? CANCER METABOLISM SHARED RESOURCE The Cancer Metabolism Shared Resource provides comprehensive support for analysis of cancer-related metabolism by investigators at the Sanford Burnham Prebys Medical Discovery Institute NCI-supported Cancer Center. The services provided include assistance with the design of metabolic research programs and selection of appropriate analytical methods, sample analysis using specialized equipment and methods, assistance with data interpretation and publication (including response to reviewers), and assistance with grant proposals. Dr. Scott, the facility Director, has extensive experience in metabolic analysis and serves as a resource for Center members, educating scientists about the services offered, working to upgrade existing methods, and expanding the range of analyses provided, both in response to requests from users and proactively in anticipation of future demand. The Core provides three fundamental types of analysis. The first is quantitation of metabolites, either through GC-MS analysis or a 96-well YSI analyzer. In the past funding period (the first for this Shared Resource), a variety of enhancements have been made in quantification of metabolites such as fatty acids (including short- chain fatty acids), cholesterol, sugars, and sugar phosphates, as well as more standard detection of glucose, lactate, glutamine, and glutamate. The second approach provided in the Core is stable isotope tracing, where(13C, 15N, 2H)-labeled substrates can be traced to metabolites such as TCA cycle intermediates and fatty acids, which provides information on metabolic pathway flux that provides insight beyond metabolite quantification alone. The third major analytical platform in the Core is a Seahorse XFp Extracellular Flux Analyzer, purchased in 2015, which allows real-time measurement of cellular oxygen consumption and extracellular acidification. Mitochondrial function and the relative contribution of glycolysis and oxidative phosphorylation to ATP production can be measured in live by sequential addition of various mitochondrial inhibitors or uncouplers. Over the last 5 years, the Cancer Metabolism Core has provided services to 24 Cancer Center members, and supported at least 20 publications

National Institute of Health (NIH)
National Cancer Institute (NCI)
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Sanford Burnham Prebys Medical Discovery Institute
La Jolla
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Ekanayake, Vindana; Nisan, Danielle; Ryzhov, Pavel et al. (2018) Lipoprotein Particle Formation by Proapoptotic tBid. Biophys J 115:533-542
Diez-Cuñado, Marta; Wei, Ke; Bushway, Paul J et al. (2018) miRNAs that Induce Human Cardiomyocyte Proliferation Converge on the Hippo Pathway. Cell Rep 23:2168-2174
Wang, Yang; Li, Yue; Yue, Minghui et al. (2018) N6-methyladenosine RNA modification regulates embryonic neural stem cell self-renewal through histone modifications. Nat Neurosci 21:195-206
Lundquist, Mark R; Goncalves, Marcus D; Loughran, Ryan M et al. (2018) Phosphatidylinositol-5-Phosphate 4-Kinases Regulate Cellular Lipid Metabolism By Facilitating Autophagy. Mol Cell 70:531-544.e9
Ramirez, Monica L Gonzalez; Poreba, Marcin; Snipas, Scott J et al. (2018) Extensive peptide and natural protein substrate screens reveal that mouse caspase-11 has much narrower substrate specificity than caspase-1. J Biol Chem 293:7058-7067
Wei, Yang; Toth, Julia I; Blanco, Gabrielle A et al. (2018) Adapted ATPase domain communication overcomes the cytotoxicity of p97 inhibitors. J Biol Chem 293:20169-20180
Tinoco, Roberto; Carrette, Florent; Henriquez, Monique L et al. (2018) Fucosyltransferase Induction during Influenza Virus Infection Is Required for the Generation of Functional Memory CD4+ T Cells. J Immunol 200:2690-2702
Wonder, Emily; Simón-Gracia, Lorena; Scodeller, Pablo et al. (2018) Competition of charge-mediated and specific binding by peptide-tagged cationic liposome-DNA nanoparticles in vitro and in vivo. Biomaterials 166:52-63
Limpert, Allison S; Lambert, Lester J; Bakas, Nicole A et al. (2018) Autophagy in Cancer: Regulation by Small Molecules. Trends Pharmacol Sci 39:1021-1032
Fujita, Yu; Khateb, Ali; Li, Yan et al. (2018) Regulation of S100A8 Stability by RNF5 in Intestinal Epithelial Cells Determines Intestinal Inflammation and Severity of Colitis. Cell Rep 24:3296-3311.e6

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