The Flow Cytometry Core Facility (FCCF) at the Moffitt Cancer Center was established in 1990 to provide a centralized flow cytometry service for Cancer Center members. Flow cytometry is an indispensable analytical tool for cell biology, immunology and translational research efforts at the Cancer Center. This technology provides a means for rapidly and accurately analyzing multiple characteristics of biological particles, as well as an ability to rapidly isolate and purify cell populations of interest for the purpose of assessing immunological functions and molecular distinctions, cell culture cloning and animal transplantation studies. The FCCF is staffed with highly trained instrument specialists with a combined 36 years of research-based flow cytometry experience. The facility houses six bench-top analyzers, two cell sorters, a bead array analyzer, an automated bead sorter and a fluorescent microscope. This includes the most recently added BD Biosciences customized LSR II analyzer, which was added in response to increased demand for sophisticated polychromatic analyses and high throughput capability. Its customized optical bench has expanded services to the Moffitt investigators doing novel, cutting-edge cancer biology studies. Fifty-six funded members routinely access the FCCF, which is up from thirty-nine in the 2006 review period. In 2008, the FCCF began to use the Laboratory Information Management System (LIMS) to consolidate usage tracking, scheduling, and billing. Flow LIMS also provides a secure repository for Core project data that is accessible by the Pl and the laboratory staff. The FCCF provides critical support for the scientific programs at the Cancer Center by providing investigators with access to state-of-the-art instrumentation, cutting-edge cytometry applications and an exceptionally well trained staff. Overall usage in the FCCF has increased over 30% since the 2006 CCSG submission. The Core requests CCSG Support of $57,049, which is 24% of its operational budget. Over 93% of usage is by Moffitt members and peer-reviewed.

Public Health Relevance

The FCCF provides Moffitt Members a high-quality, state-of-the-art facility that has consistently met investigators'demands for flow cytometry. The core provides researchers with a well-managed, cost-effective facility that functions as a repository for centralized technical expertise, sophisticated instrumentation and software, as well as educational and training opportunities.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
Project #
Application #
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
H. Lee Moffitt Cancer Center & Research Institute
United States
Zip Code
Hoffman, Melissa A; Fang, Bin; Haura, Eric B et al. (2018) Comparison of Quantitative Mass Spectrometry Platforms for Monitoring Kinase ATP Probe Uptake in Lung Cancer. J Proteome Res 17:63-75
Kahen, Elliot John; Brohl, Andrew; Yu, Diana et al. (2018) Neurofibromin level directs RAS pathway signaling and mediates sensitivity to targeted agents in malignant peripheral nerve sheath tumors. Oncotarget 9:22571-22585
Gonzalez, Brian D; Small, Brent J; Cases, Mallory G et al. (2018) Sleep disturbance in men receiving androgen deprivation therapy for prostate cancer: The role of hot flashes and nocturia. Cancer 124:499-506
Puri, Sonam; Hyland, Kelly A; Weiss, Kristine Crowe et al. (2018) Prediction of chemotherapy-induced nausea and vomiting from patient-reported and genetic risk factors. Support Care Cancer 26:2911-2918
Lu, Yingchang; Beeghly-Fadiel, Alicia; Wu, Lang et al. (2018) A Transcriptome-Wide Association Study Among 97,898 Women to Identify Candidate Susceptibility Genes for Epithelial Ovarian Cancer Risk. Cancer Res 78:5419-5430
Eroglu, Zeynep; Zaretsky, Jesse M; Hu-Lieskovan, Siwen et al. (2018) High response rate to PD-1 blockade in desmoplastic melanomas. Nature 553:347-350
Kasting, Monica L; Christy, Shannon M; Sutton, Steven K et al. (2018) Florida physicians' reported use of AFIX-based strategies for human papillomavirus vaccination. Prev Med 116:143-149
Phadke, Manali; Remsing Rix, Lily L; Smalley, Inna et al. (2018) Dabrafenib inhibits the growth of BRAF-WT cancers through CDK16 and NEK9 inhibition. Mol Oncol 12:74-88
Park, Jae H; Rivière, Isabelle; Gonen, Mithat et al. (2018) Long-Term Follow-up of CD19 CAR Therapy in Acute Lymphoblastic Leukemia. N Engl J Med 378:449-459
Jiang, Kun; Neill, Kevin; Cowden, Daniel et al. (2018) Expression of CAS/CSE1L, the Cellular Apoptosis Susceptibility Protein, Correlates With Neoplastic Progression in Barrett's Esophagus. Appl Immunohistochem Mol Morphol 26:552-556

Showing the most recent 10 out of 1254 publications