FLOW CYTOMETRY & HIGH THROUGHPUT SCREENING SHARED RESOURCE ABSTRACT The UNMCC Flow Cytometry and High Throughput Screening Shared Resource (Flow Cytometry-HTS) provides UNMCC and other researchers with state-of-the-art instruments, infrastructure and expertise. The Resource, directed by Bruce Edwards, PhD and managed by Mark Carter, MS, provides cell analysis, cell sorting, and offline data analysis as standard services. Resource users are individually trained in instrument and software use. Current charge back rates to Resource users are in the low to mid-range of rates nationwide. The Resource also has a history of innovation: The Resource and members of the UNMCC Cancer Therapeutics (CT) Research Program (Sklar & B. Edwards) invented the capability to conduct high throughput screens using a flow cytometric platform (commercialized as HyperCyt in 2006), which led to creation of a full-fledged High Throughput Screening Center: The UNM Center for Molecular Discovery (CMD), which is affiliated with the Resource. In the past project period, the Resource has worked to facilitate access to CMD for UNMCC members as well as collaborators at other NCI Cancer Centers and HyperCyt high throughput screening as a technical service, allowing Resource users routine access to novel high throughput screening technologies. The Resource supports small scale screening projects for investigators who have already developed targets that are ready for screening in 96- or 384-well plates. The Resource also serves as a conduit for pilot projects funded from local resources to evolve into larger, NIH-supported screening projects in the CMD. This highly productive strategy has allowed UNMCC Program members to identify potent small molecule probes for estrogen receptors, GTPases, formyl peptide receptors and efflux transporters, as well as a number of approved drugs that could be repurposed for new clinical applications. The Resource also provides easily accessible reservation and billing systems. To promote excellence in research, the Resource provides UNMCC Program members and trainees with expert assistance and information to facilitate development of cell analysis and discovery projects in support of grant applications and publications. A Resource priority is to integrate advanced technologies developed by the CMD and UNMCC Research Programs into its operations to make them more broadly available to the research community. Education and dissemination activities include: participation in production of a UNM Shared Resource newsletter, workshops and presentations on specialized topics pertaining to flow cytometry and drug discovery, ad hoc facility tours for students and faculty, and a Resource web page to advertise upcoming events and Resource instruments, services and fees. During the previous 5-yr project period, 48 UNMCC members from 4 UNMCC Research Programs used the Resource, resulting in a total of 97 publications, of which 15 are presently pending PMCIDs. In the reporting year of July 2013 ? June 2014, UNMCC members were responsible for 86% of total Resource usage and were supported by 128 peer-reviewed grants.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
3P30CA118100-15S9
Application #
10230655
Study Section
Subcommittee I - Transistion to Independence (NCI)
Program Officer
Ptak, Krzysztof
Project Start
2005-09-26
Project End
2021-08-31
Budget Start
2019-09-01
Budget End
2020-08-31
Support Year
15
Fiscal Year
2020
Total Cost
Indirect Cost
Name
University of New Mexico Health Sciences Center
Department
Type
DUNS #
829868723
City
Albuquerque
State
NM
Country
United States
Zip Code
87131
Guo, Yan; Yu, Hui; Wang, Jing et al. (2018) The Landscape of Small Non-Coding RNAs in Triple-Negative Breast Cancer. Genes (Basel) 9:
Hatch, Ellen W; Geeze, Mary Beth; Martin, Cheyenne et al. (2018) Variability of PD-L1 expression in mastocytosis. Blood Adv 2:189-199
Frerich, Candace A; Brayer, Kathryn J; Painter, Brandon M et al. (2018) Transcriptomes define distinct subgroups of salivary gland adenoid cystic carcinoma with different driver mutations and outcomes. Oncotarget 9:7341-7358
Kinney, Anita Y; Howell, Rachel; Ruckman, Rachel et al. (2018) Promoting guideline-based cancer genetic risk assessment for hereditary breast and ovarian cancer in ethnically and geographically diverse cancer survivors: Rationale and design of a 3-arm randomized controlled trial. Contemp Clin Trials 73:123-135
Tasnim, Humayra; Fricke, G Matthew; Byrum, Janie R et al. (2018) Quantitative Measurement of Naïve T Cell Association With Dendritic Cells, FRCs, and Blood Vessels in Lymph Nodes. Front Immunol 9:1571
Leng, Shuguang; Diergaarde, Brenda; Picchi, Maria A et al. (2018) Gene Promoter Hypermethylation Detected in Sputum Predicts FEV1 Decline and All-Cause Mortality in Smokers. Am J Respir Crit Care Med 198:187-196
Castleman, Moriah J; Pokhrel, Srijana; Triplett, Kathleen D et al. (2018) Innate Sex Bias of Staphylococcus aureus Skin Infection Is Driven by ?-Hemolysin. J Immunol 200:657-668
Barton, Matthias; Filardo, Edward J; Lolait, Stephen J et al. (2018) Twenty years of the G protein-coupled estrogen receptor GPER: Historical and personal perspectives. J Steroid Biochem Mol Biol 176:4-15
Prossnitz, Eric R (2018) GPER modulators: Opportunity Nox on the heels of a class Akt. J Steroid Biochem Mol Biol 176:73-81
Perez, Dominique R; Nickl, Christian K; Waller, Anna et al. (2018) High-Throughput Flow Cytometry Identifies Small-Molecule Inhibitors for Drug Repurposing in T-ALL. SLAS Discov 23:732-741

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