The Molecular Genomics Core Facility provides a centralized resource for the molecular analysis of the genome and transcriptome of cells and tissues. Analysis can occur at the level of a single nucleotide, a single molecule, and a single sample or involve multiple nucleotides, multiple molecules, and multiple samples. The laboratory offers single molecule sequencing, PCR-based analysis of genes and transcripts, and microarray based analysis of transcriptomes and genomes. In this era of high content analysis the goal is to economically measure the highest combination of nucleotides, molecules, and samples. As a centralized facility for all levels of assessment the laboratory provides the expertise required to determine the best technology to use for a research objective. The laboratory also provides expertise in the isolation, preservation, and handling of nucleic acids and training in the use of basic bioinformatics software needed to assess experimental data, access web resources, or perform follow-up investigations. Molecular Genomics Core represents the integration of complementary platforms through a single access point. The core merges the Microarray Core and the Molecular Biology Core as submitted at the last renewal. New equipment and services since the last renewal includes: an ABI 7900 Real Time PCR machine, a Agilent Surescan High-resolution DNA microarray scanner, an Xceed Molecular Ziplex microarray system, the lllumina HiScanSQ system, microarray-based for ChlP-on-ChIP experiements, expanded genotyping and mutational analysis options, and implementation of a LIMS system. The Core requests CCSG Support of $264,488, which is 30% of its operational budget. Over 92% of usage is by Moffitt members and peer-reviewed.
The core provides an efficient and essential resource for Moffitt Members to analyze changes in gene/genomes and epigenetie factors. The Molecular Genomics Core has contributed greatly to the scientific investigations occurring at the Cancer Center and contributed to science on a national level.
|Liu, Ying; Wang, Hua; Li, Qian et al. (2018) Radiologic Features of Small Pulmonary Nodules and Lung Cancer Risk in the National Lung Screening Trial: A Nested Case-Control Study. Radiology 286:298-306|
|Padron, Eric; Ball, Markus C; Teer, Jamie K et al. (2018) Germ line tissues for optimal detection of somatic variants in myelodysplastic syndromes. Blood 131:2402-2405|
|Karolak, Aleksandra; Markov, Dmitry A; McCawley, Lisa J et al. (2018) Towards personalized computational oncology: from spatial models of tumour spheroids, to organoids, to tissues. J R Soc Interface 15:|
|Lee, Morgan S; Tyson, Dinorah Martinez; Gonzalez, Brian D et al. (2018) Anxiety and depression in Spanish-speaking Latina cancer patients prior to starting chemotherapy. Psychooncology 27:333-338|
|Correa, John B; Brandon, Karen O; Meltzer, Lauren R et al. (2018) Electronic cigarette use among patients with cancer: Reasons for use, beliefs, and patient-provider communication. Psychooncology 27:1757-1764|
|Verduzco, Daniel; Kuenzi, Brent M; Kinose, Fumi et al. (2018) Ceritinib Enhances the Efficacy of Trametinib in BRAF/NRAS-Wild-Type Melanoma Cell Lines. Mol Cancer Ther 17:73-83|
|Divakaran, Anand; Talluri, Siva K; Ayoub, Alex M et al. (2018) Molecular Basis for the N-Terminal Bromodomain-and-Extra-Terminal-Family Selectivity of a Dual Kinase-Bromodomain Inhibitor. J Med Chem 61:9316-9334|
|de Mingo Pulido, Álvaro; Gardner, Alycia; Hiebler, Shandi et al. (2018) TIM-3 Regulates CD103+ Dendritic Cell Function and Response to Chemotherapy in Breast Cancer. Cancer Cell 33:60-74.e6|
|McIntyre, Jessica; Jiménez, Julio; Rivera, Yonaira M et al. (2018) Comparison of Health Communication Channels for Reaching Hispanics About Biobanking: a Pilot Trial. J Cancer Educ 33:833-841|
|Li, Gongbo; Boucher, Justin C; Kotani, Hiroshi et al. (2018) 4-1BB enhancement of CAR T function requires NF-?B and TRAFs. JCI Insight 3:|
Showing the most recent 10 out of 1254 publications