Abstract

) The Histology Shared Resource provides the following basic histology services: (I) tissue processing; (ii) embedding of tissues and cells; and (iii) cutting and staining of tissues. Several staining procedures, including H&E, histochemistry, and immunohistochemistry can be used. In addition, tissues are processed for autoradiography and immunoelectron microscopy. Immunohistochemical staining procedures can be performed to demonstrate up to four different antigens in the same tissue and generating slides that are permanent. In addition, immunoelectron microscopic procedures capable of localizing up to three antigens simultaneously at the subcellular level using gold particles of different sizes is available. In- situ hybridization procedures for the demonstration of mRNA of various factors with available primers are also performed in the Histology Shared Resource.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA036727-16
Application #
6345989
Study Section
Project Start
2000-09-01
Project End
2001-07-31
Budget Start
Budget End
Support Year
16
Fiscal Year
2000
Total Cost
Indirect Cost

  Institution

Name
University of Nebraska Medical Center
Department
Type
DUNS #
City
Omaha
State
NE
Country
United States
Zip Code
68198

  Publications

Barkeer, Srikanth; Chugh, Seema; Karmakar, Saswati et al. (2018) Novel role of O-glycosyltransferases GALNT3 and B3GNT3 in the self-renewal of pancreatic cancer stem cells. BMC Cancer 18:1157
Cserhati, Matyas F; Mooter, Mary-Ellen; Peterson, Lauren et al. (2018) Motifome comparison between modern human, Neanderthal and Denisovan. BMC Genomics 19:472
Napon, Geoffroy; Dafferner, Alicia J; Saxena, Ashima et al. (2018) Identification of Carboxylesterase, Butyrylcholinesterase, Acetylcholinesterase, Paraoxonase, and Albumin Pseudoesterase in Guinea Pig Plasma through Nondenaturing Gel Electrophoresis. Comp Med 68:367-374
Luan, Haitao; Mohapatra, Bhopal; Bielecki, Timothy A et al. (2018) Loss of the Nuclear Pool of Ubiquitin Ligase CHIP/STUB1 in Breast Cancer Unleashes the MZF1-Cathepsin Pro-oncogenic Program. Cancer Res 78:2524-2535
Rana, Sandeep; Sonawane, Yogesh A; Taylor, Margaret A et al. (2018) Synthesis of aminopyrazole analogs and their evaluation as CDK inhibitors for cancer therapy. Bioorg Med Chem Lett 28:3736-3740
Azadmanesh, Jahaun; Borgstahl, Gloria E O (2018) A Review of the Catalytic Mechanism of Human Manganese Superoxide Dismutase. Antioxidants (Basel) 7:
Chhonker, Yashpal S; Haney, Staci L; Matthiesen, Robert A et al. (2018) Quantitative determination of a potent geranylgeranyl diphosphate synthase inhibitor using LC-MS/MS: Derivatization and application. J Pharm Biomed Anal 153:22-28
Haney, Staci L; Chhonker, Yashpal S; Varney, Michelle L et al. (2018) Preclinical investigation of a potent geranylgeranyl diphosphate synthase inhibitor. Invest New Drugs 36:810-818
Saxena, Sugandha; Hayashi, Yuri; Wu, Lingyun et al. (2018) Pathological and functional significance of Semaphorin-5A in pancreatic cancer progression and metastasis. Oncotarget 9:5931-5943
Lockridge, Oksana; David, Emilie; Schopfer, Lawrence M et al. (2018) Purification of recombinant human butyrylcholinesterase on Hupresin®. J Chromatogr B Analyt Technol Biomed Life Sci 1102-1103:109-115

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