Life Sciences Mass Spectrometry Facility Shared Resource (LSMSF-SR): Project Summary Mass spectrometry plays key roles across a wide range of atomic and molecular analyses relevant to the life sciences in general and cancer research specifically. Mass spectrometry, as a category within measurement science, is comprised of a variety of technologies and methodologies that provide qualitative and quantitative information based on the measurement of atomic and molecular mass. Measurements made by Purdue Center for Cancer Research (PCCR) members range from analytes as diverse as atomic species, to large multi-unit bio-complexes, and involve a variety of compound classes that include, inter alia, small molecule drugs, lipids, nucleic acids, and proteins. Mass spectrometry has experienced a dramatic expansion in its range of applications over the past several decades due to major technological developments that have led to new and improved approaches to making ions, measuring ions, and probing ions. Examples of revolutionary ionization methods, for example, include electrospray ionization, which has been key to the major expansion of liquid chromatography coupled with mass spectrometry, and matrix-assisted laser desorption-ionization, which has been key to advances in ion imaging. Major developments in instrumentation have included advances in time-of-flight mass spectrometry and Fourier transform mass spectrometry approaches, particularly with the electrostatic ion trap referred to as the OrbitrapTM. Novel ion probes have been introduced, such as electron transfer dissociation and ion mobility. Collectively, these developments have enabled a veritable explosion of mass spectrometry applications in the life sciences. As a result, the types of measurements that cancer researchers seek to make with mass spectrometry requires a growing range of tools and expertise. To address this challenge, in 2018 Purdue coordinated all of the life- sciences-oriented mass spectrometry services within the Life Sciences Mass Spectrometry Facility, a shared resource of the Purdue Center for Cancer Research. This Facility is comprised of 11 staff and roughly two dozen mass spectrometers that support a wide range of measurements relevant to the needs of PCCR members that address both qualitative and quantitative questions. The tools and expertise of this Shared Resource are devoted to providing for today's research needs of PCCR members as well as adapting to and anticipating future needs.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA023168-40
Application #
10024915
Study Section
Subcommittee I - Transistion to Independence (NCI)
Project Start
Project End
Budget Start
2020-08-01
Budget End
2021-06-30
Support Year
40
Fiscal Year
2020
Total Cost
Indirect Cost
Name
Purdue University
Department
Type
DUNS #
072051394
City
West Lafayette
State
IN
Country
United States
Zip Code
47907
Huang, Xinxin; Guo, Bin; Liu, Sheng et al. (2018) Neutralizing negative epigenetic regulation by HDAC5 enhances human haematopoietic stem cell homing and engraftment. Nat Commun 9:2741
Chambers, Andrea M; Lupo, Kyle B; Matosevic, Sandro (2018) Tumor Microenvironment-Induced Immunometabolic Reprogramming of Natural Killer Cells. Front Immunol 9:2517
Shinde, Aparna; Wilmanski, Tomasz; Chen, Hao et al. (2018) Pyruvate carboxylase supports the pulmonary tropism of metastatic breast cancer. Breast Cancer Res 20:76
Nenortas, Nathaniel P; Cinelli, Maris A; Morrell, Andrew E et al. (2018) Activity of Aromathecins against African Trypanosomes. Antimicrob Agents Chemother 62:
Norvil, Allison B; Petell, Christopher J; Alabdi, Lama et al. (2018) Dnmt3b Methylates DNA by a Noncooperative Mechanism, and Its Activity Is Unaffected by Manipulations at the Predicted Dimer Interface. Biochemistry 57:4312-4324
Chambers, Andrea M; Wang, Jiao; Lupo, Kyle B et al. (2018) Adenosinergic Signaling Alters Natural Killer Cell Functional Responses. Front Immunol 9:2533
Serratore, Nina D; Baker, Kortany M; Macadlo, Lauren A et al. (2018) A Novel Sterol-Signaling Pathway Governs Azole Antifungal Drug Resistance and Hypoxic Gene Repression in Saccharomyces cerevisiae. Genetics 208:1037-1055
Wu, Heng; Post, Carol Beth (2018) Protein Conformational Transitions from All-Atom Adaptively Biased Path Optimization. J Chem Theory Comput 14:5372-5382
Denton, Kyle E; Wang, Sijie; Gignac, Michael C et al. (2018) Robustness of In Vitro Selection Assays of DNA-Encoded Peptidomimetic Ligands to CBX7 and CBX8. SLAS Discov 23:417-428
Liu, Wenting; Zhong, Yi-Fang; Liu, Liu-Yi et al. (2018) Solution structures of multiple G-quadruplex complexes induced by a platinum(II)-based tripod reveal dynamic binding. Nat Commun 9:3496

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