Abstract

? Microscopy Imaging and Cellular Physiology Core The Microscopy Imaging and Cellular Physiology (MICP) Core provides members of the Michigan Diabetes Research Center access to state of the art microscopy imaging, in situ hybridization, and electrophysiologic (including optogenetics) analysis, along with expert analysis and support. For over 20 years, the MICPC has provided researchers use of confocal and wide-field microscopes to allow a wide array of cellular and tissue imaging techniques including; imaging of fixed tissues and cells, quantification, co-localization, and live cell imaging including FRET and FRAP experiments. We have now added in situ hybridization analysis with fluorescent or radioisotope or chromogenic substrates for localization and/or quantification of mRNA. The core also has also developed an electrophysiology laboratory that enables the analysis of electrical and ionic changes in neurons, islets, or other relevant tissues, including optogentic activation of these tissues. Core personnel provide extensive expertise in imaging, in situ hybridization and electrophysiology, enabling MDRC investigators to rapidly develop novel experimental ideas and obtain high quality results with expert analysis. Core personnel provide structured service, maintenance and expertise in imaging and cell physiology and related experiments to support diabetes research at Michigan.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK020572-44
Application #
10071179
Study Section
Special Emphasis Panel (ZDK1)
Project Start
1996-12-01
Project End
2022-11-30
Budget Start
2020-12-01
Budget End
2021-11-30
Support Year
44
Fiscal Year
2021
Total Cost
Indirect Cost

  Institution

Name
University of Michigan Ann Arbor
Department
Type
DUNS #
073133571
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109

  Publications

Hinder, Lucy M; Murdock, Benjamin J; Park, Meeyoung et al. (2018) Transcriptional networks of progressive diabetic peripheral neuropathy in the db/db mouse model of type 2 diabetes: An inflammatory story. Exp Neurol 305:33-43
Zhao, Xu-Yun; Li, Siming; DelProposto, Jennifer L et al. (2018) The long noncoding RNA Blnc1 orchestrates homeostatic adipose tissue remodeling to preserve metabolic health. Mol Metab 14:60-70
Bodur, Cagri; Kazyken, Dubek; Huang, Kezhen et al. (2018) The IKK-related kinase TBK1 activates mTORC1 directly in response to growth factors and innate immune agonists. EMBO J 37:19-38
Calles-Escandón, Jorge; Koch, Kenneth L; Hasler, William L et al. (2018) Glucose sensor-augmented continuous subcutaneous insulin infusion in patients with diabetic gastroparesis: An open-label pilot prospective study. PLoS One 13:e0194759
Perng, Wei; Tang, Lu; Song, Peter X K et al. (2018) Metabolomic profiles and development of metabolic risk during the pubertal transition: a prospective study in the ELEMENT Project. Pediatr Res :
Citterio, Cintia E; Morishita, Yoshiaki; Dakka, Nada et al. (2018) Relationship between the dimerization of thyroglobulin and its ability to form triiodothyronine. J Biol Chem 293:4860-4869
Headen, Devon M; Woodward, Kyle B; Coronel, María M et al. (2018) Local immunomodulation with Fas ligand-engineered biomaterials achieves allogeneic islet graft acceptance. Nat Mater 17:732-739
Callaghan, Brian C; Gao, LeiLi; Li, Yufeng et al. (2018) Diabetes and obesity are the main metabolic drivers of peripheral neuropathy. Ann Clin Transl Neurol 5:397-405
Kumar, Navasuja; Pop-Busui, Rodica; Musch, David C et al. (2018) Central Corneal Thickness Increase Due to Stromal Thickening With Diabetic Peripheral Neuropathy Severity. Cornea 37:1138-1142
Mahany, Erica B; Han, Xingfa; Borges, Beatriz C et al. (2018) Obesity and High-Fat Diet Induce Distinct Changes in Placental Gene Expression and Pregnancy Outcome. Endocrinology 159:1718-1733

Showing the most recent 10 out of 1823 publications