Abstract

Protein identification and proteomics are important new tools in cancer research. The Protein Mass Spectrometry Shared Resource will provide high-level mass spectrometry of biomolecules and protein analyses. This Shared Resource is based around a pair of tandem mass analyzers (the AB 4700 MALDITOF/ TOF and Thermo LTQ nanoESI-QIT), with access to as many as ten HPLC systems containing 10 quaternary and two binary pumps, seven PCs running at least 21 software packages, and numerous smaller items. The Facility is able to routinely identify proteins in slices from polyacryamide gels. In addition, it can routinely identify proteins in pull-down mixtures, complex virions, and whole cell extracts. They have done multi-dimensional (up to three dimensions) of tandem HPLC prior to LC-MALDI analysis. This capability includes two-dimensional protein fractionation (PF2D) and two and three dimensions of tandem peptide fractionation. This Facility provides an exciting springboard to the frontiers of state-of-the art proteomics. The Protein Mass Spectrometry Facility has been supported by the Cancer Center as a developing Shared Fesource for the past three years. Permanent funds are now requested for support as a full Shared Resource.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA062203-16
Application #
8215291
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2011-02-01
Budget End
2012-01-31
Support Year
16
Fiscal Year
2011
Total Cost
$20,689
Indirect Cost

  Institution

Name
University of California Irvine
Department
Type
DUNS #
046705849
City
Irvine
State
CA
Country
United States
Zip Code
92697

  Publications

McLelland, Bryce T; Lin, Bin; Mathur, Anuradha et al. (2018) Transplanted hESC-Derived Retina Organoid Sheets Differentiate, Integrate, and Improve Visual Function in Retinal Degenerate Rats. Invest Ophthalmol Vis Sci 59:2586-2603
Bota, Daniela A; Chung, Jinah; Dandekar, Manisha et al. (2018) Phase II study of ERC1671 plus bevacizumab versus bevacizumab plus placebo in recurrent glioblastoma: interim results and correlations with CD4+ T-lymphocyte counts. CNS Oncol 7:CNS22
Han, Han; Qi, Ruxi; Zhou, Jeff Jiajing et al. (2018) Regulation of the Hippo Pathway by Phosphatidic Acid-Mediated Lipid-Protein Interaction. Mol Cell 72:328-340.e8
Solares, Edwin A; Chakraborty, Mahul; Miller, Danny E et al. (2018) Rapid Low-Cost Assembly of the Drosophila melanogaster Reference Genome Using Low-Coverage, Long-Read Sequencing. G3 (Bethesda) 8:3143-3154
Wang, Yajun; Ngor, Arlene K; Nikoomanzar, Ali et al. (2018) Evolution of a General RNA-Cleaving FANA Enzyme. Nat Commun 9:5067
Qiu, Xiaolong; Lombardo, Jeremy A; Westerhof, Trisha M et al. (2018) Microfluidic filter device with nylon mesh membranes efficiently dissociates cell aggregates and digested tissue into single cells. Lab Chip 18:2776-2786
Patterson, Kurt; Yu, James; Landberg, Jenny et al. (2018) Functional genomics for the oleaginous yeast Yarrowia lipolytica. Metab Eng 48:184-196
Lee, J Scott; Roberts, Andrew; Juarez, Dennis et al. (2018) Statins enhance efficacy of venetoclax in blood cancers. Sci Transl Med 10:
Sierra, Robert A; Hoverter, Nathan P; Ramirez, Ricardo N et al. (2018) TCF7L1 suppresses primitive streak gene expression to support human embryonic stem cell pluripotency. Development 145:
Maciejewski, Sonia; Ullmer, Wendy; Semler, Bert L (2018) VPg unlinkase/TDP2 in cardiovirus infected cells: Re-localization and proteolytic cleavage. Virology 516:139-146

Showing the most recent 10 out of 1106 publications