FLOW CYTOMETRY SHARED RESOURCEThe Flow Cytometry Shared Resource provides state-of-the-art cell sorting and analysis capability to themembers of the Kimmel Cancer Center at Thomas Jefferson University. This shared resource is locatedin a dedicated laboratory space of about 600 sq ft on the 6th floor of the Bluemle Life Sciences Building,centrally positioned on the Thomas Jefferson University campus. The Flow Cytometry Shared Resourceis equiped with one Cytomation MoFlo cell high-speed sorter, one Coutler EPICS Elite flow cytometercapable of sorting, one Coulter XL-MCL automated analytical cytometer, one FACS Calibur BenchtopAnalyzer, one FACScan Benchtop Analyzer, two Celeron 400 workstations, one Nikon fluorescencemicroscope, a 30 cu ft 4 C refrigerator, and a 10 cu ft -20 C freezer. All units are maintained andmanaged by a dedicated manager/operator. The most commonly used applications are for surfacephenotyping and sorting, detection of intracellular cytokines at the single cell level in mixed populations,single cell real time analysis of Ca2* mobilization kinetics, studies of apoptosis, cell cycle analysis, andsorting of viable cells in specific stages of the cell cycle. This shared resource allows experimental anddata analysis to be performed in a timely manner and with high sensitivity and accuracy. Peer-review,funded cancer center investigators receive priority for the use of this shared resource. Based onincreased capabilities for new services, the steady increase in the use of this resource by cancer centermembers and the recruitment of new investigators, we project that shared resource usage will increasesignificantly in the near future. In summary, this shared resource plays a critical role in assisting cancercenter investigators as they conduct research into the biology and treatment of cancer.
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