Modern cancer research requires state-of-the-art cell and tissue imaging technology. These techniques include immunolocalization studies at both the light and electron microscopic levels, live cell studies using molecules tagged with fluorescent probes and introduced by transfection or microinjection, and advanced image recording and analysis. Sophisticated imaging instruments such as electron microscopes, confocal and multi-photon microscopes are not only very expensive, but also require technical assistance for optimal results. For these reasons, it is essential to have a core imaging facility that provides access to both the instruments and technical expertise. Established more than 20 years ago as a departmental service, the Cell Imaging Facility has evolved into a Medical School Core Facility that receives support from the medical school, the Vice President for Research at NU, and the Robert H. Lurie Comprehensive Cancer Center. The Facility's Director, Dr. Teng-Leong Chew, Ph.D., is an expert in light microscopic imaging, especially in advanced live cell fluorescence microscopy. A full time electron microscope technologist is also available to perform routine cell and tissue embedding and sectioning as well as advanced techniques such as immunogold localization, and to train users in these techniques. The facility serves several hundred users from more than 100 research groups throughout the University. Due to the unique expertise as well as the staffs commitment to developing cutting-edge imaging application, the facility has been selected by numerous leading manufacturers as the beta test sites for their instruments and software. More importantly, the facility has been recognized as one of the three National Imaging Centers of Excellence by Nikon, thus benefiting Northwestern immensely through Nikon's gift of several state-of the-art equipments. Approximately 50 percent of total facility usage has been by Cancer Center members with peer reviewed funding. Equipment includes two transmission electron microscopes, two confocal microscopes, one spinning disc confocal, total internal reflection fluorescence (TIRF) microscope, three advanced light microscopes with digital imaging systems and capabilities that include microinjection, epifluoresence, time lapse photography, calcium imaging, FRAP, and FRET. Ancillary equipment includes rotary shadowing evaporators, microtomes, color printers, digital scanner, film recorder, dark room, and computers and software for image analysis.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA060553-16
Application #
7920984
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2009-08-01
Budget End
2010-07-31
Support Year
16
Fiscal Year
2009
Total Cost
$181,751
Indirect Cost
Name
Northwestern University at Chicago
Department
Type
DUNS #
005436803
City
Chicago
State
IL
Country
United States
Zip Code
60611
Ritzert, Jeremy T; Lathem, Wyndham W (2018) Depletion of Glucose Activates Catabolite Repression during Pneumonic Plague. J Bacteriol 200:
Bui, Triet M; Mascarenhas, Lorraine A; Sumagin, Ronen (2018) Extracellular vesicles regulate immune responses and cellular function in intestinal inflammation and repair. Tissue Barriers 6:e1431038
Park, Yun Ji; Kenney, Grace E; Schachner, Luis F et al. (2018) Repurposed HisC Aminotransferases Complete the Biosynthesis of Some Methanobactins. Biochemistry 57:3515-3523
Forte, Eleonora; Swaminathan, Suchitra; Schroeder, Mark W et al. (2018) Tumor Necrosis Factor Alpha Induces Reactivation of Human Cytomegalovirus Independently of Myeloid Cell Differentiation following Posttranscriptional Establishment of Latency. MBio 9:
Mandelin 2nd, Arthur M; Homan, Philip J; Shaffer, Alexander M et al. (2018) Transcriptional Profiling of Synovial Macrophages Using Minimally Invasive Ultrasound-Guided Synovial Biopsies in Rheumatoid Arthritis. Arthritis Rheumatol 70:841-854
Fong, Lam-Kiu; Wang, Ziwei; Schatz, George C et al. (2018) The Role of Structural Enthalpy in Spherical Nucleic Acid Hybridization. J Am Chem Soc 140:6226-6230
Suraneni, Praveen K; Corey, Seth J; Hession, Michael J et al. (2018) Dynamins 2 and 3 control the migration of human megakaryocytes by regulating CXCR4 surface expression and ITGB1 activity. Blood Adv 2:3540-3552
Yan, M; Lewis, P L; Shah, R N (2018) Tailoring nanostructure and bioactivity of 3D-printable hydrogels with self-assemble peptides amphiphile (PA) for promoting bile duct formation. Biofabrication 10:035010
Edelbrock, Alexandra N; Àlvarez, Zaida; Simkin, Dina et al. (2018) Supramolecular Nanostructure Activates TrkB Receptor Signaling of Neuronal Cells by Mimicking Brain-Derived Neurotrophic Factor. Nano Lett 18:6237-6247
Fisher, Daniel W; Han, Ye; Lyman, Kyle A et al. (2018) HCN channels in the hippocampus regulate active coping behavior. J Neurochem 146:753-766

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