Abstract

, Proteomics Core (PC) The Indiana University School of Medicine (IUSM) Proteomics Core (PC) is a mass spectrometry (MS)-centric protein analysis center with extensive expertise in affinity-purification mass spectrometry methods and quantitative proteomics. IUSM has recently purchased two state-of-the-art mass spectrometers, two ultra high- pressure liquid chromatography systems, and a variety of ionization sources to facilitate innovative application of proteomics methods to basic and clinical research questions for all researchers at IUSM, particularly IU Simon Cancer Center (IUSCC) members. Our central goal is to apply and develop state-of-the-art proteomics methods to measure system-wide changes in protein biology, as it relates to cancer biological questions. The proteomics core and IUSCC leadership have developed the following specific aims to meet the critical needs of IUSCC members for proteomics services:
Specific Aim 1 : To provide IUSCC users with state-of-the art proteomics services with a focus on global proteome and phosphoproteome profiling;
Specific Aim 2 : To develop leading edge proteomics services in chemoproteomics to facilitate drug discovery and drug characterization in IUSCC;
Specific Aim 3 : To expand affinity-purification MS analysis for identification of high-confidence interacting proteins;
and Specific Aim 4 : To develop, provide, and/or organize educational opportunities related to proteomics technologies, experimental design and data analysis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA082709-20
Application #
9793159
Study Section
Subcommittee I - Transistion to Independence (NCI)
Project Start
Project End
Budget Start
2019-09-01
Budget End
2020-08-31
Support Year
20
Fiscal Year
2019
Total Cost
Indirect Cost

  Institution

Name
Indiana University-Purdue University at Indianapolis
Department
Type
DUNS #
603007902
City
Indianapolis
State
IN
Country
United States
Zip Code
46202

  Publications

Hoggatt, Jonathan; Singh, Pratibha; Tate, Tiffany A et al. (2018) Rapid Mobilization Reveals a Highly Engraftable Hematopoietic Stem Cell. Cell 172:191-204.e10
Filley, Anna; Henriquez, Mario; Bhowmik, Tanmoy et al. (2018) Immunologic and gene expression profiles of spontaneous canine oligodendrogliomas. J Neurooncol 137:469-479
Sishtla, Kamakshi; Pitt, Natalie; Shadmand, Mehdi et al. (2018) Observations on spontaneous tumor formation in mice overexpressing mitotic kinesin Kif14. Sci Rep 8:16152
Koh, Byunghee; Abdul Qayum, Amina; Srivastava, Rajneesh et al. (2018) A conserved enhancer regulates Il9 expression in multiple lineages. Nat Commun 9:4803
Reese, Michael J; Knapp, Deborah W; Anderson, Kimberly M et al. (2018) In vitro effect of chlorambucil on human glioma cell lines (SF767 and U87-MG), and human microvascular endothelial cell (HMVEC) and endothelial progenitor cells (ECFCs), in the context of plasma chlorambucil concentrations in tumor-bearing dogs. PLoS One 13:e0203517
Singh, Pratibha; Fukuda, Seiji; Liu, Liqiong et al. (2018) Survivin Is Required for Mouse and Human Bone Marrow Mesenchymal Stromal Cell Function. Stem Cells 36:123-129
Olivos 3rd, David J; Perrien, Daniel S; Hooker, Adam et al. (2018) The proto-oncogene function of Mdm2 in bone. J Cell Biochem 119:8830-8840
Shiue, Kevin; Cerra-Franco, Alberto; Shapiro, Ronald et al. (2018) Histology, Tumor Volume, and Radiation Dose Predict Outcomes in NSCLC Patients After Stereotactic Ablative Radiotherapy. J Thorac Oncol 13:1549-1559
Liu, Yunhua; Xu, Hanchen; Van der Jeught, Kevin et al. (2018) Somatic mutation of the cohesin complex subunit confers therapeutic vulnerabilities in cancer. J Clin Invest 128:2951-2965
Pin, Fabrizio; Barreto, Rafael; Kitase, Yukiko et al. (2018) Growth of ovarian cancer xenografts causes loss of muscle and bone mass: a new model for the study of cancer cachexia. J Cachexia Sarcopenia Muscle 9:685-700

Showing the most recent 10 out of 256 publications