Advanced Multiscale Microscopy

Kaech Petrie, Stefanie

Abstract

ADVANCED MULTISCALE MICROSCOPY SHARED RESOURCE (AMMSR) Co-Directors: Stefanie Kaech Petrie, PhD, and Heidi Feiler, PhD ABSTRACT / PROJECT SUMMARY The Advanced Multiscale Microscopy Shared Resource (AMMSR) delivers access to cutting-edge imaging instrumentation and comprehensive technical support for cellular, subcellular and molecular imaging to Knight Cancer Institute (Knight) members. The AMMSR integrates light microscopy and electron microscopy (EM) services and expertise to provide comprehensive support for analysis of samples across the full microscopy resolution spectrum. The AMMSR provides Knight Investigators: (1) coordinated access to advanced, multiscale imaging technologies, (2) expert guidance, advice and training on sample preparation and the use of light microscopy, EM, and correlative light and electron microscopy (CLEM) platforms, (3) optimized workflows, (4) access to multidisciplinary faculty experts with expertise in physics, biomedical engineering, chemistry, cancer biology and computation that provide workflow development support for specific AMMSR instruments, (5) access to a laboratory where users can receive training, or conduct, EM sample preparation, (6) comprehensive services provided by skilled core staff for EM sample preparation and evaluation, optical and EM image and data acquisition, and computational analysis, (7) access to analysis stations and software specialized for optical and EM imaging analysis, (8) access to formal workshops and educational opportunities aimed at increasing general awareness about standard and innovative microscopy technologies, and their successful application in basic and translational cancer research. Recent developments in imaging technologies, including super-resolution microscopy, larger volume 3D light microscopy EM approaches, and CLEM are providing new resources for basic and translational research. These imaging technologies are essential tools in cancer research, leading to a better understanding of cancer mechanisms and therapeutic resistance, as well as discovering new targets for drug development. The comprehensive support and extensive expertise offered through the AMMSR provides Knight Investigators with the means to understand cancer at the cellular and organismal level.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Cancer Institute (NCI)
• Type: Center Core Grants (P30)
• Project #: 2P30CA069533-19
• Application #: 9278523
• Study Section: Subcommittee I - Transistion to Independence (NCI)
• Budget Start: 2017-07-01
• Budget End: 2018-06-30
• Support Year: 19
• Fiscal Year: 2017

Institution

• Name: Oregon Health and Science University
• DUNS #: 096997515
• City: Portland
• State: OR
• Country: United States
• Zip Code: 97239

Publications

Lane, Ryan S; Femel, Julia; Breazeale, Alec P et al. (2018) IFN?-activated dermal lymphatic vessels inhibit cytotoxic T cells in melanoma and inflamed skin. J Exp Med 215:3057-3074

Smith, Nicholas R; Swain, John R; Davies, Paige S et al. (2018) Monoclonal Antibodies Reveal Dynamic Plasticity Between Lgr5- and Bmi1-Expressing Intestinal Cell Populations. Cell Mol Gastroenterol Hepatol 6:79-96

Langer, E M; Kendsersky, N D; Daniel, C J et al. (2018) ZEB1-repressed microRNAs inhibit autocrine signaling that promotes vascular mimicry of breast cancer cells. Oncogene 37:1005-1019

Sorace, Anna G; Partridge, Savannah C; Li, Xia et al. (2018) Distinguishing benign and malignant breast tumors: preliminary comparison of kinetic modeling approaches using multi-institutional dynamic contrast-enhanced MRI data from the International Breast MR Consortium 6883 trial. J Med Imaging (Bellingham) 5:011019

Medler, Terry R; Murugan, Dhaarini; Horton, Wesley et al. (2018) Complement C5a Fosters Squamous Carcinogenesis and Limits T Cell Response to Chemotherapy. Cancer Cell 34:561-578.e6

Kelley, Katherine A; Wieghard, Nicole; Chin, Yuki et al. (2018) MiR-486-5p Downregulation Marks an Early Event in Colorectal Carcinogenesis. Dis Colon Rectum 61:1290-1296

Davare, Monika A; Henderson, Jacob J; Agarwal, Anupriya et al. (2018) Rare but Recurrent ROS1 Fusions Resulting From Chromosome 6q22 Microdeletions are Targetable Oncogenes in Glioma. Clin Cancer Res 24:6471-6482

Kurtz, Stephen E; Eide, Christopher A; Kaempf, Andy et al. (2018) Dual inhibition of JAK1/2 kinases and BCL2: a promising therapeutic strategy for acute myeloid leukemia. Leukemia 32:2025-2028

Sehrawat, Archana; Gao, Lina; Wang, Yuliang et al. (2018) LSD1 activates a lethal prostate cancer gene network independently of its demethylase function. Proc Natl Acad Sci U S A 115:E4179-E4188

Watson, Spencer S; Dane, Mark; Chin, Koei et al. (2018) Microenvironment-Mediated Mechanisms of Resistance to HER2 Inhibitors Differ between HER2+ Breast Cancer Subtypes. Cell Syst 6:329-342.e6