Abstract

? Microscopy Imaging and Cellular Physiology Core The Microscopy Imaging and Cellular Physiology (MICP) Core provides members of the Michigan Diabetes Research Center access to state of the art microscopy imaging, in situ hybridization, and electrophysiologic (including optogenetics) analysis, along with expert analysis and support. For over 20 years, the MICPC has provided researchers use of confocal and wide-field microscopes to allow a wide array of cellular and tissue imaging techniques including; imaging of fixed tissues and cells, quantification, co-localization, and live cell imaging including FRET and FRAP experiments. We have now added in situ hybridization analysis with fluorescent or radioisotope or chromogenic substrates for localization and/or quantification of mRNA. The core also has also developed an electrophysiology laboratory that enables the analysis of electrical and ionic changes in neurons, islets, or other relevant tissues, including optogentic activation of these tissues. Core personnel provide extensive expertise in imaging, in situ hybridization and electrophysiology, enabling MDRC investigators to rapidly develop novel experimental ideas and obtain high quality results with expert analysis. Core personnel provide structured service, maintenance and expertise in imaging and cell physiology and related experiments to support diabetes research at Michigan.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK020572-43
Application #
9851844
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
Budget Start
2019-12-01
Budget End
2020-11-30
Support Year
43
Fiscal Year
2020
Total Cost
Indirect Cost

  Institution

Name
University of Michigan Ann Arbor
Department
Type
DUNS #
073133571
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109

  Publications

Prasad, Suchitra; Neef, Tobias; Xu, Dan et al. (2018) Tolerogenic Ag-PLG nanoparticles induce tregs to suppress activated diabetogenic CD4 and CD8 T cells. J Autoimmun 89:112-124
Mahajan, Anubha (see original citation for additional authors) (2018) Refining the accuracy of validated target identification through coding variant fine-mapping in type 2 diabetes. Nat Genet 50:559-571
Hussain, Syed Saad; Harris, Megan T; Kreutzberger, Alex J B et al. (2018) Control of insulin granule formation and function by the ABC transporters ABCG1 and ABCA1 and by oxysterol binding protein OSBP. Mol Biol Cell 29:1238-1257
Miller, Alison L; Gearhardt, Ashley N; Fredericks, Emily M et al. (2018) Targeting self-regulation to promote health behaviors in children. Behav Res Ther 101:71-81
Rumora, Amy E; Lentz, Stephen I; Hinder, Lucy M et al. (2018) Dyslipidemia impairs mitochondrial trafficking and function in sensory neurons. FASEB J 32:195-207
Woodworth, Hillary L; Perez-Bonilla, Patricia A; Beekly, Bethany G et al. (2018) Identification of Neurotensin Receptor Expressing Cells in the Ventral Tegmental Area across the Lifespan. eNeuro 5:
Orozco, Luz D; Farrell, Colin; Hale, Christopher et al. (2018) Epigenome-wide association in adipose tissue from the METSIM cohort. Hum Mol Genet 27:1830-1846
Muir, Lindsey A; Kiridena, Samadhi; Griffin, Cameron et al. (2018) Frontline Science: Rapid adipose tissue expansion triggers unique proliferation and lipid accumulation profiles in adipose tissue macrophages. J Leukoc Biol 103:615-628
Spencer, Michael S; Kieffer, Edith C; Sinco, Brandy et al. (2018) Outcomes at 18 Months From a Community Health Worker and Peer Leader Diabetes Self-Management Program for Latino Adults. Diabetes Care 41:1414-1422
Sujkowski, Alyson; Wessells, Robert (2018) Using Drosophila to Understand Biochemical and Behavioral Responses to Exercise. Exerc Sport Sci Rev 46:112-120

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