PROVIDED.Mass spectrometry has become the principal method to identify and characterize proteins and othermacromolecular structures that are the functional machinery for biological processes. The purpose of theMass Spectrometry & Proteomics Facility (MSPF) is to provide Cancer Center members with essentialservices in macromolecular structural analysis. These services include electrospray and MALDI massspectrometry for molecular mass determination, tandem mass spectrometry (MSn) for detailed structureanalysis (i.e., peptide sequencing), LC/MS/MS analysis for analyzing complex biological mixtures, anddatabase searching for protein identification. The facility has two electrospray ion trap (LC/MS) systems(ThermoElectron LCQ and LTQ) and a high performance MALDI qToF system (PE/Sciex prOTof). Thefacility is operated both on a fee-for-service and shared use (researchers run their own samples) basis.Facility personnel provide expertise on sample preparation, analysis and data interpretation. They alsoassist experimental design and the development of novel methodologies. During the most recent 12-monthreporting period, the CCMSPF was used by 19 Cancer Center members from all five Research Programs.Peer-reviewed usage represented 72% of total usage. Annual budget for this core is $408,887, of which69% is institutional funding, 11% is user fees, and 20% ($81,000) is requested from the CCSG.
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