [6], CBT [37], ICI [14], and TEC [3]). NCCC Funded Member Use represented 31% of Total Use (4,779 Projects), and we are requesting 19% of the GMB Total Operating Budget from CCSG support. Priority is given to NCCC member samples, followed by Dartmouth samples, and then outside samples. GMB maintains stringent quality control procedures over samples entering and data leaving the facility. Appropriate controls are run regularly on all instrumentation, and run metrics are tracked over time to ensure optimal performance. Future plans involve implementing new single-cell workflows highly sought by NCCC researchers, protein and antigen barcoding, and DNA and ATAC- seq workflows. Talks are underway to acquire spatial genomic technologies, such as the Nanostring Digital Spatial Profiler, as a complement to droplet-based single-cell profiling. GMB is implementing solutions for genome-wide detection of CRISPR/Cas-9 editing, including the GUIDE-seq and CIRCLE-seq methodologies. GMB is committed to providing NCCC researchers with the latest genomics expertise and technologies.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA023108-41
Application #
9855306
Study Section
Subcommittee I - Transistion to Independence (NCI)
Project Start
Project End
Budget Start
2019-12-01
Budget End
2020-11-30
Support Year
41
Fiscal Year
2020
Total Cost
Indirect Cost
Name
Dartmouth College
Department
Type
DUNS #
041027822
City
Hanover
State
NH
Country
United States
Zip Code
03755
Gacerez, Albert T; Hua, Casey K; Ackerman, Margaret E et al. (2018) Chimeric antigen receptors with human scFvs preferentially induce T cell anti-tumor activity against tumors with high B7H6 expression. Cancer Immunol Immunother 67:749-759
Onega, T; Zhu, W; Weiss, J E et al. (2018) Preoperative breast MRI and mortality in older women with breast cancer. Breast Cancer Res Treat 170:149-157
Vergo, Maxwell T; Pinkson, Briane M; Broglio, Kathleen et al. (2018) Immediate Symptom Relief After a First Session of Massage Therapy or Reiki in Hospitalized Patients: A 5-Year Clinical Experience from a Rural Academic Medical Center. J Altern Complement Med 24:801-808
Varn, Frederick S; Schaafsma, Evelien; Wang, Yue et al. (2018) Genomic Characterization of Six Virus-Associated Cancers Identifies Changes in the Tumor Immune Microenvironment and Altered Genetic Programs. Cancer Res 78:6413-6423
Hill, Deirdre A; Haas, Jennifer S; Wellman, Robert et al. (2018) Utilization of breast cancer screening with magnetic resonance imaging in community practice. J Gen Intern Med 33:275-283
Lott, Jason P; Boudreau, Denise M; Barnhill, Ray L et al. (2018) Population-Based Analysis of Histologically Confirmed Melanocytic Proliferations Using Natural Language Processing. JAMA Dermatol 154:24-29
Jackson, Brian P (2018) Low level determination of gallium isotopes by ICP-QQQ. J Anal At Spectrom 33:897-900
Rahme, Gilbert J; Luikart, Bryan W; Cheng, Chao et al. (2018) A recombinant lentiviral PDGF-driven mouse model of proneural glioblastoma. Neuro Oncol 20:332-342
Barr, Fiona D; Ochsenbauer, Christina; Wira, Charles R et al. (2018) Neutrophil extracellular traps prevent HIV infection in the female genital tract. Mucosal Immunol 11:1420-1428
Sergent, P A; Plummer, S F; Pettus, J et al. (2018) Blocking the VISTA pathway enhances disease progression in (NZB?×?NZW) F1 female mice. Lupus 27:210-216

Showing the most recent 10 out of 1911 publications