Abstract

The DNA Sequencing Shared Resource carries out centralized DNA sequencing for Cancer Center members. The availability of on-site DNA sequencing is of central importance to Cancer Center members. Local DNA sequencing allows for rapid turn-around, and with CCSG support, the cost is competitive with off-site commercial facilities. The DNA Sequencing Shared Resource contains an ABI 3700 automated capillary DNA sequencer, a Beckman DNA oligomer synthesizer, and a Robbins Scientific Hydra-96 microdispenser workstation. Computer support for the Shared Resource is provided from the adjoining laboratory of the core Director Moyzis. The DNA Sequencing Shared Resource sequences DNA samples that have been prepared by the investigators. The dried samples are processed by the Shared Resource and sequenced on the ABI 3700 sequencer. The sequence data is deposited as an FTP file on the Shared Resource website. Each investigator signs in and transfers the FTP file containing the sequencer output for his/her DNA samples. The automated and streamlined process allows for rapid and economic ($2.50 per sample) sequencing of DNA samples. The DNA sequencing Shared Resource will be moved to Sprague in the spring of 2002, in close proximity to the Microarray Shared Resource, and a bioinformatics facility. This will enhance the effectiveness of the Shared Resource.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA062203-09
Application #
6683021
Study Section
Subcommittee E - Prevention &Control (NCI)
Project Start
2002-09-19
Project End
2007-01-31
Budget Start
Budget End
Support Year
9
Fiscal Year
2002
Total Cost
Indirect Cost

  Institution

Name
University of California Irvine
Department
Type
DUNS #
161202122
City
Irvine
State
CA
Country
United States
Zip Code
92697

  Publications

McLelland, Bryce T; Lin, Bin; Mathur, Anuradha et al. (2018) Transplanted hESC-Derived Retina Organoid Sheets Differentiate, Integrate, and Improve Visual Function in Retinal Degenerate Rats. Invest Ophthalmol Vis Sci 59:2586-2603
Bota, Daniela A; Chung, Jinah; Dandekar, Manisha et al. (2018) Phase II study of ERC1671 plus bevacizumab versus bevacizumab plus placebo in recurrent glioblastoma: interim results and correlations with CD4+ T-lymphocyte counts. CNS Oncol 7:CNS22
Han, Han; Qi, Ruxi; Zhou, Jeff Jiajing et al. (2018) Regulation of the Hippo Pathway by Phosphatidic Acid-Mediated Lipid-Protein Interaction. Mol Cell 72:328-340.e8
Solares, Edwin A; Chakraborty, Mahul; Miller, Danny E et al. (2018) Rapid Low-Cost Assembly of the Drosophila melanogaster Reference Genome Using Low-Coverage, Long-Read Sequencing. G3 (Bethesda) 8:3143-3154
Wang, Yajun; Ngor, Arlene K; Nikoomanzar, Ali et al. (2018) Evolution of a General RNA-Cleaving FANA Enzyme. Nat Commun 9:5067
Qiu, Xiaolong; Lombardo, Jeremy A; Westerhof, Trisha M et al. (2018) Microfluidic filter device with nylon mesh membranes efficiently dissociates cell aggregates and digested tissue into single cells. Lab Chip 18:2776-2786
Patterson, Kurt; Yu, James; Landberg, Jenny et al. (2018) Functional genomics for the oleaginous yeast Yarrowia lipolytica. Metab Eng 48:184-196
Lee, J Scott; Roberts, Andrew; Juarez, Dennis et al. (2018) Statins enhance efficacy of venetoclax in blood cancers. Sci Transl Med 10:
Sierra, Robert A; Hoverter, Nathan P; Ramirez, Ricardo N et al. (2018) TCF7L1 suppresses primitive streak gene expression to support human embryonic stem cell pluripotency. Development 145:
Maciejewski, Sonia; Ullmer, Wendy; Semler, Bert L (2018) VPg unlinkase/TDP2 in cardiovirus infected cells: Re-localization and proteolytic cleavage. Virology 516:139-146

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