) The Flow Cytometry Core Facility at Moffitt Cancer Center provides a centralized service for Cancer Center members and University of South Florida faculty with cancer related research interests. Flow cytometry is a critical technical aspect of numerous Cancer Center projects due to its versatility and usefulness for rapid analysis of multiple characteristics on many cells, as well as for the identification of different cell types in mixed populations. Flow cytometry is a diverse analytical tool, which is crucial in the clinical and basic research pursuits to understand cancer biology and control. The Core provides the following services: single and multicolor fluorescence analysis, cell sorting, highspeed sorting, DNA content and cell cycle analysis, cytoplasmic calcium determinations, viability and apoptosis assessments, data analysis and graphics, training of investigators for data acquisition and analysis. The Facility provides expert technical consultation on experimental designs and trouble-shooting, sample acquisition and analysis. In 1999 the facility was utilized by 21 funded investigators (28 projects) which accounted for 87 percent of the total usage. Usage has increased steadily over the last several years and is projected to increase due to the addition of a high-speed sorter, recruitment of researchers who utilize flow cytometry, and the addition of a staff scientist within the Core. The funds requested from the CCSG will support a portion of two FTEs and 40 percent of the instrument service contracts. The total operating cost of this facility is $210,462. We are requesting $75,396 from the CCSG allocation, an amount that represents approximately 36 percent of the total operating budget.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
3P30CA076292-05S1
Application #
6654051
Study Section
Project Start
2002-02-01
Project End
2003-01-31
Budget Start
Budget End
Support Year
5
Fiscal Year
2002
Total Cost
Indirect Cost
Name
University of South Florida
Department
Type
DUNS #
City
Tampa
State
FL
Country
United States
Zip Code
33612
Simmons, Vani N; Sutton, Steven K; Meltzer, Lauren R et al. (2018) Long-term outcomes from a self-help smoking cessation randomized controlled trial. Psychol Addict Behav 32:710-714
Dai, Juncheng; Li, Zhihua; Amos, Christopher I et al. (2018) Systematic analyses of regulatory variants in DNase I hypersensitive sites identified two novel lung cancer susceptibility loci. Carcinogenesis :
Cherezov, Dmitry; Hawkins, Samuel H; Goldgof, Dmitry B et al. (2018) Delta radiomic features improve prediction for lung cancer incidence: A nested case-control analysis of the National Lung Screening Trial. Cancer Med 7:6340-6356
Neumeyer, Sonja; Banbury, Barbara L; Arndt, Volker et al. (2018) Mendelian randomisation study of age at menarche and age at menopause and the risk of colorectal cancer. Br J Cancer 118:1639-1647
Wheldon, Christopher W; Schabath, Matthew B; Hudson, Janella et al. (2018) Culturally Competent Care for Sexual and Gender Minority Patients at National Cancer Institute-Designated Comprehensive Cancer Centers. LGBT Health 5:203-211
Lin, Hui-Yi; Huang, Po-Yu; Chen, Dung-Tsa et al. (2018) AA9int: SNP interaction pattern search using non-hierarchical additive model set. Bioinformatics 34:4141-4150
Palmer, Amanda M; Brandon, Thomas H (2018) How do electronic cigarettes affect cravings to smoke or vape? Parsing the influences of nicotine and expectancies using the balanced-placebo design. J Consult Clin Psychol 86:486-491
Hellmann, Matthew D; Callahan, Margaret K; Awad, Mark M et al. (2018) Tumor Mutational Burden and Efficacy of Nivolumab Monotherapy and in Combination with Ipilimumab in Small-Cell Lung Cancer. Cancer Cell 33:853-861.e4
Trabert, Britton; Poole, Elizabeth M; White, Emily et al. (2018) Analgesic Use and Ovarian Cancer Risk: An Analysis in the Ovarian Cancer Cohort Consortium. J Natl Cancer Inst :
Liu, Ying; Kim, Jongphil; Balagurunathan, Yoganand et al. (2018) Prediction of pathological nodal involvement by CT-based Radiomic features of the primary tumor in patients with clinically node-negative peripheral lung adenocarcinomas. Med Phys 45:2518-2526

Showing the most recent 10 out of 1254 publications