The Optical Biology Shared Resource (Opticore) provides access to state-of-the-art flow cytometry, cell sorting, and laser scanning cytometry for the UC Davis Cancer Center community. The core facility is located on the Davis campus with two satellites in Sacramento. The objectives of this shared resource are to: provide state-of-the-art cell sorting and analytical cytometry;provide consultation about appropriate experimental design and data analysis;provide education about routine as well as cutting-edge cytometry applications;facilitate interactions among researchers using these techniques;and stimulate development of new approaches for cancer research. This shared resource provides cost-effective services and training. Investigators and their laboratory members work closely with the Technical Director to learn to use multicolor benchtop analyzers. The staff teaches an annual summer flow cytometry course for new users. Multi-laser, high-speed cell sorters are available on both campuses and are operated by Opticore staff. A biohazard containment system permits sorting of unfixed cells. The 3-laser scanning cytometer is configured for multiparameter and high-throughput applications, including analysis of tissue microarrays. These instruments have been upgraded with institutional support and are maintained through ongoing service contracts. The Cancer Center administration has designed and implemented a web-based reservation and reporting system. Dr. Barbara Shacklett is the Scientific Director;the Technical Director, Ms. Oxford, has developed notable expertise in multicolor analysis and high speed sorting. She is assisted by a Technical Manager, Ms. McLaughlin, who supervises operations in Sacramento. An Advisory Committee meets at least once a year to review operations, set policy, and make plans. Since the last submission, the user pool has expanded and Cancer Center members from all six research programs have used this shared resource. Members currently account for over 80% of its use, generating results published in high-impact journals and submitted in grant applications.

Public Health Relevance

The Optical Biology Shared Resource (Opticore) provides access to state-of-the-art flow cytometry, cell sorting, and laser scanning cytometry for the UC Davis Cancer Center research community. This technology is widely used in cancer research to evaluate cells and cell properties, and thus contributes to improved cancer research, and ultimately to better cancer therapies.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA093373-12
Application #
8743646
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2014-07-01
Budget End
2015-06-30
Support Year
12
Fiscal Year
2014
Total Cost
$94,558
Indirect Cost
$32,952
Name
University of California Davis
Department
Type
DUNS #
047120084
City
Davis
State
CA
Country
United States
Zip Code
95618
Braithwaite, Dejana; Miglioretti, Diana L; Zhu, Weiwei et al. (2018) Family History and Breast Cancer Risk Among Older Women in the Breast Cancer Surveillance Consortium Cohort. JAMA Intern Med 178:494-501
Unger, Jakob; Sun, Tianchen; Chen, Yi-Ling et al. (2018) Method for accurate registration of tissue autofluorescence imaging data with corresponding histology: a means for enhanced tumor margin assessment. J Biomed Opt 23:1-11
Wang, Guobao; Corwin, Michael T; Olson, Kristin A et al. (2018) Dynamic PET of human liver inflammation: impact of kinetic modeling with optimization-derived dual-blood input function. Phys Med Biol 63:155004
Winer, Rachel L; Tiro, Jasmin A; Miglioretti, Diana L et al. (2018) Rationale and design of the HOME trial: A pragmatic randomized controlled trial of home-based human papillomavirus (HPV) self-sampling for increasing cervical cancer screening uptake and effectiveness in a U.S. healthcare system. Contemp Clin Trials 64:77-87
Withers, Sita S; Skorupski, Katherine A; York, Daniel et al. (2018) Association of macrophage and lymphocyte infiltration with outcome in canine osteosarcoma. Vet Comp Oncol :
Floc'h, Nicolas; Martin, Matthew J; Riess, Jonathan W et al. (2018) Antitumor Activity of Osimertinib, an Irreversible Mutant-Selective EGFR Tyrosine Kinase Inhibitor, in NSCLC Harboring EGFR Exon 20 Insertions. Mol Cancer Ther 17:885-896
Weiss, Robert H (2018) Metabolomics and Metabolic Reprogramming in Kidney Cancer. Semin Nephrol 38:175-182
Shih, Tsung-Chieh; Liu, Ruiwu; Wu, Chun-Te et al. (2018) Targeting Galectin-1 Impairs Castration-Resistant Prostate Cancer Progression and Invasion. Clin Cancer Res 24:4319-4331
Arun, Adith S; Tepper, Clifford G; Lam, Kit S (2018) Identification of integrin drug targets for 17 solid tumor types. Oncotarget 9:30146-30162
Hegde, John V; Shaverdian, Narek; Daly, Megan E et al. (2018) Patient-reported quality-of-life outcomes after de-escalated chemoradiation for human papillomavirus-positive oropharyngeal carcinoma: Findings from a phase 2 trial. Cancer 124:521-529

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