The Cytohistochemistry Core provides state-of-the-art technical support, facilities, and collaborative expertise for histochemical and morphological methods needed by DERC Affiliate Investigators. Cytohistochemistry Core services and facilities include tissue fixation, paraffin and cryostat tissue processing, histological and histochemical staining, immunocytochemistry, in situ hybridization, autoradiography, photomicrography and computer imaging, electron microscopy, and quantitative morphometric analysis. The Cytohistochemistry Core Director, Dr. Denis G. Baskin, is an established scientist in the fields of histochemistry and diabetes/endocrinology research. He is Research Professor of Medicine at the University of Washington and is also Senior Research Career Scientist in the VA Medical Research Service. The Core Director collaborates with DERC Affiliate Investigators and oversees the operations of the Core. In response to DERC Affiliate requests for electron microscopy Core support, the Cytohistochemistry Core recruited Dr. Thomas Wight to the position of Associate Core Director. Dr. Wight, a prominent cell biologist in the area of vascular biology and extracellular matrix, oversees the electron microscopy services of the Core, including a part-time technician. Charge backs are assessed to Core users to offset the cost of Core services. The Cytohistochemistry Core is a cost-efficient resource for a large group of DERC investigators and has been successful over the years in contributing to numerous publications by Affiliate Investigators. Going into the new funding period, the Cytohistochemistry Core has requests for service by 37 Affiliate Investigators for 40 projects, all with peer-reviewed grant support. The DERC also proposes to add a laser capture microdissection system to the Cytohistochemistry Core to provide Affiliates with the capability of obtaining microscopic samples of tissues and cells for molecular and biochemical analysis related to diabetes.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK017047-28
Application #
7550710
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
Budget Start
2003-12-01
Budget End
2004-11-30
Support Year
28
Fiscal Year
2004
Total Cost
$182,766
Indirect Cost
Name
University of Washington
Department
Type
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195
Mietlicki-Baase, Elizabeth G; Liberini, Claudia G; Workinger, Jayme L et al. (2018) A vitamin B12 conjugate of exendin-4 improves glucose tolerance without associated nausea or hypophagia in rodents. Diabetes Obes Metab 20:1223-1234
Gordon, Sharona E; Munari, Mika; Zagotta, William N (2018) Visualizing conformational dynamics of proteins in solution and at the cell membrane. Elife 7:
Olivo, Robert E; Davenport, Clemontina A; Diamantidis, Clarissa J et al. (2018) Obesity and synergistic risk factors for chronic kidney disease in African American adults: the Jackson Heart Study. Nephrol Dial Transplant 33:992-1001
Uusitalo, Ulla; Lee, Hye-Seung; Andrén Aronsson, Carin et al. (2018) Early Infant Diet and Islet Autoimmunity in the TEDDY Study. Diabetes Care 41:522-530
Brault, Michelle; Olsen, Tayla M; Martinez, Jennifer et al. (2018) Intracellular Nucleic Acid Sensing Triggers Necroptosis through Synergistic Type I IFN and TNF Signaling. J Immunol 200:2748-2756
Hofsteen, Peter; Robitaille, Aaron Mark; Strash, Nicholas et al. (2018) ALPK2 Promotes Cardiogenesis in Zebrafish and Human Pluripotent Stem Cells. iScience 2:88-100
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Subramanian, Savitha; Goodspeed, Leela; Wang, Shari et al. (2018) Deficiency of Invariant Natural Killer T Cells Does Not Protect Against Obesity but Exacerbates Atherosclerosis in Ldlr-/- Mice. Int J Mol Sci 19:
James, Eddie A; Pietropaolo, Massimo; Mamula, Mark J (2018) Immune Recognition of ?-Cells: Neoepitopes as Key Players in the Loss of Tolerance. Diabetes 67:1035-1042
Liese, Angela D; Ma, Xiaonan; Ma, Xiaoguang et al. (2018) Dietary quality and markers of inflammation: No association in youth with type 1 diabetes. J Diabetes Complications 32:179-184

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