The Imaging Core provides access to knowledge and training allowing the user to learn and understand imaging technologies and sample preparation techniques that are tailored toward inner ear-related research. Beside standard equipment for sample preparation and microscopy, it also offers advanced imaging systems that are integrated into a physiology-oriented concept allowing access to a broad base of users of technology that is not available as off the shelf solution. The Imaging Core is integrated into the overall philosophy of the Stanford OHNS Core Center providing access to knowledge and technology (Aim 1), and to stimulate and inspire discussion among users to identify the best possible imaging solution for a specific question. Previous success supports the strategy that advanced scientific discussions foster and stimulate collaborative research (Aim 2). The core provides access and training for state-of-the-art confocal systems (Aim 3), each configured for specific tasks, such as routine confocal scanning in upright or inverted configurations, fast high resolution scanning, ultrafast scanning for imaging physiological processes in subcellular structures or spinning disk confocal imaging for living cells. In addition, users have access to a highly flexible 2-photon imaging system that allows imaging of isolated tissues and structures in living animals. For histology, the core offers access arid training to use apparatus for cryosectioning as well as paraffin embedding and sectioning.

Public Health Relevance

Access to equipment that requires expert maintenance and advanced training for optimal use of the individual instruments'capabilities can most efficiently be capitalized through the implemented Core Center. For an individual laboratory, it would be economically unbearable to maintain such broad and up-to-date capabilities. Implementing a philosophy where the user base is being educated to utilize the Core-provided technology in the best possible way creates a stimulating and innovative environment for collaborative research.

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Center Core Grants (P30)
Project #
2P30DC010363-06
Application #
8694348
Study Section
Special Emphasis Panel (ZDC1)
Project Start
Project End
Budget Start
2014-09-01
Budget End
2015-08-31
Support Year
6
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Stanford University
Department
Type
DUNS #
City
Stanford
State
CA
Country
United States
Zip Code
94304
Hartman, Byron H; B?scke, Robert; Ellwanger, Daniel C et al. (2018) Fbxo2VHC mouse and embryonic stem cell reporter lines delineate in vitro-generated inner ear sensory epithelia cells and enable otic lineage selection and Cre-recombination. Dev Biol 443:64-77
Dewey, James B; Xia, Anping; Müller, Ulrich et al. (2018) Mammalian Auditory Hair Cell Bundle Stiffness Affects Frequency Tuning by Increasing Coupling along the Length of the Cochlea. Cell Rep 23:2915-2927
Kim, Jinkyung; Xia, Anping; Grillet, Nicolas et al. (2018) Osmotic stabilization prevents cochlear synaptopathy after blast trauma. Proc Natl Acad Sci U S A 115:E4853-E4860
Edward, Justin A; Sanyal, Mrinmoy; Le, Wei et al. (2017) Selective expansion of human regulatory T cells in nasal polyps, and not adjacent tissue microenvironments, in individual patients exposed to steroids. Clin Immunol 179:66-76
Nguyen-Vu, Td Barbara; Zhao, Grace Q; Lahiri, Subhaneil et al. (2017) A saturation hypothesis to explain both enhanced and impaired learning with enhanced plasticity. Elife 6:
Jawadi, Zina; Applegate, Brian E; Oghalai, John S (2016) Optical Coherence Tomography to Measure Sound-Induced Motions Within the Mouse Organ of Corti In Vivo. Methods Mol Biol 1427:449-62
Xia, Anping; Liu, Xiaofang; Raphael, Patrick D et al. (2016) Hair cell force generation does not amplify or tune vibrations within the chicken basilar papilla. Nat Commun 7:13133
Lee, Hee Yoon; Raphael, Patrick D; Xia, Anping et al. (2016) Two-Dimensional Cochlear Micromechanics Measured In Vivo Demonstrate Radial Tuning within the Mouse Organ of Corti. J Neurosci 36:8160-73
Ealy, Megan; Ellwanger, Daniel C; Kosaric, Nina et al. (2016) Single-cell analysis delineates a trajectory toward the human early otic lineage. Proc Natl Acad Sci U S A 113:8508-13
Sundaresan, Srividya; Kong, Jee-Hyun; Fang, Qing et al. (2016) Thyroid hormone is required for pruning, functioning and long-term maintenance of afferent inner hair cell synapses. Eur J Neurosci 43:148-61

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