Abstract

The Epithelial Cell and Mucosal Immunology Core (Core C) provides the infrastructure, biologic resources, expertise, and training for innovative and cross-disciplinary research on epithelial biology and immune function in mucosal surfaces and solid organs of the GI tract. Specific activities and services include: Polarized Epithelial Cell Culture and Genetic Transformations nCounter NanoString RNA Analysis FACs 7- or 12-Color Analysis Of Cultured Cells or Tissue/Organoid-derived Primary Cells Transepithelial Electrophysiology of live cells, cell monolayers, and tissues Gastrointestinal Organoid Derivation and Culture Specialized Equipment, Reagents, Training, and Consultation for recombinant protein expression and purification, including small molecules, specialized and unique epithelial cell lines, expression profiling using Bio-Plex MAGPIX multiplex reader Research and Development of live cell ratio fluorescence imaging of ion and solute transients, and FRAP analysis of membrane molecules A total of 27 HDDC members and 20 non-Members used Core C in the last grant cycle as measured in billable hours of service. 8 HDDC Members used Core C services in every quarter. The Core recorded >4000 billable hours/year. 45 Members and Associate Members anticipate use of Core C in the next grant cycle. All services will be utilized.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK034854-34
Application #
9605283
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
Budget Start
2018-12-01
Budget End
2019-11-30
Support Year
34
Fiscal Year
2019
Total Cost
Indirect Cost

  Institution

Name
Boston Children's Hospital
Department
Type
DUNS #
076593722
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

Gornati, Laura; Zanoni, Ivan; Granucci, Francesca (2018) Dendritic Cells in the Cross Hair for the Generation of Tailored Vaccines. Front Immunol 9:1484
Blumberg, Richard S; Lillicrap, David; IgG Fc Immune Tolerance Group (2018) Tolerogenic properties of the Fc portion of IgG and its relevance to the treatment and management of hemophilia Blood 131:2205-2214
Yao, Lina; Seaton, Sarah Craven; Ndousse-Fetter, Sula et al. (2018) A selective gut bacterial bile salt hydrolase alters host metabolism. Elife 7:
Haghighi, Alireza; Krier, Joel B; Toth-Petroczy, Agnes et al. (2018) An integrated clinical program and crowdsourcing strategy for genomic sequencing and Mendelian disease gene discovery. NPJ Genom Med 3:21
Lee, Christine K; Mitchell, Paul D; Raza, Roshan et al. (2018) Validation of Transient Elastography Cut Points to Assess Advanced Liver Fibrosis in Children and Young Adults: The Boston Children's Hospital Experience. J Pediatr 198:84-89.e2
Santus, William; Mingozzi, Francesca; Vai, Marina et al. (2018) Deep Dermal Injection As a Model of Candida albicans Skin Infection for Histological Analyses. J Vis Exp :
Stein, Richard R; Tanoue, Takeshi; Szabady, Rose L et al. (2018) Computer-guided design of optimal microbial consortia for immune system modulation. Elife 7:
Chen, Peng; Tao, Liang; Wang, Tianyu et al. (2018) Structural basis for recognition of frizzled proteins by Clostridium difficile toxin B. Science 360:664-669
Shaw, Kelly A; Cutler, David J; Okou, David et al. (2018) Genetic variants and pathways implicated in a pediatric inflammatory bowel disease cohort. Genes Immun :
Lyons, Jesse; Ghazi, Phaedra C; Starchenko, Alina et al. (2018) The colonic epithelium plays an active role in promoting colitis by shaping the tissue cytokine profile. PLoS Biol 16:e2002417

Showing the most recent 10 out of 869 publications