The Mass Spectrometry (MS) Core of the Washington University Diabetes Research Center (DRC) increases DRC efficiency and cost effectiveness by performing MS analyses for affiliated investigators. The MS Core provides state-of-the-art MS services to determine structures and to quantitate amounts of diabetes-related biomolecules. The MS Core provides centralized, standardized analytical procedures that permit study of molecular mechanisms of the pathogenesis of diabetes mellitus, its risk factors, and its complications, including the metabolic syndrome, obesity, atherosclerosis, and increased susceptibility to infections. Speciflc objectives of the DRC MS Core are: 1. To provide training to students and fellows in principles of MS and use of MS systems, e.g., gas chromatography (GC)/MS, isotope ratio (IR)/MS, electrospray ionization (ESI)/tandem MS, and matrix assisted laser desorption ionization (MALDI)/time of fiight/(TOF)/MS in analyses of diabetes-related biomolecules. To develop new MS methods for structural identification and quantitation of molecules involved in diabetes, its risk factors and complications, and related physiologic and pathophysiologic events. To perform service MS analyses, e.g. quantitation of target analytes and obtaining spectra for structural i identification, for DRTC Investigators. To assist DRC-affiliated investigators in developing MS assays. To provide consultation in interpreting MS data. To develop and disseminate new approaches in biomedical MS that are applicable to diabetes research. To provide and maintain functional MS systems for use in diabetes research. To perform collaborative diabetes research studies involving MS Core staff requiring specialized expertise, e.g.. Stable Isotope Labeling in Cell Culture (SILAC), Stable Isotpe Labeling Tandem Mass Spectrometry (SILT), characterization of post-translational modifications or protein-protein interactions, or studies of complex lipids that require de novo structure determination. 9. To reduce diabetes research costs by providing centralized MS services at a fraction of the cost of commercial MS services or of maintaining instruments in the laboratories of individual investigators.

Public Health Relevance

The DRC MS Core employs the power of mass spectrometry to study biochemical and metabolic pathways that are altered in diabetes and in its risk factors and complications, including obesity, cardiovascular disease, and increased suscpetibility to infections, which cause morbidity and accelerate mortality. Insight into their mechanisms may lead to more effective means to prevent or treat them.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK020579-37
Application #
8625739
Study Section
Special Emphasis Panel (ZDK1-GRB-S)
Project Start
Project End
Budget Start
2013-12-01
Budget End
2014-11-30
Support Year
37
Fiscal Year
2014
Total Cost
$191,471
Indirect Cost
$65,503
Name
Washington University
Department
Type
DUNS #
068552207
City
Saint Louis
State
MO
Country
United States
Zip Code
63130
Musselman, Laura Palanker; Fink, Jill L; Maier, Ezekiel J et al. (2018) Seven-Up Is a Novel Regulator of Insulin Signaling. Genetics 208:1643-1656
Riek, Amy E; Oh, Jisu; Darwech, Isra et al. (2018) Vitamin D3 supplementation decreases a unique circulating monocyte cholesterol pool in patients with type 2 diabetes. J Steroid Biochem Mol Biol 177:187-192
Peterson, Linda R; Xanthakis, Vanessa; Duncan, Meredith S et al. (2018) Ceramide Remodeling and Risk of Cardiovascular Events and Mortality. J Am Heart Assoc 7:
Bittel, Adam J; Bohnert, Kathryn L; Reeds, Dominic N et al. (2018) Reduced Muscle Strength in Barth Syndrome May Be Improved by Resistance Exercise Training: A Pilot Study. JIMD Rep :
Hampton, Kaia K; Anderson, Katie; Frazier, Hilaree et al. (2018) Insulin Receptor Plasma Membrane Levels Increased by the Progesterone Receptor Membrane Component 1. Mol Pharmacol 94:665-673
Ferguson, Daniel; Blenden, Mitchell; Hutson, Irina et al. (2018) Mouse Embryonic Fibroblasts Protect ob/ob Mice From Obesity and Metabolic Complications. Endocrinology 159:3275-3286
Samovski, Dmitri; Dhule, Pallavi; Pietka, Terri et al. (2018) Regulation of Insulin Receptor Pathway and Glucose Metabolism by CD36 Signaling. Diabetes 67:1272-1284
Warren, Junco S; Tracy, Christopher M; Miller, Mickey R et al. (2018) Histone methyltransferase Smyd1 regulates mitochondrial energetics in the heart. Proc Natl Acad Sci U S A 115:E7871-E7880
Funk, Steven D; Bayer, Raymond H; Malone, Andrew F et al. (2018) Pathogenicity of a Human Laminin ?2 Mutation Revealed in Models of Alport Syndrome. J Am Soc Nephrol 29:949-960
Adams, Melissa T; Gilbert, Jennifer M; Hinojosa Paiz, Jesus et al. (2018) Endocrine cell type sorting and mature architecture in the islets of Langerhans require expression of Roundabout receptors in ? cells. Sci Rep 8:10876

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