The mission of the Genomics Shared Resource (GSR) is to provide state-of-the-art genomic services to Cancer Therapy and Research Center (CTRC) members in an economical and timely manner. The GSR has been in operation since the inception of the Cancer Center Support Grant (CCSG) in the early 1990's. Originally, the shared resource focused on cytogenetic services under the leadership of Dr. John McGill. Dr. McGill left the University in 2000, and Dr. Robin Leach became Director of the Cytogenetics Shared Resource. Although the Shared Resource began expanding to non-cytogenetic services in 2001, it was only renamed the Genomics Shared Resource in the 2008 competitive renewal of the CCSG. The GSR is located within 1,470 sq.ft. on the UTHSCSA Long Campus. The GSR provides state-of-the-art services as well as intellectual expertise with a range of molecular genetic assays that complement the interests, needs and existing resources of its users. The GSR provides access to both high throughput and custom genotyping of single nucleotide polymorphisms using the lllumina iScan and BeadXpressGoldenGate technologies and TaqMan allelic discrimination analysis on the Life Technologies 7900HT Sequence Detection System. It also offers whole genome gene expression microarray analysis using the lllumina iScan. In addition, the GSR offers services for quantity and quality assessment of nucleic acids (Nanodrop spectrophotometer) and Agilent Bioanalyzer. Finally, the GSR provides support for sample processing by providing services for isolating nucleic acids, as well as transforming lymphocytes to establish lymphoblastoid cell lines using Epstein-Barr virus. The GSR staff consists of the Director, Robin J. Leach, Ph.D., Co-Director, Teresa L. Johnson-Pais, Ph.D. and Research Associate, Mandy R. Hinojosa. GSR services supported 10- peer-reviewed funded cancer center members, which accounted for 29% of the total users and 47% of the samples evaluated by the core during the last award year.
In this era of personalized medicine, genetic variation has been shown to be important in both the development and progression of cancer and genetic variants have also been utilized to predict response to clinical therapies. In addition, gene expression studies have proven useful for stratifying cancers and understanding the role of specific genes. The GSR provides the expertise and newest technologies for both genotyping and gene expression studies to cancer center members.
|Sun, Xiujie; Gupta, Kshama; Wu, Bogang et al. (2018) Tumor-extrinsic discoidin domain receptor 1 promotes mammary tumor growth by regulating adipose stromal interleukin 6 production in mice. J Biol Chem 293:2841-2849|
|Horning, Aaron M; Wang, Yao; Lin, Che-Kuang et al. (2018) Single-Cell RNA-seq Reveals a Subpopulation of Prostate Cancer Cells with Enhanced Cell-Cycle-Related Transcription and Attenuated Androgen Response. Cancer Res 78:853-864|
|Gong, Siqi; Tomusange, Khamis; Kulkarni, Viraj et al. (2018) Anti-HIV IgM protects against mucosal SHIV transmission. AIDS 32:F5-F13|
|Soteros, Breeanne M; Cong, Qifei; Palmer, Christian R et al. (2018) Sociability and synapse subtype-specific defects in mice lacking SRPX2, a language-associated gene. PLoS One 13:e0199399|
|Gelfond, Jonathan; Goros, Martin; Hernandez, Brian et al. (2018) A System for an Accountable Data Analysis Process in R. R J 10:6-21|
|De La Chapa, Jorge J; Singha, Prajjal Kanti; Lee, Debbie R et al. (2018) Thymol inhibits oral squamous cell carcinoma growth via mitochondria-mediated apoptosis. J Oral Pathol Med 47:674-682|
|Katti, Sachin; Her, Bin; Srivastava, Atul K et al. (2018) High affinity interactions of Pb2+ with synaptotagmin I. Metallomics 10:1211-1222|
|Hariharan, Nisha; Ashcraft, Keith A; Svatek, Robert S et al. (2018) Adipose Tissue-Secreted Factors Alter Bladder Cancer Cell Migration. J Obes 2018:9247864|
|Wang, Zhonghua; Bhattacharya, Akash; White, Tommy et al. (2018) Functionality of Redox-Active Cysteines Is Required for Restriction of Retroviral Replication by SAMHD1. Cell Rep 24:815-823|
|Deng, Yilun; Qin, Yuejuan; Srikantan, Subramanya et al. (2018) The TMEM127 human tumor suppressor is a component of the mTORC1 lysosomal nutrient-sensing complex. Hum Mol Genet 27:1794-1808|
Showing the most recent 10 out of 989 publications