The Markey Cancer Center (MCC) Flow Cytometry and Cell Sorting Shared Resource Facility (FCCS SRF) provides University of Kentucky (UK) cancer researchers with comprehensive, state-of-the-art analytical flow cytometry and cell sorting services to support both human and animal studies. The goals of the FCCS SRF are to provide: 1) multiparameter cell phenotyping;2) analysis of DNA content and cell cycle;3) analysis of cellular apoptosis and necrosis;4) quantification of cell cytotoxicity;5) intracellular cytokine analysis;6) measurement of cell activation parameters;7) high speed cell sorting of live human and animal cells under biocontainment conditions;and 8) single cell cloning of sorted cells. Another important function of the shared resource is to assist researchers in data analysis of flow cytometric studies and to help investigators in the development of new flow cytometric assays or approaches as needed for their research efforts. Finally, FCCS SRF addresses the future needs of investigators by adding new and updated instrumentation to the facility that will expand the number of techniques and approaches available to cancer researchers at the university.
Thirty-three cancer researchers from the four MCC research programs use FCCS SRF services for analysis and sorting of basic, clinical or translational research samples. FCCS SRF services offer cancer investigators the ability to assay phenotypic and functional characteristics of cells from normal and cancerous tissues. Value-added service from FCCS SRF has led to numerous publications and research grants from the NCI, other NIH institutes, and additional funding agencies.
|Jiang, Kai; Liu, Yajuan; Zhang, Jie et al. (2018) An intracellular activation of Smoothened that is independent of Hedgehog stimulation in Drosophila. J Cell Sci 131:|
|Dhar, Sanjit K; Bakthavatchalu, Vasudevan; Dhar, Bithika et al. (2018) DNA polymerase gamma (Pol?) deficiency triggers a selective mTORC2 prosurvival autophagy response via mitochondria-mediated ROS signaling. Oncogene 37:6225-6242|
|Engle, Jeff A; Traynor, Anne M; Campbell, Toby C et al. (2018) Assessment of adherence and relative dose intensity with oral chemotherapy in oncology clinical trials at an academic medical center. J Oncol Pharm Pract 24:348-353|
|Kim, Ji Tae; Napier, Dana L; Weiss, Heidi L et al. (2018) Neurotensin Receptor 3/Sortilin Contributes to Tumorigenesis of Neuroendocrine Tumors Through Augmentation of Cell Adhesion and Migration. Neoplasia 20:175-181|
|Gedaly, Roberto; De Stefano, Felice; Turcios, Lilia et al. (2018) mTOR Inhibitor Everolimus in Regulatory T cell Expansion for Clinical Application in Transplantation. Transplantation :|
|Pi, Fengmei; Binzel, Daniel W; Lee, Tae Jin et al. (2018) Nanoparticle orientation to control RNA loading and ligand display on extracellular vesicles for cancer regression. Nat Nanotechnol 13:82-89|
|Wang, Qingding; Zhou, Yuning; Rychahou, Piotr et al. (2018) Deptor Is a Novel Target of Wnt/?-Catenin/c-Myc and Contributes to Colorectal Cancer Cell Growth. Cancer Res 78:3163-3175|
|Rychahou, Piotr; Bae, Younsoo; Reichel, Derek et al. (2018) Colorectal cancer lung metastasis treatment with polymer-drug nanoparticles. J Control Release 275:85-91|
|Kosmac, Kate; Peck, Bailey D; Walton, R Grace et al. (2018) Immunohistochemical Identification of Human Skeletal Muscle Macrophages. Bio Protoc 8:|
|Frohman, Heather A; Rychahou, Piotr G; Li, Jing et al. (2018) Development of murine bariatric surgery models: lessons learned. J Surg Res 229:302-310|
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