The Mass Spectrometry (MS) Core of the Washington University Diabetes Research Center (DRC) increases DRC efficiency and cost effectiveness by performing MS analyses for affiliated investigators. The MS Core provides state-of-the-art MS services to determine structures and to quantitate amounts of diabetes-related biomolecules. The MS Core provides centralized, standardized analytical procedures that permit study of molecular mechanisms of the pathogenesis of diabetes mellitus, its risk factors, and its complications, including the metabolic syndrome, obesity, atherosclerosis, and increased susceptibility to infections. Speciflc objectives of the DRC MS Core are: 1. To provide training to students and fellows in principles of MS and use of MS systems, e.g., gas chromatography (GC)/MS, isotope ratio (IR)/MS, electrospray ionization (ESI)/tandem MS, and matrix assisted laser desorption ionization (MALDI)/time of fiight/(TOF)/MS in analyses of diabetes-related biomolecules. To develop new MS methods for structural identification and quantitation of molecules involved in diabetes, its risk factors and complications, and related physiologic and pathophysiologic events. To perform service MS analyses, e.g. quantitation of target analytes and obtaining spectra for structural i identification, for DRTC Investigators. To assist DRC-affiliated investigators in developing MS assays. To provide consultation in interpreting MS data. To develop and disseminate new approaches in biomedical MS that are applicable to diabetes research. To provide and maintain functional MS systems for use in diabetes research. To perform collaborative diabetes research studies involving MS Core staff requiring specialized expertise, e.g.. Stable Isotope Labeling in Cell Culture (SILAC), Stable Isotpe Labeling Tandem Mass Spectrometry (SILT), characterization of post-translational modifications or protein-protein interactions, or studies of complex lipids that require de novo structure determination. 9. To reduce diabetes research costs by providing centralized MS services at a fraction of the cost of commercial MS services or of maintaining instruments in the laboratories of individual investigators.
The DRC MS Core employs the power of mass spectrometry to study biochemical and metabolic pathways that are altered in diabetes and in its risk factors and complications, including obesity, cardiovascular disease, and increased suscpetibility to infections, which cause morbidity and accelerate mortality. Insight into their mechanisms may lead to more effective means to prevent or treat them.
|Musselman, Laura Palanker; Fink, Jill L; Maier, Ezekiel J et al. (2018) Seven-Up Is a Novel Regulator of Insulin Signaling. Genetics 208:1643-1656|
|Riek, Amy E; Oh, Jisu; Darwech, Isra et al. (2018) Vitamin D3 supplementation decreases a unique circulating monocyte cholesterol pool in patients with type 2 diabetes. J Steroid Biochem Mol Biol 177:187-192|
|Peterson, Linda R; Xanthakis, Vanessa; Duncan, Meredith S et al. (2018) Ceramide Remodeling and Risk of Cardiovascular Events and Mortality. J Am Heart Assoc 7:|
|Bittel, Adam J; Bohnert, Kathryn L; Reeds, Dominic N et al. (2018) Reduced Muscle Strength in Barth Syndrome May Be Improved by Resistance Exercise Training: A Pilot Study. JIMD Rep :|
|Hampton, Kaia K; Anderson, Katie; Frazier, Hilaree et al. (2018) Insulin Receptor Plasma Membrane Levels Increased by the Progesterone Receptor Membrane Component 1. Mol Pharmacol 94:665-673|
|Ferguson, Daniel; Blenden, Mitchell; Hutson, Irina et al. (2018) Mouse Embryonic Fibroblasts Protect ob/ob Mice From Obesity and Metabolic Complications. Endocrinology 159:3275-3286|
|Samovski, Dmitri; Dhule, Pallavi; Pietka, Terri et al. (2018) Regulation of Insulin Receptor Pathway and Glucose Metabolism by CD36 Signaling. Diabetes 67:1272-1284|
|Warren, Junco S; Tracy, Christopher M; Miller, Mickey R et al. (2018) Histone methyltransferase Smyd1 regulates mitochondrial energetics in the heart. Proc Natl Acad Sci U S A 115:E7871-E7880|
|Funk, Steven D; Bayer, Raymond H; Malone, Andrew F et al. (2018) Pathogenicity of a Human Laminin ?2 Mutation Revealed in Models of Alport Syndrome. J Am Soc Nephrol 29:949-960|
|Adams, Melissa T; Gilbert, Jennifer M; Hinojosa Paiz, Jesus et al. (2018) Endocrine cell type sorting and mature architecture in the islets of Langerhans require expression of Roundabout receptors in ? cells. Sci Rep 8:10876|
Showing the most recent 10 out of 654 publications