Our original intent with the Mouse Core was to create a core that was rather different than other mouse cores. Our overarching goal was to use the Mouse Core to generate new transgenic and gene-targeted mouse lines that would allow us to test, as a group, fundamental problems in inner-ear biology. We intended therefore for the Mouse Core to have experfise in construct design and generafion, mouse husbandry, and genotyping. The reviewers of the first renewal submission easily picked up on the undeniable fact that we were far less successful than we had anficipated in making new mouse lines. Our original intent was to seed the construct-generation process by generating mouse lines that were more closely related to the interests of the Core PI, but that nevertheless tapped into topics of general interest to the OHRC group, such as mechanotransduction. However, the Mouse Core bogged down with construct generation and in obtaining germline transmission with its constructs. We are now at a position where we can begin to share the two generated mouse lines (TRPV5 and TRPV6 knock-ins) amongst the group, but we would have preferred to be here a few years ago. Moreover, the Mouse Core Scienfist, Jim Smart, who was capable of running all facets of the core, left for a more stable posifion, one with a long-term guarantee of employment. We had been lucky to get him at the salary we paid him, as he was quite experienced;we picked him straight out of graduate school, and were able to give him sufficient compensafion for the position to look attractive. That was not a long-term solufion for this position, however. This experience did point out that hiring someone with experience in making mouse lines was not easily done with the available P30 budget. In the meanfime, however, we had hired a Mouse Core Technician, Michael Bateschell, who demonstrated outstanding skill in the service-oriented aspects of the core. Combining the need to retain Bateschell and the plan to expand the core to included in utero electroporation, it became abundantly clear that we would not be able to hire someone for the core with sufficient molecular-genetics experience to be able to continue with the construct-generation service. The negative review of this core after its first renewal submission just reinforced this view.
| Wang, Lingyan; Kempton, J Beth; Brigande, John V (2018) Gene Therapy in Mouse Models of Deafness and Balance Dysfunction. Front Mol Neurosci 11:300 |
| Oh, Yonghee; Reiss, Lina A J (2018) Binaural Pitch Fusion: Effects of Amplitude Modulation. Trends Hear 22:2331216518788972 |
| Kachelmeier, Allan; Shola, Tsering; Meier, William B et al. (2018) Simplified, automated methods for assessing pixel intensities of fluorescently-tagged drugs in cells. PLoS One 13:e0206628 |
| Krey, Jocelyn F; Dumont, Rachel A; Wilmarth, Philip A et al. (2018) ELMOD1 Stimulates ARF6-GTP Hydrolysis to Stabilize Apical Structures in Developing Vestibular Hair Cells. J Neurosci 38:843-857 |
| Reiss, Lina A J; Fowler, Jennifer R; Hartling, Curtis L et al. (2018) Binaural Pitch Fusion in Bilateral Cochlear Implant Users. Ear Hear 39:390-397 |
| Reiss, Lina A J; Shayman, Corey S; Walker, Emily P et al. (2017) Binaural pitch fusion: Comparison of normal-hearing and hearing-impaired listeners. J Acoust Soc Am 141:1909 |
| Erickson, Timothy; Morgan, Clive P; Olt, Jennifer et al. (2017) Integration of Tmc1/2 into the mechanotransduction complex in zebrafish hair cells is regulated by Transmembrane O-methyltransferase (Tomt). Elife 6: |
| Krey, J F; Wilmarth, P A; David, L L et al. (2017) Analysis of the Proteome of Hair-Cell Stereocilia by Mass Spectrometry. Methods Enzymol 585:329-354 |
| Adler, Henry J; Anbuhl, Kelsey L; Atcherson, Samuel R et al. (2017) Community network for deaf scientists. Science 356:386-387 |
| Oh, Yonghee; Reiss, Lina A J (2017) Binaural pitch fusion: Pitch averaging and dominance in hearing-impaired listeners with broad fusion. J Acoust Soc Am 142:780 |
Showing the most recent 10 out of 165 publications
