The Analytic Microscopy Core Facility (AMC) was established in 1999 to provide investigators with the tools and technical expertise required for acquisition and analysis of advanced optical microscopy applications. Optical microscopy offers investigators the ability to visualize and quantify complex cellular and sub-cellular level processes in multiple dimensions. The core's goal is to provide expert training, experimental planning, hands-on assistance, coupled with state-of-the-art instrumentation and responsible equipment maintenance. AMC is well equipped, skillfully staffed, heavily used and has proven to be an invaluable resource for Cancer Center researchers interested in the most demanding projects. The core seeks to continue to identify and obtain emerging technologies that meet the needs of Moffitt Members. Since the last renewal, the AMC has continued to experience a dramatic increase in usage, which has prompted the growth of services, instrumentation, and expert personnel. Since 2006, the Core has added five major instrument systems and upgraded four older existing systems. New equipment include Aperio's ScanScope XT slide scanning technology to include a higher throughput and more advanced analysis system also capable of fluorescent imaging;a state-of-the-art Leica SPS AOBS tandem laser scanning confocal microscope;a fully automated Zeiss Observer wide-field fluorescence inverted microscope;and a cluster of stand alone offline image analysis workstations. In addition to added instrumentation, the AMC hired an additional Microscopy Specialist 111 (Joseph Johnson in 2006) to supplement the Core Staff Scientist (Mark Lloyd in 2003) and Microscopy Specialist II (Nancy Burke in 2005). The Core requests CCSG Support of $109,212, which is 34% of its operational budget. Over 70% of usage is by Moffitt members and peer-reviewed cancer investigators.

Public Health Relevance

The Analytic Microscopy Core is responsive to the science performed at the Cancer Center and committed to the acquisition of state-of-the-art instrumentation that is easy to use and that expands microscopy capabilities to meet the needs of Moffitt's members. This approach allows members to focus on their science and leaves the expertise, maintenance, and support of high-end microscopy equipment to a highly trained and dedicated full-time staff.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
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Subcommittee G - Education (NCI)
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H. Lee Moffitt Cancer Center & Research Institute
United States
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Schaal, Courtney M; Bora-Singhal, Namrata; Kumar, Durairaj Mohan et al. (2018) Regulation of Sox2 and stemness by nicotine and electronic-cigarettes in non-small cell lung cancer. Mol Cancer 17:149
Zhu, Genyuan; Brayer, Jason; Padron, Eric et al. (2018) OMIP-049: Analysis of Human Myelopoiesis and Myeloid Neoplasms. Cytometry A 93:982-986
Bowman-Curci, Meghan; Quinn, Gwendolyn P; Reinecke, Joyce et al. (2018) Comparing fertility preservation resources and policies between NCCN member and non-member institutions. Support Care Cancer :
Hampras, Shalaka S; Locke, Frederick L; Chavez, Julio C et al. (2018) Prevalence of cutaneous viral infections in incident cutaneous squamous cell carcinoma detected among chronic lymphocytic leukemia and hematopoietic stem cell transplant patients. Leuk Lymphoma 59:911-917
Kim, Youngchul; Pierce, Christine M; Robinson, Lary A (2018) Impact of viral presence in tumor on gene expression in non-small cell lung cancer. BMC Cancer 18:843
Persi, Erez; Duran-Frigola, Miquel; Damaghi, Mehdi et al. (2018) Systems analysis of intracellular pH vulnerabilities for cancer therapy. Nat Commun 9:2997
Rosenberger, Albert; Hung, Rayjean J; Christiani, David C et al. (2018) Genetic modifiers of radon-induced lung cancer risk: a genome-wide interaction study in former uranium miners. Int Arch Occup Environ Health 91:937-950
Chen, Yan; Zhu, Jin-Yi; Hong, Kwon Ho et al. (2018) Structural Basis of ALDH1A2 Inhibition by Irreversible and Reversible Small Molecule Inhibitors. ACS Chem Biol 13:582-590
Kahen, Elliot John; Brohl, Andrew; Yu, Diana et al. (2018) Neurofibromin level directs RAS pathway signaling and mediates sensitivity to targeted agents in malignant peripheral nerve sheath tumors. Oncotarget 9:22571-22585
Hoffman, Melissa A; Fang, Bin; Haura, Eric B et al. (2018) Comparison of Quantitative Mass Spectrometry Platforms for Monitoring Kinase ATP Probe Uptake in Lung Cancer. J Proteome Res 17:63-75

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