The Genomics Core will be housed in the already-established Roy J. Carver Center for Genomics (CCG). This core will support technologically-advanced molecular biological research approaches, including genome-level analyses, needed for the study of inner ear development, physiology and disease. The goal of this core is to provide necessary expertise, technical resources and advanced instrumentation to allow users to identify and assess the underlying molecular processes and mechanisms of relevant inner ear biology. The scientific and technical expertise of the Director and Deputy Director is complementary and the half-time Research Assistant is an accomplished molecular biologist. Users will consult with the Director to determine appropriate methods and will work directly with the RA to develop and implement these procedures. Use of the extensive array of equipment in the CCG will be extended to all users of the Genomics Core. This includes: 2 ABI capillary DNA sequencers, an Experion electrophoresis system, microarray hybridization modules, a microarray scanner, 2 real-time PCR instruments (Roche &ABI), a flow-cytometer, and a Biomek robotic system with a microplate reader. The CCG can provide all necessary technical preparation work for """"""""next generation"""""""" high-throughput DNA sequencing using available instruments at U. Iowa &Iowa State U. (Roc he Genome Sequencer FLX System (aka """"""""454"""""""") and an lamina (aka """"""""Sol ex a"""""""") Genome Analyzer II).
The specific aims of the Genomics Core are: 1) To provide access to advanced molecular biology techniques and high-throughput instrumentation for auditory research. 2) To develop and facilitate research using quantitative analysis of spatio-temporal expresssion patterns of selected sets of auditory genes by quantitative RT-PCR and microarray methods. 3) To implement and support genome-wide approaches for auditory research to include high density microarrays and deep transcriptome sequencing. The Genomics Core will work closely with the Histology and Imaging Core to prepare, organize and store molecular probes for in situ analyses. In sum, the Genomics Core will provide key resources for conducting molecular aspects of hearing research at the University of Iowa.

Public Health Relevance

The Genomics Core provides centralized resources and facilities that are readily available to all NIDCD researchers at U. Iowa (currently 16 R01 funded investigators) as well as to other NIH-funded investigators at Iowa. The availability of this core will enhance ongoing NIDCD-funded research

National Institute of Health (NIH)
National Institute on Deafness and Other Communication Disorders (NIDCD)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZDC1)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Iowa
Iowa City
United States
Zip Code
Yang, Tian; Choi, Ji-Eun; Soh, Daniel et al. (2018) CaBP1 regulates Cav1 L-type Ca2+ channels and their coupling to neurite growth and gene transcription in mouse spiral ganglion neurons. Mol Cell Neurosci 88:342-352
Tuft, Bradley W; Xu, Linjing; Leigh, Braden et al. (2018) Photopolymerized micropatterns with high feature frequencies overcome chemorepulsive borders to direct neurite growth. J Tissue Eng Regen Med 12:e1392-e1403
Xu, Linjing; Seline, Alison E; Leigh, Braden et al. (2018) Photopolymerized Microfeatures Guide Adult Spiral Ganglion and Dorsal Root Ganglion Neurite Growth. Otol Neurotol 39:119-126
Leigh, Braden L; Truong, Kristy; Bartholomew, Reid et al. (2017) Tuning Surface and Topographical Features to Investigate Competitive Guidance of Spiral Ganglion Neurons. ACS Appl Mater Interfaces 9:31488-31496
Chagnaud, Boris P; Engelmann, Jacob; Fritzsch, Bernd et al. (2017) Sensing External and Self-Motion with Hair Cells: A Comparison of the Lateral Line and Vestibular Systems from a Developmental and Evolutionary Perspective. Brain Behav Evol 90:98-116
Yang, Tian; Scholl, Elizabeth S; Pan, Ning et al. (2016) Expression and Localization of CaBP Ca2+ Binding Proteins in the Mouse Cochlea. PLoS One 11:e0147495
Saito, Yuhki; Miranda-Rottmann, Soledad; Ruggiu, Matteo et al. (2016) NOVA2-mediated RNA regulation is required for axonal pathfinding during development. Elife 5:
Dvorakova, Martina; Jahan, Israt; Macova, Iva et al. (2016) Incomplete and delayed Sox2 deletion defines residual ear neurosensory development and maintenance. Sci Rep 6:38253
Fritzsch, Bernd; Duncan, Jeremy S; Kersigo, Jennifer et al. (2016) Neuroanatomical Tracing Techniques in the Ear: History, State of the Art, and Future Developments. Methods Mol Biol 1427:243-62
Li, Tongchao; Giagtzoglou, Nikolaos; Eberl, Daniel F et al. (2016) The E3 ligase Ubr3 regulates Usher syndrome and MYH9 disorder proteins in the auditory organs of Drosophila and mammals. Elife 5:

Showing the most recent 10 out of 95 publications