The Transgenic Mouse Facility (TMF) shared resource communicates awareness about existing and emergingmethods and resources involving genetically modified mice to Cancer Center investigators and helps themincorporate these tools into their research programs. The TMF provides services on a recharge basis thatrequire specialized training, are technically difficult to perform, or require expensive equipment. The servicesinclude design, development, re-derivation, cryopreservation, and re-animation of genetically modified mice inan efficient and cost-effective manner. The TMF website (http://research.uci.edu/tmf/index.htm) listsinformation about new resources from the literature as well as available services including (a) consultation onstrategies to modify the mouse genome, (b) insertion of conventional multi-copy transgenes and bacterialartificial chromosomes (BAC) at random loci via pronuclear injection of DNA, (c) targeted insertion of singlecopytransgenes at the ROSA26 locus via homologous recombination in ES cells, (d) targeted mutagenesis ofendogenous loci in ES cells, (e) targeted mutagenesis of loci in mice via Cas-9, (f) cryopreservation of mousesperm and preimplantation-stage embryos, (g) importation, export, rederivation or reanimation of mousestrains via IVF or embryo transfer, (h) breeding and genotyping of mouse strains, (i) Southern blot analysis oftargeted loci in ES cells and animals. The TMF also collaborates with CFCCC members to develop or importnew methods involving genetically modified mice at no initial cost to the investigator. Consultation regardingprojects continues to be provided at no cost. In addition to CFCCC Members, the TMF supports investigatorsat many different universities and corporations within the USA. These include academic institutions (UCLA,UCSC, UCSB, UCR, UCSF, Stanford, USC, VA) and corporations (Irvine Scientific, Isis Pharmaceuticals,Peregrine Pharmaceuticals) in CA. The TMF also services investigators at institutions in 17 additional states,including 7 other NCI-designated CCCs: UCLA- Jonsson; UCSF - Diller; OHSU - Knight; MUSC - Hollings;Stanford Cancer Institute; St. Jude's Children's Research Center, USC-Norris. A link on the TMF home pageinvites investigators to contact the TMF Managing Director and Scientific Director to ask about resources fortheir use, including advice on mouse models of cancer, identifying and importing strains of mice with cancersusceptibility, and mouse genetics resources of use in studying the cancer problem. TMF personnelsubsequently identify resources to meet the researcher?s needs and provide these at the lowest possible cost.
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