ISLET CELL BIOLOGY CORE: Director - F. Matschinsky It is the purpose of this core to assist investigators who currently study or have plans to study independently or collaboratively various aspects of pancreatic islet cell biology. In order to accomplish this goal the Core provides four services. First, it will perform extra corporal phenotyping of the endocrine pancreas of mouse and rat using the intact isolated perfused or minced perifused pancreas. Second, it will isolate, culture, and functionally assess pancreatic islets of rat and mouse including batch incubations, perifusions, respirometry, measurements of Caj++, the P-potential (ATP, ADP, AMP and Pi) and other metabolites, hormone contents and release. Third, it maintains a broad and well characterized stock of transformed islet cells, grows large batches of such cells or generates pseudo islets by embedding such cells into agarose beads for dynamic studies of metabolism and hormone release. Fourth and most important, it provides in depth consultation and helps develop strategies how to use the services of the core optimally or will attempt to modify available technologies to solve particular problems. The islet cell resource permits the broad application of approaches which are too labor intensive and technically too demanding to be maintained by the average single laboratory. The core offers training to those who have the resources and wish to establish approved procedures and technologies in their own laboratory. Costs are reduced through a large scale operation. The Core functions thus as a research support and educational unit for an active group of investigators with somewhat diverse interests, and fosters interdisciplinary islet research.
Wooldridge, Amy L; Bischof, Robert J; Liu, Hong et al. (2018) Late-gestation maternal dietary methyl donor and cofactor supplementation in sheep partially reverses protection against allergic sensitization by IUGR. Am J Physiol Regul Integr Comp Physiol 314:R22-R33 |
Kim, Boa; Jang, Cholsoon; Dharaneeswaran, Harita et al. (2018) Endothelial pyruvate kinase M2 maintains vascular integrity. J Clin Invest 128:4543-4556 |
Gibson, Christopher E; Boodhansingh, Kara E; Li, Changhong et al. (2018) Congenital Hyperinsulinism in Infants with Turner Syndrome: Possible Association with Monosomy X and KDM6A Haploinsufficiency. Horm Res Paediatr 89:413-422 |
Hill, David A; Lim, Hee-Woong; Kim, Yong Hoon et al. (2018) Distinct macrophage populations direct inflammatory versus physiological changes in adipose tissue. Proc Natl Acad Sci U S A 115:E5096-E5105 |
Kim, Yong Hoon; Marhon, Sajid A; Zhang, Yuxiang et al. (2018) Rev-erb? dynamically modulates chromatin looping to control circadian gene transcription. Science 359:1274-1277 |
Mowel, Walter K; Kotzin, Jonathan J; McCright, Sam J et al. (2018) Control of Immune Cell Homeostasis and Function by lncRNAs. Trends Immunol 39:55-69 |
Rickels, Michael R; Peleckis, Amy J; Dalton-Bakes, Cornelia et al. (2018) Continuous Glucose Monitoring for Hypoglycemia Avoidance and Glucose Counterregulation in Long-Standing Type 1 Diabetes. J Clin Endocrinol Metab 103:105-114 |
Guan, Dongyin; Xiong, Ying; Borck, Patricia C et al. (2018) Diet-Induced Circadian Enhancer Remodeling Synchronizes Opposing Hepatic Lipid Metabolic Processes. Cell 174:831-842.e12 |
Jang, Cholsoon; Chen, Li; Rabinowitz, Joshua D (2018) Metabolomics and Isotope Tracing. Cell 173:822-837 |
Shoshkes-Carmel, Michal; Wang, Yue J; Wangensteen, Kirk J et al. (2018) Subepithelial telocytes are an important source of Wnts that supports intestinal crypts. Nature 557:242-246 |
Showing the most recent 10 out of 720 publications