? ANIMAL RESOURCES SHARED RESOURCE The Animal Resources Shared Resource is a Cancer Center-managed Shared Resource that provides a comprehensive animal care and use program for Center investigators. The facility has been AAALAC-accredited since 1988. The Core provides five basic types of services: (1) husbandry and equipment sanitation/sterilization, (2) mouse breeding procedures and maintenance; (3) procedural techniques training and support; (4) in vivo imaging, blood analysis, and irradiation; and (5) veterinary services and administrative/IACUC support. The animal facility currently occupies 25,602 sq. ft of space in four buildings. Mice are the primary species used, with occasional housing of rats. Over the last 5-year grant period, AR averaged 8,389 occupied mouse cages per month, with growth in the last 2 years to more than 9,000 cages/month. The mice are housed in individually ventilated caging systems. There is sufficient capacity for growth, as the facility is capable of maintaining up to 13,000 cages but generally operates at ~70% capacity, which facilitates breeding colony maintenance and protocol housing requirements. AR provides full-service animal care that includes all husbandry as well as setting up breedings, weaning litters, obtaining tail samples, maintaining animal pedigrees, census and IDs. This comprehensive service is also cost-effective, with the per diem including those services at $0.72/cage for standard mice. The AR staff perform an additional range of services for Center investigators, including intravenous (tail and retro-orbital veins), subcutaneous, intradermal, intramuscular, intraperitoneal, and mammary fat pad injections, oral gavage, tumor measurements, blood and tissue collection, body weights, perfusions, and pre- and post-operative surgical care. The Core provides essential scientific support to Center members by supplying animal models and expert technical assistance for investigation of tumor development and growth in vivo. The Tumor Analysis facility is a key resource in this respect, providing access to a variety of xenograft models, including patient-derived xenografts, and further assisting with the experimental design and carrying out studies of genetic factors or experimental compounds that influence tumor growth and metastasis. This service is supported by an array of sophisticated imaging modalities for evaluating tumor progression, including luminescence, fluorescence, and ultrasound. Additional Animal Analysis services include blood cell, serum, behavioral, and metabolic analysis. In the past five years, 44 Investigators from all three Cancer Center programs have made extensive use of the Animal Resources, and the Core has provided support for the publication of 116 cancer-relevant papers by Cancer Center investigators.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA030199-39
Application #
9934918
Study Section
Special Emphasis Panel (ZCA1)
Project Start
Project End
Budget Start
2020-05-27
Budget End
2021-04-30
Support Year
39
Fiscal Year
2020
Total Cost
Indirect Cost
Name
Sanford Burnham Prebys Medical Discovery Institute
Department
Type
DUNS #
020520466
City
La Jolla
State
CA
Country
United States
Zip Code
92037
Ekanayake, Vindana; Nisan, Danielle; Ryzhov, Pavel et al. (2018) Lipoprotein Particle Formation by Proapoptotic tBid. Biophys J 115:533-542
Diez-Cuñado, Marta; Wei, Ke; Bushway, Paul J et al. (2018) miRNAs that Induce Human Cardiomyocyte Proliferation Converge on the Hippo Pathway. Cell Rep 23:2168-2174
Wang, Yang; Li, Yue; Yue, Minghui et al. (2018) N6-methyladenosine RNA modification regulates embryonic neural stem cell self-renewal through histone modifications. Nat Neurosci 21:195-206
Lundquist, Mark R; Goncalves, Marcus D; Loughran, Ryan M et al. (2018) Phosphatidylinositol-5-Phosphate 4-Kinases Regulate Cellular Lipid Metabolism By Facilitating Autophagy. Mol Cell 70:531-544.e9
Ramirez, Monica L Gonzalez; Poreba, Marcin; Snipas, Scott J et al. (2018) Extensive peptide and natural protein substrate screens reveal that mouse caspase-11 has much narrower substrate specificity than caspase-1. J Biol Chem 293:7058-7067
Wei, Yang; Toth, Julia I; Blanco, Gabrielle A et al. (2018) Adapted ATPase domain communication overcomes the cytotoxicity of p97 inhibitors. J Biol Chem 293:20169-20180
Tinoco, Roberto; Carrette, Florent; Henriquez, Monique L et al. (2018) Fucosyltransferase Induction during Influenza Virus Infection Is Required for the Generation of Functional Memory CD4+ T Cells. J Immunol 200:2690-2702
Wonder, Emily; Simón-Gracia, Lorena; Scodeller, Pablo et al. (2018) Competition of charge-mediated and specific binding by peptide-tagged cationic liposome-DNA nanoparticles in vitro and in vivo. Biomaterials 166:52-63
Limpert, Allison S; Lambert, Lester J; Bakas, Nicole A et al. (2018) Autophagy in Cancer: Regulation by Small Molecules. Trends Pharmacol Sci 39:1021-1032
Fujita, Yu; Khateb, Ali; Li, Yan et al. (2018) Regulation of S100A8 Stability by RNF5 in Intestinal Epithelial Cells Determines Intestinal Inflammation and Severity of Colitis. Cell Rep 24:3296-3311.e6

Showing the most recent 10 out of 599 publications