The Proteomics Shared Resource (PSR) is a specialized technical facility with the primary goal of protein identification, characterization and quantitation, and is open to all Cancer Center members at CSHL. The Proteomics Shared Resource is located within the W.M. Keck Structural Biology Laboratory on the ground floor ofthe Beckman building. In the last 18 months CSHL has completed a major restructuring ofthe Resource. A senior faculty member with significant expertise in proteomics was recruited to redesign and oversee the Shared Resource. In addition, a PhD-level scientist with experience in proteomics and mass spectrometry was recruited to manage and run the facility. With the new staff in place, state-of-the-art equipment was purchased and the laboratory space was extensively renovated. The Resource features new, state-of-the-art equipment, including: one Thermo LTQ-Orbitrap mass spectrometer, one Thermo LTQ iontrap mass spectrometer, one Agilent 6520 quadrupole time-of-flight (QTOF) mass spectrometer and one Thermo Vantage triple quadrupole mass spectrometer, all fitted with nano electrospray (NanoESl) sources. A second Orbitrap Velos mass spectrometer with ETD source (funded through the High-End Instrumentation program) is due to be installed in late 2010. An additional Accela HPLC and autosampler is also available for analytical scale MRM work using the Vantage triple-Quad.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA045508-24
Application #
8340295
Study Section
Subcommittee G - Education (NCI)
Project Start
2011-08-17
Project End
2016-07-31
Budget Start
2011-08-17
Budget End
2012-07-31
Support Year
24
Fiscal Year
2011
Total Cost
$346,186
Indirect Cost
Name
Cold Spring Harbor Laboratory
Department
Type
DUNS #
065968786
City
Cold Spring Harbor
State
NY
Country
United States
Zip Code
11724
Nattestad, Maria; Goodwin, Sara; Ng, Karen et al. (2018) Complex rearrangements and oncogene amplifications revealed by long-read DNA and RNA sequencing of a breast cancer cell line. Genome Res 28:1126-1135
Connell, Claire M; Raby, Sophie E M; Beh, Ian et al. (2018) Cancer Immunotherapy Trials Underutilize Immune Response Monitoring. Oncologist 23:116-117
Wong, Mandy S; Kinney, Justin B; Krainer, Adrian R (2018) Quantitative Activity Profile and Context Dependence of All Human 5' Splice Sites. Mol Cell 71:1012-1026.e3
Snider, Justin M; Snider, Ashley J; Obeid, Lina M et al. (2018) Probing de novo sphingolipid metabolism in mammalian cells utilizing mass spectrometry. J Lipid Res 59:1046-1057
Stacchiotti, Silvia; Mir, Olivier; Le Cesne, Axel et al. (2018) Activity of Pazopanib and Trabectedin in Advanced Alveolar Soft Part Sarcoma. Oncologist 23:62-70
Banito, Ana; Li, Xiang; Laporte, Aimée N et al. (2018) The SS18-SSX Oncoprotein Hijacks KDM2B-PRC1.1 to Drive Synovial Sarcoma. Cancer Cell 33:527-541.e8
Goncalves, Marcus D; Hwang, Seo-Kyoung; Pauli, Chantal et al. (2018) Fenofibrate prevents skeletal muscle loss in mice with lung cancer. Proc Natl Acad Sci U S A 115:E743-E752
Hwang, Dong-Woo; Jaganathan, Anbalagan; Shrestha, Padmina et al. (2018) Chromatin-mediated translational control is essential for neural cell fate specification. Life Sci Alliance 1:e201700016
Knott, Simon R V; Wagenblast, Elvin; Khan, Showkhin et al. (2018) Asparagine bioavailability governs metastasis in a model of breast cancer. Nature 554:378-381
Biffi, Giulia; Oni, Tobiloba E; Spielman, Benjamin et al. (2018) IL-1-induced JAK/STAT signaling is antagonized by TGF-beta to shape CAF heterogeneity in pancreatic ductal adenocarcinoma. Cancer Discov :

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