BASIC RESEARCH GROUP CORE 004 ? BIOANALYTICS AND PROTEOMICS SHARED RESOURCE PROJECT SUMMARY/ABSTRACT The mission of the Bioanalytics & Proteomics Shared Resource (BPSR) is to provide cost-effective, state-of- the-art instrumentation and analytical expertise in mass spectrometry to investigators in the Vanderbilt-Ingram Cancer Center (VICC). The BPSR is centrally located on the Vanderbilt campus and is composed of three components that provide the following analytical services: 1) bioanalytical and drug metabolite, 2) analytical proteomics, and 3) molecular profiling and tissue imaging. The BPSR staff provides consultation on experimental design and sample preparation, as well as performs all aspects of mass spectrometry analyses and data analysis. LC-MS/MS, GC-MS and MALDI-TOF instruments are available for small molecule drug and metabolite analysis. LC-MS/MS and MALDI-TOF/TOF instrumentation are available for proteomics analysis, including MUDPIT, SILAC and iTRAQ. A variety of MALDI-based instruments are provided for molecular profiling and tissue imaging experiments. In addition, ICP-MS capabilities are offered for elemental analysis and tissue imaging. Specific services include identification and quantification of small molecules in biofluids and tissues, identification and quantification of proteins and their modifications, and imaging of small molecules and protein in tissues using imaging mass spectrometry. The BPSR staff provides education and training in sample preparation, instrumental analysis and data analysis to VICC investigators and their laboratory personnel. Lastly, the BPSR collaborates with VICC proteomics experts to continue to offer the latest cutting- edge technology and methods in bimolecular mass spectrometry analysis for high-impact cancer discovery.

Public Health Relevance

BASIC RESEARCH GROUP CORE 004 ? BIOANALYTICAL AND PROTEOMICS SHARED RESOURCE PROJECT NARRATIVE Per the PAR-13-386 FOA, the project narrative is not applicable for the Bioanalytical and Proteomics Shared Resource.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA068485-21
Application #
9152299
Study Section
Subcommittee A - Cancer Centers (NCI-A)
Program Officer
Marino, Michael A
Project Start
Project End
Budget Start
2016-09-01
Budget End
2017-08-31
Support Year
21
Fiscal Year
2016
Total Cost
$348,178
Indirect Cost
$126,409
Name
Vanderbilt University Medical Center
Department
Type
DUNS #
079917897
City
Nashville
State
TN
Country
United States
Zip Code
37232
Saito-Diaz, Kenyi; Benchabane, Hassina; Tiwari, Ajit et al. (2018) APC Inhibits Ligand-Independent Wnt Signaling by the Clathrin Endocytic Pathway. Dev Cell 44:566-581.e8
Cardin, Dana B; Thota, Ramya; Goff, Laura W et al. (2018) A Phase II Study of Ganetespib as Second-line or Third-line Therapy for Metastatic Pancreatic Cancer. Am J Clin Oncol 41:772-776
Bloodworth, Melissa H; Rusznak, Mark; Pfister, Connor C et al. (2018) Glucagon-like peptide 1 receptor signaling attenuates respiratory syncytial virus-induced type 2 responses and immunopathology. J Allergy Clin Immunol 142:683-687.e12
Dutter, Brendan F; Ender, Anna; Sulikowski, Gary A et al. (2018) Rhodol-based thallium sensors for cellular imaging of potassium channel activity. Org Biomol Chem 16:5575-5579
Hormuth 2nd, David A; Weis, Jared A; Barnes, Stephanie L et al. (2018) Biophysical Modeling of In Vivo Glioma Response After Whole-Brain Radiation Therapy in a Murine Model of Brain Cancer. Int J Radiat Oncol Biol Phys 100:1270-1279
Rojas, Juan D; Lin, Fanglue; Chiang, Yun-Chen et al. (2018) Ultrasound Molecular Imaging of VEGFR-2 in Clear-Cell Renal Cell Carcinoma Tracks Disease Response to Antiangiogenic and Notch-Inhibition Therapy. Theranostics 8:141-155
Lewis Jr, James S; Shelton, Jeremy; Kuhs, Krystle Lang et al. (2018) p16 Immunohistochemistry in Oropharyngeal Squamous Cell Carcinoma Using the E6H4 Antibody Clone: A Technical Method Study for Optimal Dilution. Head Neck Pathol 12:440-447
Vierra, Nicholas C; Dickerson, Matthew T; Jordan, Kelli L et al. (2018) TALK-1 reduces delta-cell endoplasmic reticulum and cytoplasmic calcium levels limiting somatostatin secretion. Mol Metab 9:84-97
Schlegel, Cameron; Weis, Victoria G; Knowles, Byron C et al. (2018) Apical Membrane Alterations in Non-intestinal Organs in Microvillus Inclusion Disease. Dig Dis Sci 63:356-365
Piñeros, Marion; Frech, Silvina; Frazier, Lindsay et al. (2018) Advancing Reliable Data for Cancer Control in the Central America Four Region. J Glob Oncol :1-11

Showing the most recent 10 out of 2462 publications