The Mouse Genetics Laboratory (MGL) Shared Resource provides Masonic Cancer Center (MCC) members with access to state-of-the-art technologies required to create and efficiently study genetically modified mice. Genetically modified mice have been vital tools for cancer studies for many years, enabling researchers to determine the roles of specific genes in cancer-relevant traits such as immune evasion, tumor initiation, and metastasis. MGL produces transgenic and knockout mice, cryopreserves mouse sperm and embryos, performs embryonic stem cell gene targeting, assists with mouse embryo manipulation, and provides scientific consultation. These services provide MCC members convenient, cost-effective access to genetically modified mice. Access to these mice and associated technologies is critical to the ongoing work of the MCC, including members of the Genetic Mechanisms of Cancer, Immunology, and Cell Signaling Programs. Transgenic mice produced by this shared resource have been used to create a system for performing transposon-based forward genetic screens for cancer-associated genes in hepatocellular carcinoma, glioma, T cell leukemia, and colorectal cancer, among others. Other transgenic mice have been used to produce models of B cell malignancy, breast cancer, and other tumor types, and to study aspects of T cell development and immunity. These studies have relevance for understanding tumor surveillance and methods to break tolerance for antitumor immunity. MCC members have also studied the DMRT family of transcriptional regulators and the interaction between loss of developmental control and development of testicular tumors. MGL is co-directed by David Largaespada, PhD who has 24 years of experience with mouse biomedical research, and Anindya Bagchi, PhD who has more than 10 years of experience. Ms. Sandra Wagner has overseen the day-to-day operations of MGL for 15 years and educates users of the laboratory on policies, protocols, and maintenance of transgenic mouse strains. The MGL has served the MCC for 15 years, since the MCC was established. The value of MGL has been recognized by the University community with a need to accommodate work not related to cancer. During the last period of support, the Academic Health Center has become the administrative home of the MGL. We anticipate that this Shared Resource will continue to serve investigators who require in vivo genetic confirmation of important hypotheses involving many aspects of cancer biology.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
Project #
Application #
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Minnesota Twin Cities
United States
Zip Code
Lin, Lifeng; Chu, Haitao; Murad, Mohammad Hassan et al. (2018) Empirical Comparison of Publication Bias Tests in Meta-Analysis. J Gen Intern Med 33:1260-1267
Rashidi, Armin; Ebadi, Maryam; Said, Bassil et al. (2018) Absence of early HHV-6 reactivation after cord blood allograft predicts powerful graft-versus-tumor effect. Am J Hematol :
Bejanyan, Nelli; Brunstein, Claudio G; Cao, Qing et al. (2018) Delayed immune reconstitution after allogeneic transplantation increases the risks of mortality and chronic GVHD. Blood Adv 2:909-922
Bachanova, Veronika; Sarhan, Dhifaf; DeFor, Todd E et al. (2018) Haploidentical natural killer cells induce remissions in non-Hodgkin lymphoma patients with low levels of immune-suppressor cells. Cancer Immunol Immunother 67:483-494
Hupp, Meghan; Williams, Sarah; Dunnette, Brian et al. (2018) Comparison of evaluation techniques, including digital image analysis, for MYC protein expression by immunohistochemical stain in aggressive B-cell lymphomas. Hum Pathol :
Rashidi, Armin; Shanley, Ryan; Holtan, Shernan G et al. (2018) Pretransplant Serum Citrulline Predicts Acute Graft-versus-Host Disease. Biol Blood Marrow Transplant 24:2190-2196
Ma, Bin; Zarth, Adam T; Carlson, Erik S et al. (2018) Methyl DNA Phosphate Adduct Formation in Rats Treated Chronically with 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone and Enantiomers of Its Metabolite 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanol. Chem Res Toxicol 31:48-57
Hatsukami, Dorothy K; Luo, Xianghua; Jensen, Joni A et al. (2018) Effect of Immediate vs Gradual Reduction in Nicotine Content of Cigarettes on Biomarkers of Smoke Exposure: A Randomized Clinical Trial. JAMA 320:880-891
Lee, Hak Rae; Leslie, Faith; Azarin, Samira M (2018) A facile in vitro platform to study cancer cell dormancy under hypoxic microenvironments using CoCl2. J Biol Eng 12:12
Yang, Libang; Herrera, Jeremy; Gilbertsen, Adam et al. (2018) IL-8 mediates idiopathic pulmonary fibrosis mesenchymal progenitor cell fibrogenicity. Am J Physiol Lung Cell Mol Physiol 314:L127-L136

Showing the most recent 10 out of 1013 publications