- Molecular Genetics Core Given the value of model organisms and molecular genetic tools for the study of diabetes and its co- morbidities, the Molecular Genetics Core (MGC) is designed to aid diabetes researchers in the development of novel rodent models and molecular tools to determine the cellular and molecular mechanisms contributing to diabetes. Established in 2015, the MGC is a fee-for-service core that facilitates the application of molecular genetic methods to diabetes-related research. Specifically, the MGC (1) designs and produces genetically- modified rodent models (using CRISPR/Cas9) for use in diabetes-related research; (2) designs and produces AAV vectors for use in diabetes research; (3) produces and provides specialty viral reagents for use in diabetes research; and (4) provides advice and training in the use of these technologies to members of MDRC laboratories. The MG Core also owns and maintains several pieces of shared equipment for the use of MDRC members located at different sites around the UM medical campus. While CRISPR/Cas9 technology has dramatically increased the speed and decreased the cost at which such models can be generated, the pace at which this new technology continues to evolve prevents many diabetes researchers from taking full advantage of its potential. The MGC fills this gap by using its expertise and personnel to design and construct CRISPR/Cas9 targeting reagents, collaborate with the UM Transgenic Core to test these reagents in embryos and produce founder mice, and identify founders for transfer (along with genotyping protocols) to the MDRC investigator. For the generation of viral reagents, the MGC designs and produces any necessary constructs, which are packaged into viruses by the UM Viral Vector Core. With input from MDRC members and the MGC advisory committee, the MGC also identifies and develops new technologies (viral and genetic) in support of the research programs of MDRC members.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
2P30DK020572-41
Application #
9443256
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
Budget Start
2018-02-27
Budget End
2018-11-30
Support Year
41
Fiscal Year
2018
Total Cost
Indirect Cost
Name
University of Michigan Ann Arbor
Department
Type
DUNS #
073133571
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109
Zhao, Xu-Yun; Li, Siming; DelProposto, Jennifer L et al. (2018) The long noncoding RNA Blnc1 orchestrates homeostatic adipose tissue remodeling to preserve metabolic health. Mol Metab 14:60-70
Bodur, Cagri; Kazyken, Dubek; Huang, Kezhen et al. (2018) The IKK-related kinase TBK1 activates mTORC1 directly in response to growth factors and innate immune agonists. EMBO J 37:19-38
Calles-Escandón, Jorge; Koch, Kenneth L; Hasler, William L et al. (2018) Glucose sensor-augmented continuous subcutaneous insulin infusion in patients with diabetic gastroparesis: An open-label pilot prospective study. PLoS One 13:e0194759
Perng, Wei; Tang, Lu; Song, Peter X K et al. (2018) Metabolomic profiles and development of metabolic risk during the pubertal transition: a prospective study in the ELEMENT Project. Pediatr Res :
Hinder, Lucy M; Murdock, Benjamin J; Park, Meeyoung et al. (2018) Transcriptional networks of progressive diabetic peripheral neuropathy in the db/db mouse model of type 2 diabetes: An inflammatory story. Exp Neurol 305:33-43
Headen, Devon M; Woodward, Kyle B; Coronel, María M et al. (2018) Local immunomodulation with Fas ligand-engineered biomaterials achieves allogeneic islet graft acceptance. Nat Mater 17:732-739
Callaghan, Brian C; Gao, LeiLi; Li, Yufeng et al. (2018) Diabetes and obesity are the main metabolic drivers of peripheral neuropathy. Ann Clin Transl Neurol 5:397-405
Kumar, Navasuja; Pop-Busui, Rodica; Musch, David C et al. (2018) Central Corneal Thickness Increase Due to Stromal Thickening With Diabetic Peripheral Neuropathy Severity. Cornea 37:1138-1142
Mahany, Erica B; Han, Xingfa; Borges, Beatriz C et al. (2018) Obesity and High-Fat Diet Induce Distinct Changes in Placental Gene Expression and Pregnancy Outcome. Endocrinology 159:1718-1733
Citterio, Cintia E; Morishita, Yoshiaki; Dakka, Nada et al. (2018) Relationship between the dimerization of thyroglobulin and its ability to form triiodothyronine. J Biol Chem 293:4860-4869

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